[Histonet] Control Slides

[Sebree Linda A.]

Speaking only as a tech that performs IHC and ISH, we use our own
control slides whenever possible unless we have been unable to find
positive control tissue, i.e. adenovirus.  We not only review the
positive control tissue but the patient as well just in case there is an
unexpected result on the patient tissue that needs investigating.  Even
in cases where the control tissue is on the same slide as the patient
tissue, there have been instances where one has stained appropriately
and the other has not.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kevin
and Chantel Bosewell
Sent: Wednesday, August 31, 2005 3:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Control Slides


Hello All,
    Our Pathologist believe control slides which are purchased are bomb
proof and we should process our own.  How does everyone else feel about
this? Oh, and one more question,  when you do any special stain do you
review the patient slide or just the control?  I have been asking around
and I have been getting different answers.  We recently had a false
negative on a GMS for Fungus and this tech does not review the patient
slides, in this case the control slide was positive.  Thank you for any
insight.

Chantel Boswell HT (ASCP)
Waco, Tx
Thank you in advance,
Chantel Boswell HT (ASCP)
Waco, Tx
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