[Histonet] can PBS cause background?

[Carolyn Johnson]

Hello all,

I have been struggling with high background in frozen mouse sections
processed for immunofluorescence.  A colleague told me that switching from
PBS to a Tris buffer reduced their non-specific background.  Does anyone
know why this would make a difference?  Also, I have tried using 1% sodium
borohydride for my paraformaldehyde fixed sections, but the slide mounted
tissue trapped gas bubbles and made it wrinkly.  Is there a way to avoid the
bubbles under the tissue?

Thanks!

 

Carolyn Johnson

Research Assistant

The Rockefeller University

1230 York Ave, Box 275

New York, NY 10021

Phone:(212) 327-8672

Fax:(212) 327-8664

 <mailto:cjohnson <@t> rockefeller.edu> cjohnson <@t> rockefeller.edu