[Histonet] can PBS cause background?
Carolyn Johnson
cjohnson <@t> mail.rockefeller.edu
Tue Aug 30 12:03:38 CDT 2005
Hello all,
I have been struggling with high background in frozen mouse sections
processed for immunofluorescence. A colleague told me that switching from
PBS to a Tris buffer reduced their non-specific background. Does anyone
know why this would make a difference? Also, I have tried using 1% sodium
borohydride for my paraformaldehyde fixed sections, but the slide mounted
tissue trapped gas bubbles and made it wrinkly. Is there a way to avoid the
bubbles under the tissue?
Thanks!
Carolyn Johnson
Research Assistant
The Rockefeller University
1230 York Ave, Box 275
New York, NY 10021
Phone:(212) 327-8672
Fax:(212) 327-8664
<mailto:cjohnson <@t> rockefeller.edu> cjohnson <@t> rockefeller.edu
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