[Histonet] Omega Chemical's DECAL

[John A. Kiernan]

Should the claim, "... a 24 hour 'turnaround' to 
fully decalcify a Mouse Head completely & doesn't 
interfere in Immuno" be believed? Immuno for
every antigen? If it decalcifies things rapidly 
it is almost certainly a quite concentrated acid,
and may cause chemical changes such as Feulgen
hydrolysis of DNA and extraction of RNA. Is the
product's composition disclosed, or is it a
secret brew? It's extremely easy to make 
decalcifying solutions in the lab from simple,
inexpensive ingredients, following well tried
and understood published recipes.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Bruce Abaloz wrote:
> 
> Dear Histonetters,
> 
> Am wondering whether any of you, know of the product Omega Chemical's
> DECAL, which a PhD student in  my lab. has informed me of. HAS a 24
> hour 'turnaround' to fully decalcify a Mouse Head completely &
> doesn't interfere in Immuno.
> We have been using RDO successfully but even the potent RDO took 3
> days to fully decalcify a mouse head & a 'Faster' turnaround' would
> be appreciated. Thankyou in advance for your advice,
> 
> Bruce in OZ (on behalf of Nanette Schneider from Deutschland)
> 
> --
>   BRUCE ABALOZ                                PH:61383446282
>   HISTOLOGIST                                 FAX:61383447909
>   DEPT.of ZOOLOGY                             EMAIL: brucea <@t> unimelb.edu.au
>   THE UNIVERSITY Of MELBOURNE.
>   VICTORIA.AUSTRALIA 3010
> http://www.zoology.unimelb.edu.au/