[Histonet] Cutting defect and blade adjustement

Alan Bright abright <@t> brightinstruments.com
Tue Aug 30 06:15:48 CDT 2005


Jorge,

You do not say if these are vertical lines or horizontal. If they are
vertical then you have a small nick in the knife, simply slide the knife
to a new position. If the lines are horizontal then it could be a number
of things. Please let me know.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright <@t> brightinstruments.com
Web Site: www.brightinstruments.com



-----Original Message-----
From: Jorge Tornero [mailto:jorge.tornero <@t> gmail.com] 
Sent: 30 August 2005 11:04
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cutting defect and blade adjustement


Please help me about the right knife angle for cutting technovit 7100
blocks with a leica tungsten carbide knife. I use a leica RM 2145
microtome.

I am using a freshly sharpened knife, but I get some sharp lines on the
cuttings (you know, those that appear all the way long in the cut and in
the same place) anyway. It is the first time that the knife is
sharpened. Also I hear a buzzing noise when I cut, it seems like if the
block causes some vibration in the blade, but it is well fastened. I
have tried to decrease speed when cutting but it doesn't work at all.
Now I am using a kind of moulds bigger than those that I used before, I
don't know if I should trim the block edge to make it narrower and ease
the cutting this way. any suggestions, please?

Thank you very much

Jorge Tornero
IEO-CADIZ
Spain

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