[Histonet] Cutting defect and blade adjustement

[Jorge Tornero]

My blocks are 12 mm wide by 16 mm long, and they are about 5 mm thick.
They are glued (with technovit 3090) to a small piece of wood and this
is the piece that I hold with the specimen holder. I am sectioning
anchovy ovaries, and I am using a non standard protocol for the
embedding, with much longer processing times, I guess because of the
very high water content of the tissue. I wonder if it is better to
make more changes in the alcohols instead of long times.

I am sorry but no way for using glass knives or diamond (budgets)

Thank you very much for your responses, I am waiting for more if you
want, any help would be appreciated

Jorge Tornero
IEO-CADIZ
Spain

2005/8/30, meyenhmf <@t> umdnj.edu <meyenhmf <@t> umdnj.edu>:
> How big are your blocks? Thickness?Tissue?
> I would use glass knives or Diamond.
> Metal blades always give streaks.
> Regards,
> Markus
> 
> 
> 
> forQuoting Jorge Tornero <jorge.tornero <@t> gmail.com>:
> 
> > Please help me about the right knife angle for cutting technovit 7100
> > blocks with a leica tungsten carbide knife. I use a leica RM 2145
> > microtome.
> >
> > I am using a freshly sharpened knife, but I get some sharp lines on
> > the cuttings (you know, those that appear all the way long in the cut
> > and in the same place) anyway. It is the first time that the knife is
> > sharpened. Also I hear a buzzing noise when I cut, it seems like if
> > the block causes some vibration in the blade, but it is well fastened.
> > I have tried to decrease speed when cutting but it doesn't work at
> > all. Now I am using a kind of moulds bigger than those that I used
> > before, I don't know if I should trim the block edge to make it
> > narrower and ease the cutting this way. any suggestions, please?
> >
> > Thank you very much
> >
> > Jorge Tornero
> > IEO-CADIZ
> > Spain
> >
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> 
> 
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> 
>