[Histonet] Michel's medium for skin biopsies

[mucram11 <@t> comcast.net]

Hi Paul,

Michel's is a transport media not a fixative as Robyn indicated.  It is similar to Zeus in some components.  It really depends on what you need a fixative or something to move the tissue and preserve the antigenic sites during that process.  The transport media are generally only good for holding 24 to 48 hours with most recommending 24 hours or less for EM.

Good Luck, 
Pam Marcum

-------------- Original message -------------- 

> Paul, 
> I believe Michels is a transport media, not a fixative media. You don't want 
> the tissue fixed, if you are doing antibody testing on it, in the fresh tissue 
> sense. I use Zeus Tissue Transport Media, my doctor seems to like it better. 
> 
> Robyn 
> OHSU 
> 
> >>> "Atkinson Paul B" 8/24/2005 2:18 AM >>> 
> 
> We are considering using Michels's transport medium for skin biopsies in our 
> laboratory. Have other laboratories found its use worthwhile? The original 
> 1973 paper only mentions the effect on immunoglobulin staining, is it also 
> effective in preserving complement antigenicity? Also how long does the 
> tissue need to be in the fluid for the 'fixation' to occur? 
> 
> Many thanks 
> 
> Paul Atkinson 
> 
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