[Histonet] Breast Processing Protocol
DDDeltour <@t> mar.med.navy.mil
DDDeltour <@t> mar.med.navy.mil
Wed Aug 17 08:58:22 CDT 2005
I am looking for a program for processing fatty breast tissue. We currently
let the breast sit in cassettes in Pen-Fix for an extra day before
processing. We then process the first Fixative in Pen-Fix for one hour then
in 10% Formalin for the second hour (I do not like this but that is they way
it was done when I got here). The next steps are.. 95% one hour X3, 100% one
hour X3, Xylene 1 hour X2. Paraffin for one hour X3. I am considering
replacing one of the 95% with another fixative. What programs do you use for
this? They don't like to hear Sh in Sh out:-). Thank you!
DOUGLAS D. DELTOUR
HISTOLOGY TECHNICIAN
NMC PORTSMOUTH VA
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