[Histonet] Apoptosis detection in plastic embedded tissue

[Eijl, drs. T.J.A. van]

Dear histonetters,
 
We have been trying to stain apoptotic cells in Carnoy fixed, BMA/MMA embedded mouse lungs using the chromogenic DEAD-END Kit by Promega. However, this kit has been designed for formalin fixed, parafin embedded tissue. To cut a long story short, we did not get it to work properly.
Now I am enquiring if anybody has knowledge of an apoptosis detection method that can be used on plastic embedded tissues as described above.
 
Thanks in advance,
 
Sven van Eijl
Ph.D. student
Dept. of Pharmacology & Pathophysiology
UIPS
The Netherlands