[Histonet] Reference on shrinkage of bone histological preparations

[Gayle Callis]

For those interested in publication on bone shrinkage during histological 
preparation,

Lane J, Ralis ZA.  Changes in dimensions of large cancellous bone specimens 
during histological preparation as measured on slabs from human femoral 
heads.  Calcif Tissue Int (1983) 35:1-4.

Although an old publication, it was interesting to note how they did the 
study, and measurements taken after

fixation
decalcification
water rinses
processing
	a. dehydration in alcohols
	b. clearing agent
embedding in paraplast
after cutting 8 and 14 um sections
after staining
Total shrinkage after all these factors







Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)