[Histonet] fixation of fresh frozen section

[Edmondson David (RBV) NHS Christie Tr]

Hi
I recently  heard a presentation where they spoke of Periodate-Lysine-Paraformaldehyde in the context of Frozen sections.   The coagulant fixatives do not alter the proteins chemistry so on might suggest no loss of antigenicity, the "Gold Standard".   But the morphology of PLP is much better.   It is fifteen and more years since I used it myself and maybe a search would be a  quicker way to find the concentrations.   I ahve an email contact if you need to ask him.
David
Christie
Manchester UK
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Bruijntjes, J.P.
Sent: 03 August 2005 14:39
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] fixation of fresh frozen section


Hi all

 

When I do immunocytochemistry on fresh frozen section I fix most, if not
all slides with acetone. Sometimes I use cold methanol because the
datasheet tells me to do so. Is anyone of you who prefer other
fixatives?

 

Joost Bruijntjes

TNO Quality of Life

Zeist

Holland

 


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