[Histonet] Re: fixation of fresh frozen section

Wed Aug 3 11:22:34 CDT 2005

Joost,

What are you staining for, i.e. what antigens?  That determines what 
fixative we choose to use.

At 07:39 AM 8/3/2005, you wrote:
>Hi all
>
>
>
>When I do immunocytochemistry on fresh frozen section I fix most, if not
>all slides with acetone. Sometimes I use cold methanol because the
>datasheet tells me to do so. Is anyone of you who prefer other
>fixatives?
>Joost Bruijntjes
>TNO Quality of Life
>Zeist
>Holland
>
>

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)