[Histonet] Re: ooops, rat on rat

[Gayle Callis]

You could use a biotinylated primary, as in the DAKO ARK methodology, 
eliminate the secondary to avoid having some "host" antiRat detect not only 
the rat primary, but also bind to rat tissue, then come back with a 
Strepavidin-HRP or AP.  Sometimes you have to dilute out the primary more -

I am not sure if anyone has a rat on rat kit, try Scytek out of Logan UT, 
you might get lucky on a kit.

At 01:49 PM 8/2/2005, you wrote:
>Okay, I knew I'd say that wrong. It is a rat anti-mouse antibody to be
>used on rat tissues. How would you handle that?
>
>
>
>Renee' Till, HT
>
>
>
>Arkansas Childrens Nutrition Center
>
>1120 Marshall
>
>Little Rock, AR
>
>72202
>
>
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>(501) 364-2774
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)