[Histonet] RE: Histonet Digest, Vol 17, Issue 38 - Heinz body stain

[Malam Jacqueline]

I have a Heinz body stain for you:
Rhodinile blue (Simpson, Carlisle, Mallard 1970)
Working solution:
0.5g rhodinile blue (E. Gurr, Michrome No 1156) to 100ml of a 1% solution of
aqueous NaCl. Shake well and filter through No 1 Whatman filter paper.
Method:
To a few drops of heparinised or EDTA-treated blood in a small vial, add the
same amount of working solution of stain.
Swirl gently and stand for 2 mins (the stain - not you!!).
Prepare thin smears and air dry.
Examine as usual or mount in resin mountant.
Results:
Heinz bodies - deep purple
Erythrocytes - yellow-orange to blue-green
Reticulocytes - blue, although reticulum not evident unless staining time is
prolonged.
Method found in "Staining Procedures" George Clark, 4th ed. 
Hope it works - never tried it.
Best of luck
Jacqui 

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu] 
Sent: 26 April 2005 18:01
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 17, Issue 38

Send Histonet mailing list submissions to
	histonet <@t> lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request <@t> lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner <@t> lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."


Today's Topics:

   1. Heinz Body Stain (krat18 <@t> aol.com)
   2. Re: Lyme Disease (Richard Cartun)


----------------------------------------------------------------------

Message: 1
Date: Tue, 26 Apr 2005 12:45:33 -0400
From: krat18 <@t> aol.com
Subject: [Histonet] Heinz Body Stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C718967FC22B1B-F98-C0F1 <@t> FWM-D40.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Our Hematology dept. asked us to develop a procedure for Heinz Body Stain.
Does anyone have such a procedure that they could send us?   Thanks for your
help!
 
Karen_Raterman <@t> ssmhc.com


------------------------------

Message: 2
Date: Tue, 26 Apr 2005 12:46:36 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Lyme Disease
To: <laurie.colbert <@t> huntingtonhospital.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s26e3840.082 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

I have IHC for Borrelia burgdorferi, but I have never seen a positive human
case.  I have obtained positive staining in animal tissue and, obviously,
our positive control tissue (lung injected with B.
burgdorferi).

Richard

Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com> 04/26/05
12:02PM >>>
Will a warthin starry diagnose lyme disease?  Is there an IHC stain that
will stain for this?

Laurie Colbert

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-----------------------------------------------
Confidentiality Notice

This e-mail message, including any attachments, is for the sole use of the
intended recipient(s) and may contain confidential or proprietary
information which is legally privileged.  Any unauthorized review, use,
disclosure, or distribution is prohibited.  If you are not the intended
recipient, please promptly contact the sender by reply e-mail and destroy
all copies of the original message.



------------------------------

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

End of Histonet Digest, Vol 17, Issue 38
****************************************


DISCLAIMER: This e-mail is confidential and privileged. If you are not the
intended recipient please accept our apologies; please do not disclose, copy
or distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful. Please
inform postmaster <@t> rli.mbht.nhs.uk that this message has gone astray before
deleting it.  Comments or opinions expressed in this email are those of
their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.