[Histonet] RE: Histonet Digest, Vol 17, Issue 38 - Heinz body stain

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Wed Apr 27 04:44:08 CDT 2005

I have a Heinz body stain for you:
Rhodinile blue (Simpson, Carlisle, Mallard 1970)
Working solution:
0.5g rhodinile blue (E. Gurr, Michrome No 1156) to 100ml of a 1% solution of
aqueous NaCl. Shake well and filter through No 1 Whatman filter paper.
To a few drops of heparinised or EDTA-treated blood in a small vial, add the
same amount of working solution of stain.
Swirl gently and stand for 2 mins (the stain - not you!!).
Prepare thin smears and air dry.
Examine as usual or mount in resin mountant.
Heinz bodies - deep purple
Erythrocytes - yellow-orange to blue-green
Reticulocytes - blue, although reticulum not evident unless staining time is
Method found in "Staining Procedures" George Clark, 4th ed. 
Hope it works - never tried it.
Best of luck

-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu] 
Sent: 26 April 2005 18:01
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 17, Issue 38

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Today's Topics:

   1. Heinz Body Stain (krat18 <@t> aol.com)
   2. Re: Lyme Disease (Richard Cartun)


Message: 1
Date: Tue, 26 Apr 2005 12:45:33 -0400
From: krat18 <@t> aol.com
Subject: [Histonet] Heinz Body Stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C718967FC22B1B-F98-C0F1 <@t> FWM-D40.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Our Hematology dept. asked us to develop a procedure for Heinz Body Stain.
Does anyone have such a procedure that they could send us?   Thanks for your
Karen_Raterman <@t> ssmhc.com


Message: 2
Date: Tue, 26 Apr 2005 12:46:36 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Lyme Disease
To: <laurie.colbert <@t> huntingtonhospital.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s26e3840.082 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII

I have IHC for Borrelia burgdorferi, but I have never seen a positive human
case.  I have obtained positive staining in animal tissue and, obviously,
our positive control tissue (lung injected with B.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com> 04/26/05
12:02PM >>>
Will a warthin starry diagnose lyme disease?  Is there an IHC stain that
will stain for this?

Laurie Colbert

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