[Histonet] coating slides for paraffin sections of CNS tissue

[Due, Brice]

Hello Julia, I work in a clinical neuropathology lab. We do impox, silver
stains, etc. on both charged (superfrost) and uncharged slides. We also do giant
sctions up to 4in. x 6 in. on plain (uncharged) glass slides. We rarely have
adhesion problems. You must make absolutely sure the sections are fully dried
before you melt and deparaffinize them. If you melt the paraffin too soon, a
water film will become trapped and the sections will begin lifting in xylene.
The best way is to leave the slides in a 37C oven overnight. Second best is room
temp overnight. In my experience, the second most important factor in adhesion
is section flatness. Wrinkles will not only fill up with water, but they will
also not make contact with the slide. In some autopsy cases we also use STA-ON
(chromated gelatin) adhesive, either in the water bath, or smeared on the slide,
but the drying rules above are still mandatory. If overnight is too long for
you, first try it, and then try cutting back the drying times until your
problems reappear.

Good luck,
-brice
Neuropathology Lab
Brigham & Women's Hospital
Boston

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Favara,
Cynthia (NIH/NIAID)
Sent: Tuesday, April 26, 2005 9:06 AM
To: 'Julia Edgar'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] coating slides for paraffin sections of CNS
tissue


Julia,

I work with primarily rodent CNS tissue and use Superfrost/Plus I get them
from Fischer in the US Cat# 22-034-979 and very rarely have any tissue loss
generally when I neglect to properly dry the slide. Hope this is helpful.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Julia Edgar [mailto:je22r <@t> udcf.gla.ac.uk] 
Sent: Tuesday, April 26, 2005 1:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] coating slides for paraffin sections of CNS tissue

Dear All
We've been using APES coated slides for collecting paraffin embedded CNS
tissue for immunohistochemistry (with microwave treatment) but the sections
are becoming unstuck, to some extent, during processing. I will be grateful
if you can tell me what you are coating slides with for small pieces of CNS
tissue.
Thank you
Julia
Julia Edgar BSc (Hons), PhD
University of Glasgow
Tel: 0141 330 5818
e-mail: je22r <@t> udcf.gla.ac.uk


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