[Histonet] slide dryer

Wed Apr 20 12:47:24 CDT 2005

MOPEC has a nice one that is a little bit larger and spins the racks while drying.  Thermo has a couple of new ones that I haven't tried yet.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533

My opinions are my own and do not reflect those of my employer.  Long live free speech!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Clarke, Mary
Sent: Wednesday, April 20, 2005 10:47 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] slide dryer

I have an old slide dryer from Lipshaw that is dying and wondering if anyone knows where I can get a replacement.  It measures about 10x7x5 inches.  The model number is 218.  Thanks in advance for your help.

Terri Clarke
Histology Supervisor
All Saints Laboratory
3801 Spring Street
Racine, Wi 53405
mclarke <@t> allsaintshealthcare.org
262-687-6609

-----Original Message-----
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Sent: Tuesday, April 19, 2005 12:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 17, Issue 31

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Today's Topics:

   1. Re: Methyl green pyronin staining, methyl green source
      (John A. Kiernan)
   2. EDTA (Trisha Emry)
   3. snout (Trisha Emry)
   4. IHC fixation (Steven Coakley)
   5. Re: EDTA (Gayle Callis)
   6. Re: Microwave Processors (Robyn Vazquez)
   7. RE: (no subject) (Jennifer MacDonald)
   8. Re: neutral stains for protozoa (Jennifer MacDonald)
   9. RE: neutral stains for protozoa (Yaskovich, Ruth A (NIH/NIDCR))
  10. Paraffin processing late stage mouse embryos (Johnson, Teri)
  11. RE: Cryostat (Charles  Scouten)
  12. New Histology and MOH's Technologist Job Opportunities
      (Matt Hawes (extension 224))
  13. Qc forms (Steven Coakley)
  14. QC & QA's (Flores, Teresa)
  15. RE: neutral stains for protozoa (Patsy Ruegg)

----------------------------------------------------------------------

Message: 1
Date: Mon, 18 Apr 2005 13:15:31 -0400
From: "John A. Kiernan" <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Methyl green pyronin staining, methyl green
	source
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4263EB33.7494C303 <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii

The dye sold by Sigma is not methyl green. It is
ethyl green, which is better. They call it methyl
green on the label, but with the correct CI number
(the one for ethyl green). Ethyl green can be found
also under its right name in the Sigma catalog, with 
a cross-reference to the methyl green entry (which has 
ethyl green as a synonym, and the correct molecular
formula for ethyl green). I don't know why they
go on calling their ethyl green methyl green. The
latter is a dye nobody would want these days because 
of its inevitable contamination with crystal violet. 
The CV originates during manufacture of MG and is 
also produced by spontaneous degradation in the stored
dye powder. Ethyl green is made in a different way
and it is also much more stable than methyl green.

If anyone sells real methyl green, it must be from 
very old stock because that dye has not been
manufactured for many (?30-40) years. See Floyd Green: 
Sigma-Aldrich Handbook of Stains, Dyes and 
Indicators (1991) for explanation of why ethyl
green in not contaminated and does not change
slowly into crystal violet. The differences between
the two dyes are explained also in both the 9th 
and the 10th editions of Conn's Biological Stains.

The staining method for DNA & RNA has, in reality,
been ethyl green-pyronine for many years. Recognition
of this fact in staining manuals and catalogs is 
long overdue. A standardized EGP method (Hoyer et al
1986 Histochem. J. 18:90-94) is technically simpler 
than MGP procedures that date from the days of real
methyl green.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Gayle Callis wrote:
> 
> Following the thread here.
> 
> One can purchase methyl violet free Methyl reen from Sigma.  This avoids
> the messy procedure to remove methyl violet using toxic chloroform and a
> separating funnel inside a fume hood. We changed to Sigmas MG instead of
> taking a chance other companies methyl green dye lots were not MV free.
> 
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 2
Date: Mon, 18 Apr 2005 22:35:43 -0700
From: "Trisha Emry" <emry <@t> u.washington.edu>
Subject: [Histonet] EDTA
To: "histo" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <NDBBIBEPALLAJHEDNELICEEFCBAA.emry <@t> u.washington.edu>
Content-Type: text/plain;	charset="iso-8859-1"

I can buy two kinds of EDTA through my local university stores.

One can be used for electrophoresis (Fisher BP 120-500 $27.38)
and the other just listed as powder (Fisher S311-500 $36.37).

With the great price difference (we have been using the $36.37 type) I was
wondering if someone could tell me if there really is a difference and if I
have to continue to use the pricey type.

Thanks,
Trisha
U of Washington, Seattle

------------------------------

Message: 3
Date: Mon, 18 Apr 2005 22:40:39 -0700
From: "Trisha Emry" <emry <@t> u.washington.edu>
Subject: [Histonet] snout
To: "Histonet <@t> lists. utsouthwestern. edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <NDBBIBEPALLAJHEDNELIMEEFCBAA.emry <@t> u.washington.edu>
Content-Type: text/plain;	charset="iso-8859-1"

We are decalcifying large 4x4x1 or 2 inch pieces of pig snout and the limits
of my microtome make it impossible for me to cut.

Are there any contract labs out there that can do the sectioning when we get
them decalcified?

Thanks,

Trisha
U of Washingtion, Seattle

------------------------------

Message: 4
Date: Mon, 18 Apr 2005 13:23:30 -0500
From: "Steven Coakley" <steven.coakley <@t> mirusbio.com>
Subject: [Histonet] IHC fixation
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <auto-000001470719 <@t> mirusbio.com>
Content-Type: text/plain;	charset="us-ascii"

Inquiring about formalin substitutes for IHC in order to eliminate or
decrease antigen retrieval.

Steven

------------------------------

Message: 5
Date: Mon, 18 Apr 2005 12:33:13 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] EDTA
To: "Trisha Emry" <emry <@t> u.washington.edu>,
	Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20050418120752.01b4b1b0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

E sure to know which EDTA you are working with, there are several.

EDTA disodium salt, it will work for decalcification and if only soluble at 
~ 10 g/100 ml water with pH of 4 to 6 (5% solution)

EDTA (no sodiums added) will work also.  It is only soluble at ~10g/100 ml 
water, and will have pH of approx 4 to 6 (5% solution).

EDTA tetrasodium salt is very soluble in buffers/water, as much as 14g/100 
mls, but is very alkaline at pH 9, you must adjust the pH down and Webb Jee 
(very famous bone person) used acetic acid.  This is the EDTA we prefer for 
bone work.

Fisher catalog show no great differences between these two different grades 
of EDTA.

(Fisher BP 120-500 $27.38) is electrophoresis grade, has been tested for 
lots of different things, found under Bioreagent listings and is the 
disodium salt.

(Fisher S311-500 $36.37) is ACS certified found in analytical section of 
their catalog, extensively tested, and is the a disodium salt.

It helps to keep a Fisher chemical catalog around to check out chemical 
purity, etc, etc.

Either will work, go for the cheapest.    If all you are doing is 
decalcification, then technical grade, even cheaper works just as well, the 
tetrasodium salt is technical grade.

At 11:35 PM 4/18/2005, you wrote:
>I can buy two kinds of EDTA through my local university stores.
>
>One can be used for electrophoresis (Fisher BP 120-500 $27.38)
>and the other just listed as powder (Fisher S311-500 $36.37).
>
>With the great price difference (we have been using the $36.37 type) I was
>wondering if someone could tell me if there really is a difference and if I
>have to continue to use the pricey type.
>
>Thanks,
>Trisha
>U of Washington, Seattle
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

------------------------------

Message: 6
Date: Mon, 18 Apr 2005 11:34:46 -0700
From: "Robyn Vazquez" <vazquezr <@t> ohsu.edu>
Subject: Re: [Histonet] Microwave Processors
To: conniegrubaugh <@t> hotmail.com,	histonet <@t> lists.utsouthwestern.edu
Message-ID: <s2639b69.099 <@t> OHSU.EDU>
Content-Type: text/plain; charset=us-ascii

Connie,
Make sure it is a complete tissue processing microwave.  We have a Ted Pella, which would be great except it doesn't have water loader and therefore it doesn't process properly.  My department isn't willing to buy it.  We are moving and will purchasing a tissue processor.

Robyn
OHSU

------------------------------

Message: 7
Date: Mon, 18 Apr 2005 11:37:30 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: RE: [Histonet] (no subject)
To: "Kristen Broomall" <kbroomal <@t> NEMOURS.ORG>
Cc: 'Histonet' <Histonet <@t> lists.utsouthwestern.edu>,	'Shawn Leslie'
	<sleslie <@t> northernhealth.org>,
	histonet-bounces <@t> lists.utsouthwestern.edu,	'Shirley Powell'
	<POWELL_SA <@t> Mercer.edu>,	'Gareth Davis' <mrsgbd2001 <@t> yahoo.com>
Message-ID:
	<OF220DC565.DA17A2E6-ON88256FE7.00663B7E-88256FE7.00671849 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Beginning in 2001, the ASCP added cytology preparation and staining to the 
HT exam.

"Kristen Broomall" <kbroomal <@t> NEMOURS.ORG> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/12/2005 02:20 PM

To
"'Shirley Powell'" <POWELL_SA <@t> Mercer.edu>, "'Gareth Davis'" 
<mrsgbd2001 <@t> yahoo.com>, "'Shawn Leslie'" <sleslie <@t> northernhealth.org>, 
"'Histonet'" <Histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] (no subject)

I can attest to that too. 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Shirley
Powell
Sent: Tuesday, April 12, 2005 5:16 PM
To: 'Gareth Davis'; 'Shawn Leslie'; 'Histonet'
Subject: RE: [Histonet] (no subject)

A histotech I know took the HT last weekend here and told me there was a 
lot
more cytology than expected on hers. 

Shirley Powell 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gareth 
Davis
Sent: Tuesday, April 12, 2005 3:50 PM
To: Shawn Leslie; Histonet
Subject: Re: [Histonet] (no subject)

Hi,
I took the HT computer test last December.  One helpful hint I can give is
to know everything about tissue idenitification, what stain is needed for
that tissue, what may go wrong with stain for a specific tissue and how to
identify mistakes.  I saw so many images, and it really caught me 
offguard.
I expected many, but not as many as I saw and the questions to the images
were not what I expected.  I did pass with a good score, but I was really
surprised that I did. 
Good luck.

Gareth Davis

--- Shawn Leslie <sleslie <@t> northernhealth.org> wrote:
>                <mailto:histonet <@t> pathology.swmed.edu>
> 
> To All,
> 
> I have a trainee who is about to sit for her HT computer exam, and 
> wondered if anyone has taken it recently, and could offer some helpful 
> hints. Thanks
> 
> Shawn Leslie
> 
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------------------------------

Message: 8
Date: Mon, 18 Apr 2005 12:18:08 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] neutral stains for protozoa
To: Gary Radice <gradice <@t> richmond.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<OFAF560CF8.4F227967-ON88256FE7.0069F723-88256FE7.006AD085 <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

There is an iron hematoxylin procedure for protozoa.  It is in the AFIP 
manual.  I don't have it.  It is Heidens or something similar.

Gary Radice <gradice <@t> richmond.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/31/2005 06:47 AM

To
Histonet <@t> lists.utsouthwestern.edu
cc

Subject
[Histonet] neutral stains for protozoa

I have a colleague who is studying a calcified protozoan. She is 
specifically interested in calcification, and she is looking for stains 
she can use that are not acidic and that don't require acid 
differentiation steps, so that she doesn't inadvertently decalcify the 
specimens.

Any thoughts about nuclear or counterstains that might work in this 
situation?

Gary P. Radice gradice <@t> richmond.edu
Department of Biology            804-289-8107 (voice)
University of Richmond           804-289-8233 (FAX)
Richmond VA 23173                http://www.richmond.edu/~gradice

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Mon, 18 Apr 2005 15:27:32 -0400
From: "Yaskovich, Ruth A (NIH/NIDCR)" <ryaskovich <@t> dir.nidcr.nih.gov>
Subject: RE: [Histonet] neutral stains for protozoa
To: Gary Radice <gradice <@t> richmond.edu>, 'Jennifer MacDonald'
	<JMacDonald <@t> mtsac.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
	<8F3AB322628548428A992EFB0E80F5D315D630D6 <@t> nihexchange8.nih.gov>
Content-Type: text/plain;	charset="iso-8859-1"

You can stain with the Von Kossa with a nuclear fast red counterstain.
Ruth Yaskovich
N.I.H.

> ----------
> From: 	Jennifer MacDonald
> Sent: 	Monday, April 18, 2005 2:18 PM
> To: 	Gary Radice
> Cc: 	Histonet <@t> lists.utsouthwestern.edu;
> histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] neutral stains for protozoa
> 
> There is an iron hematoxylin procedure for protozoa.  It is in the AFIP 
> manual.  I don't have it.  It is Heidens or something similar.
> 
> 
> 
> 
> 
> 
> Gary Radice <gradice <@t> richmond.edu> 
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 03/31/2005 06:47 AM
> 
> To
> Histonet <@t> lists.utsouthwestern.edu
> cc
> 
> Subject
> [Histonet] neutral stains for protozoa
> 
> 
> 
> 
> 
> 
> I have a colleague who is studying a calcified protozoan. She is 
> specifically interested in calcification, and she is looking for stains 
> she can use that are not acidic and that don't require acid 
> differentiation steps, so that she doesn't inadvertently decalcify the 
> specimens.
> 
> Any thoughts about nuclear or counterstains that might work in this 
> situation?
> 
> 
> 
> Gary P. Radice gradice <@t> richmond.edu
> Department of Biology            804-289-8107 (voice)
> University of Richmond           804-289-8233 (FAX)
> Richmond VA 23173                http://www.richmond.edu/~gradice
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

------------------------------

Message: 10
Date: Mon, 18 Apr 2005 14:48:00 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Paraffin processing late stage mouse embryos
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1113930000.28848.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

Is anybody doing paraffin processing on E18.5 - P0 mouse embryos that
can help us optimize our in-house protocol?  I know that when possible
one should bisect embryos for best fixation and processing.
Unfortunately we can't always do that.  In some instances, we are able
to bisect the samples just posterior to the forelimbs and process only
the top 1/2 of the embryo.  Even after poking some holes and immersion
for 72 hours in 10% NBF, combined with a rigorous processing schedule (2
hours/station with a typical automated tissue processor setup--same
schedule as we use for decalcified whole adult mouse heads!), our
samples just aren't sectioning well, and they tend to blow up on the
water bath.  A test with samples without holes poked in them vs. ones
with holes produced no discernable improvement in processing.

A google search turned up one lab (evidently hand processing) who
recommends removing the skin of this size embryo (E15.5 - E18.5)  and
removing the head and/or limbs if not needed for study.  Fixation and
processing: fix 1 hour @ 40 degrees C, change fixative, then 40 degrees
C overnight. The following are done at room temp or refrigerated for IHC
or ISH: dehydrating in 30% ETOH 1-2 hrs, 50% ETOH 1-2 hrs, 70% ETOH 4
hrs or O/N, 95% ETOH 3 hrs x 2 or O/N, and 100% ETOH 1 hr x 2.  Embryos
can be stored at -20 degrees C at this point.  Carrying on with
dehydration and infiltration: 100% ETOH 1 hr x 3 @ RT, Xylene 30-40 min
x 2 (check embryos*), paraffin 40 minutes x 3, paraffin embedding.

*No indication of what we're actually checking on these samples

I do think that removing the skin on these guys would help immensely.  I
also think that additional dissection when possible is critical to good
fixation and processing.

Any comments on the above processing schedule?  Is anybody doing these
later stage mouse embryo samples using a protocol that's working for
you?

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org

------------------------------

Message: 11
Date: Mon, 18 Apr 2005 15:13:49 -0500
From: "Charles  Scouten" <cwscouten <@t> myneurolab.com>
Subject: RE: [Histonet] Cryostat
To: "Chung, Luong" <lchung <@t> ppmh.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<5784D843593D874C93E9BADCB87342AB44F87C <@t> tpiserver03.Coretech-holdings.com>

Content-Type: text/plain;	charset="iso-8859-1"

See the following link:  This works great.

http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=475102&catdesc=Histology+Equipment&CatThreeID=732&CatOneID=4&subcatdesc=Cryostats&idsubcategory=182

Cordially,
Charles W.  Scouten, Ph.D. 
myNeuroLab.com 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 
http://www.myneurolab.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chung, Luong
Sent: Monday, April 18, 2005 8:14 AM
To: Histonet (E-mail)
Subject: [Histonet] Cryostat

All,

I'm looking for a reliable cryostat with self disinfection.  Any suggestion?

Thanks

Bruce Chung, MSM, HT/CT(ASCP)
Anatomic Pathology Manager
Phoebe Putney Memorial Hospital
lchung <@t> ppmh.org

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------------------------------

Message: 12
Date: Mon, 18 Apr 2005 17:03:51 -0400
From: Matt Hawes (extension 224) <matt <@t> ategra.com>
Subject: [Histonet] New Histology and MOH's Technologist Job
	Opportunities
To: Histonetters <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <QjM1SEtaVCYoMTk3TC5gMTAwNDI2MDc4 <@t> bigdell3>
Content-Type: text/plain

Hello Histonetters, 

My name is Matt Hawes and I am the Senior AP Recruiter here at Ategra Systems and I have received  new Histology and MOH's  Technologist Job Opportunities that I wanted to inform you about and see if you might be interested or if you might happen to know of anyone looking for new employment opportunities?

Here are some of my HOTTEST Fulltime Permanent Histo Tech positions & MOH's Tech opportunities:

1. Boston Burbs, MA-  MOH's/ Histo Tech- Day Shift
2. Rockville, MD- MOH's/Histo Tech- Day Shift
3. Northeastern MI- Histo Tech-Day Shift
4. Sioux City, IA- Histo Tech-Day Shift
5. Los Angeles, CA- IHC/Hist Supervisor
6. Rhode Island- Histo Tech-Day Shift
7. New Jersey-Histo Tech- Nite Shift (3 openings)
8. Upstate NY- Histo Tech- Day Shift (2 openings)

These positions are as direct employees of our clients. They all offer full employee benefits (if working in a fulltime capacity): excellent healthcare, life insurance, dental, vision, 401K/retirement - as well as relocation bonuses (where applic).

If you are interested in these jobs, please CALL ME ASAP at (800)466 9919 x224 or e-mail me at Matt <@t> ategra.com . To speed things up, please also send me a copy of your resume, (if you haven't already done so). If you are interested in jobs outside the above-mentioned areas, please send me your resume as well as I have clients throughout the US. I will keep your resume confidential and will not release it to anyone without your permission (This is Ategra policy as well as my own). My services are at no charge to you.

Of course, you may be happy in your present job, but it never hurts to to keep an eye open. Also, if you have friends/peers who might be interested as well, if you could pass my query & name on to them I'd be very grateful. 

However, if you are interested in any of the jobs above, please call me. 

Thank You !!

Matt Hawes
Senior Laboratory Recruiter
Ategra Systems
(800) 466-9919 Ext 234
Matt <@t> Ategra.com
---------------------------------------------------------
Ategra Systems Inc
Specialists in Permanent & Contract Staffing

VOICE: 407-671-5800 ext 224
TOLLFREE: 800-466-9919 Ext 224

Note: this message is intended for: 
Histonetters at histonet <@t> lists.utsouthwestern.edu

To Learn More About Ategra:
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------------------------------

Message: 13
Date: Tue, 19 Apr 2005 08:41:41 -0500
From: "Steven Coakley" <steven.coakley <@t> mirusbio.com>
Subject: [Histonet] Qc forms
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <auto-000001471569 <@t> mirusbio.com>
Content-Type: text/plain;	charset="us-ascii"

I am starting up a histo lab and in need of varies qc forms.  I got a few
from Carsons.  Any would be appreciated.
Email me at sjchtascp <@t> yahoo.com

Thanks

------------------------------

Message: 14
Date: Tue, 19 Apr 2005 08:49:03 -0500
From: "Flores, Teresa" <tflore <@t> lsuhsc.edu>
Subject: [Histonet] QC & QA's
To: histonet <@t> lists.utsouthwestern.edu
Cc: histo <@t> nsh.org
Message-ID:
	<FFBCB2526662D411A17600B0D03D55F01308FE51 <@t> lsumc-med-exch2.med.lsuhsc.edu>

Content-Type: text/plain

I know I have seen them, but is there a site for Histotechnology QC and QA
on sectioning techniques, staining techniques and quality, etc?
Teresa Flores
LSUHSC
NO, LA

------------------------------

Message: 15
Date: Tue, 19 Apr 2005 10:56:21 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] neutral stains for protozoa
To: "'Yaskovich, Ruth A \(NIH/NIDCR\)'"
	<ryaskovich <@t> dir.nidcr.nih.gov>,	"'Gary Radice'"
	<gradice <@t> richmond.edu>,	"'Jennifer MacDonald'" <JMacDonald <@t> mtsac.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu,
	histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <200504191656.j3JGuCOD021472 <@t> chip.viawest.net>
Content-Type: text/plain;	charset="US-ASCII"

I use a H&E counterstain for Von Kossa.  I use gills 3 hematoxylin and do
not destain in acid alcohol/ I do blue briefly in ammonia water, wash well
and then use a 0.2% aqueous eosin for 30 min., dehydrate quickly thru 95%
alcohols, 100 and xylene then permount.  This method will stain osteoid on
bone viewed by fluorescence UV.  It is very nice for histomorphometry by
image analysis as only the osteoid lights up and the rest of the tissue
(calcified bone and blue nuclei) is dark under UV, makes it really easy to
measure osteoid seams.
Patsy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yaskovich,
Ruth A (NIH/NIDCR)
Sent: Monday, April 18, 2005 12:28 PM
To: Gary Radice; 'Jennifer MacDonald'
Cc: Histonet <@t> lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] neutral stains for protozoa

You can stain with the Von Kossa with a nuclear fast red counterstain.
Ruth Yaskovich
N.I.H.

> ----------
> From: 	Jennifer MacDonald
> Sent: 	Monday, April 18, 2005 2:18 PM
> To: 	Gary Radice
> Cc: 	Histonet <@t> lists.utsouthwestern.edu;
> histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] neutral stains for protozoa
> 
> There is an iron hematoxylin procedure for protozoa.  It is in the 
> AFIP manual.  I don't have it.  It is Heidens or something similar.
> 
> 
> 
> 
> 
> 
> Gary Radice <gradice <@t> richmond.edu>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 03/31/2005 06:47 AM
> 
> To
> Histonet <@t> lists.utsouthwestern.edu
> cc
> 
> Subject
> [Histonet] neutral stains for protozoa
> 
> 
> 
> 
> 
> 
> I have a colleague who is studying a calcified protozoan. She is 
> specifically interested in calcification, and she is looking for 
> stains she can use that are not acidic and that don't require acid 
> differentiation steps, so that she doesn't inadvertently decalcify the 
> specimens.
> 
> Any thoughts about nuclear or counterstains that might work in this 
> situation?
> 
> 
> 
> Gary P. Radice gradice <@t> richmond.edu
> Department of Biology            804-289-8107 (voice)
> University of Richmond           804-289-8233 (FAX)
> Richmond VA 23173                http://www.richmond.edu/~gradice
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
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> 
> 

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End of Histonet Digest, Vol 17, Issue 31
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