[Histonet] RE: CYTOLOGY PREP BY HISTOLOGY
Hopkins, Karen
KMH.02 <@t> ex.uchs.org
Fri Apr 15 10:42:20 CDT 2005
The Histology department at our Hospital is responsible for prepping all cytology specimens. I am interested in information about methods that other facilities use to prep cytology specimens. Generally, we accept direct smears for GYN specimens and send thinprep gyn's out. For non-gyn specimens we do a combination of air-dryed smears, direct smears, thinprep slides, and cytospin slides (Shandon 4 cytospin). We centrifuge many of the specimens and also vortex some. CB's we basically just centrifuge with CytoLyt solution and submit the cell button.
I am curious about the following: Filtration methods including cyto-shuttle filtration, Plasma-Thrombin and cytoblock methods for cellblocks, and Autocyte Prep for thin layer preparation systems. Also, if anyone has any knowledge comparing thinprep to Surepath for gyn's I be interested in your comments.
I'm working on something for continuing education and want to include the methods I am not familiar with if they are being utilized today. Thanks in advance for any and all responses. Karen
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, April 15, 2005 11:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 17, Issue 25
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Today's Topics:
1. Re: EDTA --> pure water (John Kiernan)
2. Incredible book on mouse anatomy (Gayle Callis)
3. Re: Her2 testing (Promila Rastogi)
4. Source for adjustable workstations? (Tim Webster)
5. RE: Source for adjustable workstations? (Kristen Broomall)
6. Re: Tween charge (Jennifer MacDonald)
7. Source for adjustable workstations? (David Haagsma)
8. "Vision Biosystems is now being distributed in the US"
(saya narra)
9. RE: Source for adjustable workstations? (Larry Woody)
10. Timer Search (Mitchell Jean A.)
11. Microm IHC stainer (Carol Bobrowitz)
12. RE: Processors (Bill Sinai)
13. RE: Long and short biopsy runs with one processor (Bill Sinai)
14. Distributor (saya narra)
15. Re: Histonet Posting - Tampa Area (JMyers1 <@t> aol.com)
16. RNA in situ hybridization-staining problems (Schmitz, Nicole)
17. Re: RNA in situ hybridization-staining problems (- -)
18. RCC Antibody Help (Sharon Chase)
19. RE: RCC Antibody Help (Dawson, Glen)
20. Temp/Humidity Records? (Breeden, Sara)
21. RE: Temp/Humidity Records?
(Marshall Terry Dr, Consultant Histopathologist)
22. RE: Temp/Humidity Records? (Pamela A. Marcum)
23. RE: Long and short biopsy runs with one processor
(Charles Scouten)
24. CD31 on Mouse (jo-ann-e.bader)
25. RE: CD31 on Mouse (Elizabeth Chlipala)
26. RE: Temp/Humidity Records? (Charles, Roger)
----------------------------------------------------------------------
Message: 1
Date: Thu, 14 Apr 2005 12:58:24 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] EDTA --> pure water
To: Trisha Emry <emry <@t> u.washington.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <425EA130.CBBBA9BC <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii
When instructions specify "water," without qualification,
it is usual to use purified water. If tap water (whatever
might be dissolved in it) is OK, the instructions will
usually say "tap water" explicitly.
EDTA has various uses; in histological work they mostly
involve removal of calcium ions. If your tap water is
hard (= contains calcium ions), it will use up some of
the EDTA in the solution. If you know the Ca content of
the tap water you can calculate the equivalent amount
of wasted EDTA. One mole of calcium ions (40 grams)
combines with one mole of disodium EDTA dihydrate (372
grams).
For example, if a litre of tap water contains 100mg of
calcium, that will consume 100X372/40 =930mg of EDTA.
This would reduce the available EDTA in a 1% solution to
about 0.9%. I do not know if my guess of 100ppm Ca is
anywhere near reasonable for hard water, or if a
certificate of tap water analysis reports the calcium
content in this way. If my guesstimate is too low,
hard water may eat up more than 10% of the EDTA in
a 1% solution.
A simple test for minimally "clean" lab water is to mix
it with an equal volume of 1% aqueous silver nitrate.
Any precipitate or opalescence indicates that the
water contains anions with insoluble Ag salts or
organic compounds that reduce Ag+.
John Kiernan
Anatomy, UWO,
London, Canada.
_______________________________________
Trisha Emry wrote:
>
> Is it necessary to use distilled H20 for EDTA or can you use tap?
>
> Is anyone using heat with EDTA? Please send details.
>
> Thank you,
>
> Trisha Emry
> U of WA, Seattle
________________________________
------------------------------
Message: 2
Date: Thu, 14 Apr 2005 11:49:30 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Incredible book on mouse anatomy
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050414114731.01b06fd0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
A Color Atlas of Sectional Anatomy of the Mouse
by Takamasa Iwaki, Hiroshi Yamashita, Toshiyuki Hayakawa
Published in Japan in 15th September 2001
185 Pages Type: hardcover
It costs $175.00, but is filled with spectacular color photos, text is in
English and Japanese,
Go to Braintree Scientific Website
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 3
Date: Thu, 14 Apr 2005 11:08:30 -0700
From: "Promila Rastogi" <p.rastogi <@t> biogenex.com>
Subject: Re: [Histonet] Her2 testing
To: <histonet <@t> lists.utsouthwestern.edu>, "Cindy DuBois"
<dpahisto <@t> yahoo.com>
Message-ID:
<37DC9F93CF7F864182D0463EF93D571B034B40 <@t> ISLETON2.california.biogenex.com>
Content-Type: text/plain; charset="us-ascii"
FYI, BioGenex recently received FDA approval for our InSite HER2/neu
kit.
Promila Rastogi
Reagents Product Manager
>Message: 17
>Date: Thu, 14 Apr 2005 09:19:51 -0700 (PDT)
>From: Cindy DuBois <dpahisto <@t> yahoo.com>
>Subject: [Histonet] Her2 testing
>To: Histonet <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <20050414161951.64144.qmail <@t> web32107.mail.mud.yahoo.com>
>Content-Type: text/plain; charset=us-ascii
>Is it still true that only the Dako Her2 is FDA approved?
>If not, what everyone's preference for this antibdody?
>Cindy DuBois
------------------------------
Message: 4
Date: Thu, 14 Apr 2005 14:17:22 -0400
From: "Tim Webster" <twebster <@t> nmcinc.org>
Subject: [Histonet] Source for adjustable workstations?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DB413B5947B2094597E03C57E12DC8E307DF65 <@t> exchange.nmcinc.org>
Content-Type: text/plain; charset="US-ASCII"
Hi All,
A recent ergonomic assessment of our Histo Lab produced a seven page litany
of ergonomic ills (Yay!) As we are about to renovate in stages, I was
looking for a source of adjustable tables/benches/stations for embedding and
microtomy. Staff height varies from 5'2" to 6'1+3/4.
Adjusting the height of the chair is inadequate for the height of the benches
and equipment. I have one supplier of adjustable tables
(Custom-products.com) I would love someone to offer another name. (I
struggle finding stuff on the web sometimes . . .) Also, how do you folks
handle ergonomics for routine histo?
Have a great day
Tim
Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street
St Albans, VT 05478
(802) 524-1070
twebster <@t> nmcinc.org <mailto:twebster <@t> nmcinc.org>
------------------------------
Message: 5
Date: Thu, 14 Apr 2005 14:29:44 -0400
From: Kristen Broomall <kbroomal <@t> NEMOURS.ORG>
Subject: RE: [Histonet] Source for adjustable workstations?
To: 'Tim Webster' <twebster <@t> nmcinc.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<9BCAC308B27CAD4196D8AC9ADF037AAA110A79B5 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset="iso-8859-1"
"Also, how do you folks handle ergonomics for routine histo?"
Ibuprofen and muscle relaxers.
Sorry, I couldn't resist. (I'm not making it up though!) But seriously, I'm
5'2" & get horrible muscle spasms in my shoulder from sitting at the
microtome for long periods at a time, no matter how high the chair is
positioned. I must stretch my body in some funky direction to reach the
wheel. I've gotten used to using the automated part of the microtome with
the foot pedal.
I'd love to hear what you come up with!
Kristen Broomall
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Tim
Webster
Sent: Thursday, April 14, 2005 2:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Source for adjustable workstations?
Hi All,
A recent ergonomic assessment of our Histo Lab produced a seven page litany
of ergonomic ills (Yay!) As we are about to renovate in stages, I was
looking for a source of adjustable tables/benches/stations for embedding and
microtomy. Staff height varies from 5'2" to 6'1+3/4.
Adjusting the height of the chair is inadequate for the height of the
benches
and equipment. I have one supplier of adjustable tables
(Custom-products.com) I would love someone to offer another name. (I
struggle finding stuff on the web sometimes . . .) Also, how do you folks
handle ergonomics for routine histo?
Have a great day
Tim
Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street
St Albans, VT 05478
(802) 524-1070
twebster <@t> nmcinc.org <mailto:twebster <@t> nmcinc.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Thu, 14 Apr 2005 11:34:28 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: Re: [Histonet] Tween charge
To: Dorothy.L.Webb <@t> HealthPartners.Com
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFA2337312.8FEC43C2-ON88256FE3.0065FB2C-88256FE3.0066D1FE <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"
I think this would fall under the category of soaking, like overdehydrated
blocks. There is no charge for this.
Dorothy.L.Webb <@t> HealthPartners.Com
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
04/14/2005 07:30 AM
To
histonet <@t> lists.utsouthwestern.edu
cc
Subject
[Histonet] Tween charge
Does anyone know of the "charge" that should be used for softening toe or
fingernails to prepare for processing? Could a decal charge be safely
used?
It seems to me that with taking the time and the solutions involved, there
should be some sort of charge incurred. Any thoughts and thanks much!!
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------------------------------
Message: 7
Date: Thu, 14 Apr 2005 14:43:23 -0400
From: "David Haagsma" <DavidH <@t> marketlabinc.com>
Subject: [Histonet] Source for adjustable workstations?
To: <histonet <@t> lists.utsouthwestern.edu>, "Tim Webster"
<twebster <@t> nmcinc.org>
Message-ID:
<19E3602A16438E48B51A4250CA04B5F60F98CC <@t> exchange.marketlab.com>
Content-Type: text/plain; charset="US-ASCII"
Tim,
MarketLab Inc. has adjustable microtome stations,
http://www.marketlabinc.com/products/product.cfm/ML3158
And adjustable height grossing stations
http://www.marketlabinc.com/products/product.cfm/ML3217
http://www.marketlabinc.com/products/categorydetail.cfm/722
And Microtome Arm Rests
http://www.marketlabinc.com/products/product.cfm/ML1038L
Thank you,
Dave Haagsma
www.marketlabinc.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim
Webster
Sent: Thursday, April 14, 2005 2:17 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Source for adjustable workstations?
Hi All,
A recent ergonomic assessment of our Histo Lab produced a seven page
litany
of ergonomic ills (Yay!) As we are about to renovate in stages, I was
looking for a source of adjustable tables/benches/stations for embedding
and
microtomy. Staff height varies from 5'2" to 6'1+3/4.
Adjusting the height of the chair is inadequate for the height of the
benches
and equipment. I have one supplier of adjustable tables
(Custom-products.com) I would love someone to offer another name. (I
struggle finding stuff on the web sometimes . . .) Also, how do you
folks
handle ergonomics for routine histo?
Have a great day
Tim
Tim Webster
Histology Specialist
Northwestern Medical Center
133 Fairfield Street
St Albans, VT 05478
(802) 524-1070
twebster <@t> nmcinc.org <mailto:twebster <@t> nmcinc.org>
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Thu, 14 Apr 2005 11:46:26 -0700 (PDT)
From: saya narra <sayanarra <@t> yahoo.com>
Subject: [Histonet] "Vision Biosystems is now being distributed in the
US"
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050414184626.52381.qmail <@t> web60620.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Rick !
It appears your automated immunos are overpriced
compared to Dako. As a general rule, it's not good to
advertise what isn't competitive in a given market.
Business 101.
Also, you said " Vision Biosystems is now
being distributed in the U.S. ". Who is the
distributor ? Can you get me the Company info so I
may contact them re pricing, service etc. I wasn't
aware that Vision Biosystems had found a
"distributor". What is the website of the Distributor
? Is it Leica ?
Saya Narra
UofT
__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/
------------------------------
Message: 9
Date: Thu, 14 Apr 2005 11:49:58 -0700 (PDT)
From: Larry Woody <slappycraw <@t> yahoo.com>
Subject: RE: [Histonet] Source for adjustable workstations?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050414184959.12724.qmail <@t> web52606.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
I have degenerative disc disease in my neck from 25 yrs of cutting but what may surprise you is that standing up to cut bothers me a whole lot less than sitting down, and it is less pressure on the back to stand up and I use a lot less ibuprofen. Larry
---------------------------------
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
------------------------------
Message: 10
Date: Thu, 14 Apr 2005 14:29:14 -0500
From: "Mitchell Jean A." <ja.mitchell <@t> hosp.wisc.edu>
Subject: [Histonet] Timer Search
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<583D3E9A1E843445BD54E461D1A2F6F30F83F596 <@t> uwhis-xchng2.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
I am on a search for the large (approx 3 1/2 in square) grey winding
(120 minute) timers that were ever so popular in the lab once upon a
time. Anyone have a clue if they are still available anywhere?
I've been searching for awhile & can't come up with a source.
Thanks much,
Jean Mitchell, BS, HT (ASCP)
University of Wisconsin Hospital & Clinics
Department of Neurology, Neuromuscular Laboratory
Madison, WI
------------------------------
Message: 11
Date: Thu, 14 Apr 2005 15:30:39 -0500
From: Carol Bobrowitz <carolb <@t> mail.phys.mcw.edu>
Subject: [Histonet] Microm IHC stainer
To: "'Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <59DF6640CDC0D7119DF000D0B761393C1A965A <@t> thor.phys.mcw.edu>
Content-Type: text/plain; charset="iso-8859-1"
I'm curious.
Any Histology techs out there that have demo'd the Microm IHC stainer?
What are your comments?
If Richard Allan was purchased by Fisher, who does the repairs and is there
a yearly service contract?
Vendors, Please do not send me any information. I would like comments from
users
not a sales pitch.
Thanks,
Carol Ann Bobrowitz
Histology Laboratory
Department of Physiology - Room 541
Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, Wisconsin 53226
414-456-8179
FAX 414-456-6546
------------------------------
Message: 12
Date: Fri, 15 Apr 2005 07:24:48 +1000
From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
Subject: [Histonet] RE: Processors
To: "'saya narra'" <sayanarra <@t> yahoo.com>, "histonet \(E-mail\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001401c54138$632f85c0$0ecd080a <@t> wsahs.nsw.gov.au>
Content-Type: text/plain; charset="US-ASCII"
I believe they are looking for a distributor in the USA. I have heard no
rumour about the company being for sale. Histology equipment is but a small
part of their whole business.
Mt Waverley is in Victoria (the smallest state in Australia) I am in the
outer suburbs of Sydney in NSW. The distance between is 1000klm (700miles)
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: saya narra [mailto:sayanarra <@t> yahoo.com]
Sent: Thursday, 14 April 2005 7:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: bills <@t> icpmr.wsahs.nsw.gov.au
Subject: Processors
Hello Bill,
You said your VIP was reliable . I agree, we have one
too and it is a good instrument.
It seems no one may get a chance to get a Peloris as
the competition says Vision Biosystems is FOR SALE or
looking for a distributor in the USA.
You are in Australia and the Histology field. Thie
website says they are in Mt.Waverly, VIC, Australia.
Is that near you? Have you heard any word on their
Boss going door to door looking for a buyer in the USA
?
Saya Narra
UofT
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Message: 13
Date: Fri, 15 Apr 2005 07:35:31 +1000
From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
Subject: RE: [Histonet] Long and short biopsy runs with one processor
To: <dahmed <@t> mdanderson.org>, "histonet \(E-mail\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001501c54139$e27d9730$0ecd080a <@t> wsahs.nsw.gov.au>
Content-Type: text/plain; charset="US-ASCII"
We occasionally run cell blocks but most times they are put through the
routine overnight processing.
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: dahmed <@t> mdanderson.org [mailto:dahmed <@t> mdanderson.org]
Sent: Thursday, 14 April 2005 11:13 PM
To: Bill Sinai
Subject: RE: [Histonet] Long and short biopsy runs with one processor
What about cell blocks? Do you feel that your currenct processing schedule
for biopsies would be appropriate for cytology cell blocks? Our current
bx/cell block protocol is 20 minutes in all stations including formalin for
a total run time of 6 hours? Can I trim this back a little?
David S. Ahmed
Chief Histology Technician
Department of Pathology
M.D. Anderson Cancer Center
"Bill Sinai"
<bills <@t> icpmr.wsahs.nsw.gov.au> To:
Sent by: "'Roxanne Soto'"
<godsgirlnow <@t> MSN.COM>, "histonet (E-mail)"
histonet-bounces <@t> lists.utsouth
<histonet <@t> lists.utsouthwestern.edu>
western.edu cc:
04/13/2005 04:55 PM
Subject:
RE: [Histonet]
Long and short biopsy runs with one processor
Roxanne,
Most are the small GE biopsies >3mm in diamater or "thin" slices of any
urgents >3mm thick.
Solution Time
Formalin 0min
70%ethanol 5min
95% 5min
100% 5min
100% 10min
100% 10min
Xylol 5min
Xylol 5min
Xylol 10min
Wax 5min
Wax 10min
Wax 10min
Temperature ambient with Pressur/Vacuum on all stations.
Two Leica ASP300 and two VIP 3000
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: Roxanne Soto [mailto:godsgirlnow <@t> MSN.COM]
Sent: Thursday, 14 April 2005 7:37 AM
To: Bill Sinai
Subject: Re: [Histonet] Long and short biopsy runs with one processor
What size is the tissue on the 2 hour run? How long is each station?
What kind of processor? Do you use the vacuum?
Roxanne
----- Original Message -----
From: Bill Sinai
To: histonet (E-mail)
Sent: Wednesday, April 13, 2005 5:30 PM
Subject: RE: [Histonet] Long and short biopsy runs with one processor
Julia,
I agree with your philosophy about small runs. We are a pathology
department supporting a large teaching hospital and an even larger area
pathology service. However, we also do a considerable amount of
private
work for several endoscopy clinics in our locality.
The scenario you describe is very similar to ours.
We have the usual overnight run, usually 450-600 blocks with the large
material in one processor and the smaller in another. We receive the
endoscopy specimens anytime from 7:30am through to 3:00pm each day,
with
any
pick ups after 6:00pm being processed the next morning in a short run
2-2.5hrs. This means we can have at least two runs per day of
endoscopy
specimens. The endoscopy specimens from the private clinics are about
30%
of our work and the TAT for these specimens is >2 days from pick-up to
hard
copy result to the clinician.
We also process renal biopsies several times per day as well as the
occasional urgent specimen.
Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Australia
Ph 02 9845 7774
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Julia
Dahl
Sent: Thursday, 14 April 2005 4:13 AM
To: Joyce.Rush <@t> sjmcmn.org; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Long and short biopsy runs with one processor
Joyce -
First let me preface with my bias - I am a GI pathologist (read small
biopsy
material is what I live and breathe).
Long processor runs are great for standard surgical material - but
absolutely overprocess small pieces of tissue, resulting in hard,
dehydrated, difficult to cut little brittle fragments.
The best approach that I've seen and used is to examine your
"bottlenecks."
The main bottlenecks are the points at which you have lots to do in
front of
you - with limited resource to do it (i.e. you have 150 cassettes to
embed
coming off of the processor at one time and ONE embedding station.
That's a
bottleneck.)
What time do you usually start your processor? Say your processor
starts at
10:00 p.m. with a standard 6 hour process run. Your pathologists or
your
PAs close the grossing stations at 6:00 p.m. and load the processor -
and
everything sits there for 4 hours (that's another bottleneck).
In this scenario - you can add a second processor run at virtually any
time
that you are staffed that allows you to turn over the processor for the
standard run by 6:00 p.m. (when the standard next load is).
Since a small biopsy run usually takes about 2 hours on the Vitek:
0600 - Standard processor emptied - large surgical cases ONLY (about
2/3
of
your blocks)
0600 - 1000: Embed, cut, stain and coverslip surgicals
0700 - 0800: Previous day's and early AM courier run of small biopsies
grossed in
0800 - 1000: Small biopsy processor run
1000 - small biopsy processor emptied
1000 - 1230: Embed, cut, stain and coverslip biopsies.
If you wanted to do another biopsy run - to allow same day turnaround
time -
the 2nd biopsy run could be scheduled at 10:30 each day with slides
coming
out around 3:30.
I'm interested to hear other people's ideas.
Julia
>From: "Rush, Joyce" <Joyce.Rush <@t> sjmcmn.org>
>To: <Histonet <@t> lists.utsouthwestern.edu>
>Subject: [Histonet] Long and short biopsy runs with one processor
>Date: Wed, 13 Apr 2005 12:57:07 -0500
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>
>We are a community hospital and last year processed 28,000 blocks. We
are
>being challenged by our Laboratory Director to run a long and short,
biopsy
>run daily, M-F. Currently we run everything together. We have one
>processor, a VIP5. I'd love to hear of ways people have handled this.
>Thanks so much! I always get such good advice from this group!
>
>Joyce
>
>Joyce A. Rush, BS, MT(ASCP)
>Laboratory Manager
>St Joseph's Medical Center
>523 North Third Street
>Brainerd, MN 56401
>Office:218-828-7500 Fax: 218-828-7510
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Any views expressed in this email are those of the individual sender
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Any views expressed in this email are those of the individual sender
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------------------------------
Message: 14
Date: Thu, 14 Apr 2005 18:20:11 -0700 (PDT)
From: saya narra <sayanarra <@t> yahoo.com>
Subject: [Histonet] Distributor
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050415012011.27372.qmail <@t> web60617.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Rick Couture,
Thank you for the information. I will speak with our
Lab Manager and have her contact the Distributor of
the Vision Biosystem Instruments directly, should we
decide on the need to purchase. Who was the
Distributor you spoke of again ?
saya narra
UofT
__________________________________
Do you Yahoo!?
Yahoo! Small Business - Try our new resources site!
http://smallbusiness.yahoo.com/resources/
------------------------------
Message: 15
Date: Fri, 15 Apr 2005 00:48:07 EDT
From: JMyers1 <@t> aol.com
Subject: [Histonet] Re: Histonet Posting - Tampa Area
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1ab.36836df6.2f90a187 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Carol:
I am quite familiar with this institution; I worked as the lab manager in
another hospital that's just a few miles away, and I've known several of the
techs who have worked here. Please feel free to call/write me...
Joe Myers, M.S., CT(ASCP)
Clearwater, FL
727-504-0250
********************************
Message: 8
Date: Wed, 13 Apr 2005 14:09:01 -0500
From: cjohnston <@t> mdanderson.org
Subject: [Histonet] Tampa Area
To: histonet <@t> pathology.swmed.edu
I am looking for anyone familiar with Sun Coast Hospital in the Tampa
area. If you could email me privately, I would appreciate it .
Carol M. Johnston HT(ASCP)
Division of Surgery
M.D. Anderson Cancer Center
1515 Holcombe Blvd. Unit 017
Houston, Texas 77030
713-745-4625
fax: 713-745-2548
------------------------------
Message: 16
Date: Fri, 15 Apr 2005 09:54:26 +0200
From: "Schmitz, Nicole" <schmitzn <@t> uni-muenster.de>
Subject: [Histonet] RNA in situ hybridization-staining problems
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<320709412D172C48A73DA1D2D0BE20820184AFD1 <@t> UKMSRV032.ukmuenster.de>
Content-Type: text/plain; charset="iso-8859-1"
In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution..
And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol?
Thank you for your help
Sincerely,
Nicole Schmitz
------------------------------
Message: 17
Date: Fri, 15 Apr 2005 03:42:50 -0700 (PDT)
From: - - <emerald_lake77 <@t> yahoo.com>
Subject: Re: [Histonet] RNA in situ hybridization-staining problems
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050415104250.78402.qmail <@t> web42302.mail.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Nicole,
I encountered the same problem and switched to the HRP-conjugated anti-DIG antibody also by Roche. I used a TSA amplification system (Biogenex - mainly used for their machines, but I was able to use it by hand also) and finally, detected with DAB. All my samples come out great! There was need for some adjustment as I was getting background initially. But I got rid of that with time adjustment and blocking steps. I hope this helps.
"Schmitz, Nicole" <schmitzn <@t> uni-muenster.de> wrote:
In our lab we perform RNA in situ hybridizations on paraffin sections. We use the Roche DIG Labelling Kit and the Nucleic Detection Kit (AP-conjugated antibody and NBT/BCIP staining). We tried to optimize the protocol but there are still some problems occuring. After getting rid of the background staining, which took quite a while we now have the problem that after the stopping of the final staining reaction the sections go on turning darker and darker over the next few days. Even the sense probe turns dark and if there is a high mRNA expression the sections sometimes turn so dark that you can see nothing anymore except the dark colour. Has anyone encountered that kind of problem before and has any suggestions? The problem is that we have to store the slides so just taking pictures right after staining is not the best solution..
And I have another question. While trying to solve the problem I found out that some people use an Poly d(T) oligonucleotide to measure RNA degradation in the tissue. Does anyone know more about that e.g. where to order and how to use? Would I have to use another protocol for that than my normal in situ protocol?
Thank you for your help
Sincerely,
Nicole Schmitz
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 18
Date: Fri, 15 Apr 2005 09:01:07 -0300
From: "Sharon Chase" <chash <@t> reg2.health.nb.ca>
Subject: [Histonet] RCC Antibody Help
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s25f82dc.045 <@t> SJRH-AS-N03.NBHEALTH>
Content-Type: text/plain; charset=US-ASCII
I am trying to establish a protocol for Vector's renal cell carcinoma
antibody. The data sheet states trypsin digestion for 30 minutes. I
obtain negative results with trypsin digestion using normal kidney and
clear cell carcinoma as controls.
I then did HIER retrieval using Biocare's reveal and borg solutions in
their decloaker. The normal kidney's proximal tubules stained but the
clear cell was patchy. Repeating the run on the following day, using the
same reagents and working dilution, I obtained weak results with normal
kidney and negative tumor cells.
A fresh daily working dilution(1/50), I can obtain constant normal
kidney staining but usually negative tumor cells.
I tried another tumor control block and leaving the antibody overnight.
Normal kidney is fine.
Open to any suggestions.
Sharon Chase
Atlantic Health Science Center
Saint John NB
Canada
------------------------------
Message: 19
Date: Fri, 15 Apr 2005 07:28:27 -0500
From: "Dawson, Glen" <GDawson <@t> dynacaremilwaukee.com>
Subject: RE: [Histonet] RCC Antibody Help
To: Sharon Chase <chash <@t> reg2.health.nb.ca>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <D2401DE71F59D71184BC00D0B7479B91D500D3 <@t> MILW_MAIL1>
Content-Type: text/plain; charset="iso-8859-1"
Sharon,
I've recently tried the RCC from Biocare and it works very well. It comes
as a pre-dilute so just follow the accompanying instructions and the work-up
is extremely easy. My only detraction is that it is an abnormally expen$ive
antibody.
Good Luck,
Glen Dawson BS, HT & QIHC (ASCP)
IHC Manager
Milwaukee, WI
-----Original Message-----
From: Sharon Chase [mailto:chash <@t> reg2.health.nb.ca]
Sent: Friday, April 15, 2005 6:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RCC Antibody Help
I am trying to establish a protocol for Vector's renal cell carcinoma
antibody. The data sheet states trypsin digestion for 30 minutes. I
obtain negative results with trypsin digestion using normal kidney and
clear cell carcinoma as controls.
I then did HIER retrieval using Biocare's reveal and borg solutions in
their decloaker. The normal kidney's proximal tubules stained but the
clear cell was patchy. Repeating the run on the following day, using the
same reagents and working dilution, I obtained weak results with normal
kidney and negative tumor cells.
A fresh daily working dilution(1/50), I can obtain constant normal
kidney staining but usually negative tumor cells.
I tried another tumor control block and leaving the antibody overnight.
Normal kidney is fine.
Open to any suggestions.
Sharon Chase
Atlantic Health Science Center
Saint John NB
Canada
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Fri, 15 Apr 2005 07:30:15 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Temp/Humidity Records?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F16987B849BBD649AA3CD478B07F81F71051B2 <@t> nmdamail.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
During a recent inspection, the SOP and documentation for room
temperature/humidity in the histo lab were noted as being absent.
Although I document instrument temperatures, I have not kept records on
room temp/humidity. It was noted that these factors may affect IHC
staining quality. If I am to begin documenting these parameters, I'd
like to know if there are any guidelines in a general sense (or a
specific one) relating to the variances and requirements. Any help
would be appreciated!
Sara A. Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
POBox 4700
Albuquerque, NM 87196
(505)841-2576
(505)841-2518 FAX
------------------------------
Message: 21
Date: Fri, 15 Apr 2005 14:38:50 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Temp/Humidity Records?
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F05F546C0 <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
So you keep accurate records.
What can you do with this information that is actually useful?
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu]
Sent: 15 April 2005 14:30
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Temp/Humidity Records?
During a recent inspection, the SOP and documentation for room
temperature/humidity in the histo lab were noted as being absent.
Although I document instrument temperatures, I have not kept records on
room temp/humidity. It was noted that these factors may affect IHC
staining quality. If I am to begin documenting these parameters, I'd
like to know if there are any guidelines in a general sense (or a
specific one) relating to the variances and requirements. Any help
would be appreciated!
Sara A. Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
POBox 4700
Albuquerque, NM 87196
(505)841-2576
(505)841-2518 FAX
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Fri, 15 Apr 2005 09:46:10 -0400
From: "Pamela A. Marcum" <pmarcum <@t> vet.upenn.edu>
Subject: RE: [Histonet] Temp/Humidity Records?
To: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Cc: histonet <@t> lists.utsouthwestern.edu, "Breeden, Sara"
<sbreeden <@t> nmdamail.nmsu.edu>
Message-ID: <1113572770.425fc5a21ff35 <@t> imp.vet.upenn.edu>
Content-Type: text/plain; charset=ISO-8859-1
The comment for the records were regarding IHC and yet I find changes in
humidity and temperature have more effect on my sectioning and mounting of
paraffin and plastic sections. If you are doing automated IHC I don't see how
this would matter. If it is manual and you use a humidity chamber it still
won't matter much. I could only see this mattering to IHC if it is done on the
bench in open air. The changes I get seasons change throughout the day as heat
or AC are added or changed.
Is this really a new requirement??
Pam Marcum
Quoting "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>:
> So you keep accurate records.
> What can you do with this information that is actually useful?
>
> Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
> Consultant Pathologist
> Rotherham General Hospital
> South Yorkshire
> England
> terry.marshall <@t> rothgen.nhs.uk
>
> -----Original Message-----
> From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu]
> Sent: 15 April 2005 14:30
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Temp/Humidity Records?
>
>
> During a recent inspection, the SOP and documentation for room
> temperature/humidity in the histo lab were noted as being absent.
> Although I document instrument temperatures, I have not kept records on
> room temp/humidity. It was noted that these factors may affect IHC
> staining quality. If I am to begin documenting these parameters, I'd
> like to know if there are any guidelines in a general sense (or a
> specific one) relating to the variances and requirements. Any help
> would be appreciated!
>
> Sara A. Breeden, HT(ASCP)
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
> POBox 4700
> Albuquerque, NM 87196
> (505)841-2576
> (505)841-2518 FAX
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 23
Date: Fri, 15 Apr 2005 09:45:13 -0500
From: "Charles Scouten" <cwscouten <@t> myneurolab.com>
Subject: RE: [Histonet] Long and short biopsy runs with one processor
To: "Rush, Joyce" <Joyce.Rush <@t> sjmcmn.org>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5784D843593D874C93E9BADCB87342AB44F84E <@t> tpiserver03.Coretech-holdings.com>
Content-Type: text/plain; charset="iso-8859-1"
We offer a processor able to simultaneously process two runs, so you can be doing the long run while ripping through several short runs. It is a dip and dunk system, like a stainer, but has thorough vapor emission control. This style has less carryover and mixing than a single tank systems, and lets more than one run be processing at any one time.
The TPC 15 DUO Tissue Processor Product: #511101 from Vibratome, made by Medite
TPC 15 DUO Tissue Processor
http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=511101&catdesc=Histology+Equipment&CatThreeID=595&CatOneID=4&subcatdesc=Tissue+Processing+Systems&idsubcategory=180
Cordially,
Charles W. Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300
FAX 314 522 0377
cwscouten <@t> myneurolab.com
http://www.myneurolab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rush, Joyce
Sent: Wednesday, April 13, 2005 12:57 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Long and short biopsy runs with one processor
We are a community hospital and last year processed 28,000 blocks. We are being challenged by our Laboratory Director to run a long and short, biopsy run daily, M-F. Currently we run everything together. We have one processor, a VIP5. I'd love to hear of ways people have handled this. Thanks so much! I always get such good advice from this group!
Joyce
Joyce A. Rush, BS, MT(ASCP)
Laboratory Manager
St Joseph's Medical Center
523 North Third Street
Brainerd, MN 56401
Office:218-828-7500 Fax: 218-828-7510
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 24
Date: Fri, 15 Apr 2005 10:50:55 -0400
From: "jo-ann-e.bader" <jo-ann.bader <@t> mcgill.ca>
Subject: [Histonet] CD31 on Mouse
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BE854D0F.80DE%jo-ann.bader <@t> mcgill.ca>
Content-Type: text/plain; charset="US-ASCII"
Good morning everyone,
I have a couple of labs in our group who would like to do immuno with CD31
on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a
method that works. Any advise will be appreciated.
Thank you in advance
Jo-Ann Bader
--
Director's Assistant
Molecular Oncology Group
McGill University Health Centre
687 Pine Ave. W. Room H5-21
Montreal, QC,Canada, H3A-1A1
Tel: 514-843-1479
Fax; 514-843-1478
E-mail: jo-ann.bader <@t> mcgill.ca
------------------------------
Message: 25
Date: Fri, 15 Apr 2005 09:17:32 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] CD31 on Mouse
To: "'jo-ann-e.bader'" <jo-ann.bader <@t> mcgill.ca>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000201c541ce$424713f0$76d48a80 <@t> AMY>
Content-Type: text/plain; charset="US-ASCII"
Jo-Ann
We use the goat polyclonal from santa cruz. Works great on FFPE tissue.
I'll send you the protocol and image off the histonet.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
jo-ann-e.bader
Sent: Friday, April 15, 2005 7:51 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 on Mouse
Good morning everyone,
I have a couple of labs in our group who would like to do immuno with
CD31
on formalin fixed, paraffin, mouse tissue. ?they cannot seem to find a
method that works. Any advise will be appreciated.
Thank you in advance
Jo-Ann Bader
--
Director's Assistant
Molecular Oncology Group
McGill University Health Centre
687 Pine Ave. W. Room H5-21
Montreal, QC,Canada, H3A-1A1
Tel: 514-843-1479
Fax; 514-843-1478
E-mail: jo-ann.bader <@t> mcgill.ca
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Message: 26
Date: Fri, 15 Apr 2005 11:36:29 -0400
From: "Charles, Roger" <rcharles <@t> state.pa.us>
Subject: RE: [Histonet] Temp/Humidity Records?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57006C557D <@t> enhbgpri04.pa.lcl>
Content-Type: text/plain; charset="us-ascii"
We went thru this same ordeal a few years ago during our AAVLD
inspection.
I also had the same comments regarding the closed conditions of
automated IHC and how room temp and humidity would have little or any
affect on IHC. The inspector explained the requirement is justified
when troubleshooting any testing that has somehow been transformed over
time based on your control changes. The data would be used to rule out
the room conditions more then to blame the room conditions as the cause.
Our whole laboratory is now wired to monitor these conditions.
Roger Charles
TSE and IHC Technician
Pa Veterinary Laboratory
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pamela
A. Marcum
Sent: Friday, April 15, 2005 9:46 AM
To: Marshall Terry Dr,Consultant Histopathologist
Cc: histonet <@t> lists.utsouthwestern.edu; Breeden,Sara
Subject: RE: [Histonet] Temp/Humidity Records?
The comment for the records were regarding IHC and yet I find changes in
humidity and temperature have more effect on my sectioning and mounting
of
paraffin and plastic sections. If you are doing automated IHC I don't
see how
this would matter. If it is manual and you use a humidity chamber it
still
won't matter much. I could only see this mattering to IHC if it is done
on the
bench in open air. The changes I get seasons change throughout the day
as heat
or AC are added or changed.
Is this really a new requirement??
Pam Marcum
Quoting "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>:
> So you keep accurate records.
> What can you do with this information that is actually useful?
>
> Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
> Consultant Pathologist
> Rotherham General Hospital
> South Yorkshire
> England
> terry.marshall <@t> rothgen.nhs.uk
>
> -----Original Message-----
> From: Breeden, Sara [mailto:sbreeden <@t> nmda.nmsu.edu]
> Sent: 15 April 2005 14:30
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Temp/Humidity Records?
>
>
> During a recent inspection, the SOP and documentation for room
> temperature/humidity in the histo lab were noted as being absent.
> Although I document instrument temperatures, I have not kept records
on
> room temp/humidity. It was noted that these factors may affect IHC
> staining quality. If I am to begin documenting these parameters, I'd
> like to know if there are any guidelines in a general sense (or a
> specific one) relating to the variances and requirements. Any help
> would be appreciated!
>
> Sara A. Breeden, HT(ASCP)
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
> POBox 4700
> Albuquerque, NM 87196
> (505)841-2576
> (505)841-2518 FAX
>
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End of Histonet Digest, Vol 17, Issue 25
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