[Histonet] Mouse femurs and tibias fixation time

[Elizabeth Chlipala]

Debra

I agree with Jackie we fix for 24-48 hours and then decal in 5% formic
acid.  We section the knees in a frontal plane, so we remove most of the
muscle prior to placement in decal.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grant,
Debra
Sent: Friday, April 08, 2005 12:18 PM
To: Histonet
Subject: [Histonet] Mouse femurs and tibias fixation time

Hi all,

I am processing some mouse femurs and tibias for IHC. Any
recommendations as to amount of fixation time for Zinc formalin before
paraffin processing?

Thanks in advance!

Cheers! 

Debby Grant
Histology Specialist I  
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
 

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