[Histonet] Universal (protein) block for IHC

[Johnson, Teri]

Has anybody converted to using a universal protein block (casein) for
their immunohistochemistry protocols rather than using species specific
normal sera?  For those who have made the conversion, did you have to
make any changes to your methodology or did you just plug it in to your
protocol and run?  Did you notice any difference in the staining
intensity for known antibodies (stronger, weaker, no change)?  Are you
also using it as a secondary antibody diluent or for washes?  Finally,
are you using commercially available reagent or are you making your own?
 
Thanks so much!
 

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org