[Histonet] beta galactosidase help!

Claudia Schaller claus <@t> itsa.ucsf.edu
Wed Apr 6 16:04:51 CDT 2005

Hi everybody,

i need urgent help with beta galactosidase detection in Bouin's fixed 
testes sections (frozen and paraffin)!

Testes got first fixed in Bouin's, then embedded in paraffin and O.C.T, 
Staining with X-gal on frozens didn't work? Does someone has any idea if 
the fixative (contains formaldehyde, picric acid, glacial acetic acid) 
destroys the enzyme?
Does X-gal work on already paraffin embedded sections?
Does someone know a good polyclonal anti beta- galactosidase antibody 
which i could use for the paraffin sections?

ANY help is highly appreciated!!!


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