[Histonet] CAP negative controls

Bonnie J. McMahill bmcmahill <@t> incytepathology.com
Tue Apr 5 12:57:10 CDT 2005


We use a multiple tissue control block for all of our immunos.  The tissue in it serves the purpose of positive and negative tissue control.  We are then able to use it for multiple antibodies.  It was fashioned after the "sausage" block that Hector Battifora made many moons ago.

Bonnie McMahill
Histology Supervisor
InCyte Pathology
Spokane, WA

> -----Original Message-----
> From:	Snyder, Wendy [SMTP:SnyderW <@t> uhcwv.org]
> Sent:	Tuesday, April 05, 2005 10:14 AM
> To:	Histonet <@t> lists.utsouthwestern.edu
> Subject:	[Histonet] CAP negative controls
> Hello,
>   I have just received the sept. 04 CAP checklist for AP.  There is a
> revised Immuno question pertaining to negative controls on it.  The
> checklist number is ANP.22570.  CAP now requires you to have 2 negative
> controls.  One negative REAGENT control and one negative TISSUE control.  I
> am wanting to put together a negative TISSUE block, a sausage block.  I am
> looking for a list of negative tissue's to use in this block to run on a
> number of immuno stains.  I would love to hear some feed back on this.  Can
> anyone help?
> Wendy Snyder HT(ASCP)
> United Hospital Center
> Clarksburg, WV
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