[Histonet] Perfusion Fixation of Mouse Hearts
histophilhuff <@t> yahoo.com
Wed Sep 29 15:17:44 CDT 2004
We currently perform routine mouse perfusions using paraformaldehyde as our fixative. We place a needle into the left ventricle of the heart and perfuse at 120 mmHg for 2-3 min, however we need to clamp the heart to the needle. I can forward you our detailed protocol for mouse perfusions, however you may have to adapt the procedure depending on the area of the heart you are interesting in analyzing. If a small hole at the apex of the heart is no problem then this protocol should work for you.
Christopher Jackson <christopherjackson69 <@t> hotmail.com> wrote:
I am a research assistant at the Ottawa Heart Institute. I am working
with mice models.
I am supposed to learn how to perfusion fix a mouse heart and then embed
it into paraffin wax. Does anyone have a protocol that I could use, or
information regarding this technique?
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