[Histonet] Water bath antigen retrieval

[Poteete, Jacquie A.]

I do the very same procedure Tom listed.

Jacquie Poteete MT(ASCP)QIHC
Lead Technologist, IHC Laboratory
Saint Francis Hospital, Tulsa, OK 74136
japoteete <@t> saintfrancis.com

> -----Original Message-----
> From:	Tom McNemar [SMTP:TMcNemar <@t> lmhealth.org]
> Sent:	Friday, September 24, 2004 6:36 AM
> To:	'Paula Pierce'; histonet <@t> lists.utsouthwestern.edu
> Subject:	RE: [Histonet] Water bath antigen retrieval
> 
> Wondering if this is your standard procedure for all antibodies?  I only
> steam for 20 minutes with a 20 minute cool for all retrieval.  Place them
> in
> buffer for 5 minutes then directly on the Autostainer.  They work
> beautifully.
> 
> Just curious,
> 
> Tom Mc Nemar HT(ASCP)
> Histology Supervisor
> Licking Memorial Hospital
> Newark, Ohio 43055
> 
> 
> 
> -----Original Message-----
> From: Paula Pierce [mailto:contact <@t> excaliburpathology.com] 
> Sent: Thursday, September 23, 2004 2:05 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Water bath antigen retrieval
> 
> 
> I use a Black and Decker rice steamer for antigen retrieval. About $30.
> Fill
> the bottom with distilled water. Place the plastic basket in the steamer.
> Place a container with your slides and citrate buffer in the basket so the
> lid will fit. Turn the steamer on for 1 hour, then let cool for 25
> minutes.
> Rinse in distilled water slowly to finish cooling. Proceed with your IHC
> stain.
>  
>  
> 
> 
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