[Histonet] Water bath antigen retrieval

[Tom McNemar]

Wondering if this is your standard procedure for all antibodies?  I only
steam for 20 minutes with a 20 minute cool for all retrieval.  Place them in
buffer for 5 minutes then directly on the Autostainer.  They work
beautifully.

Just curious,

Tom Mc Nemar HT(ASCP)
Histology Supervisor
Licking Memorial Hospital
Newark, Ohio 43055



-----Original Message-----
From: Paula Pierce [mailto:contact <@t> excaliburpathology.com] 
Sent: Thursday, September 23, 2004 2:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Water bath antigen retrieval


I use a Black and Decker rice steamer for antigen retrieval. About $30. Fill
the bottom with distilled water. Place the plastic basket in the steamer.
Place a container with your slides and citrate buffer in the basket so the
lid will fit. Turn the steamer on for 1 hour, then let cool for 25 minutes.
Rinse in distilled water slowly to finish cooling. Proceed with your IHC
stain.
 
 


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet