Hi everyone! If I want to do a double staining using one antibody that requires HIER and another that will stain everything if heated........what do I do? Can anyone help me? Thanks ! Inga -- Inga Hansson dept. neuroscience, div. neurobiology PO Box 587 Biomedical Centre Husargatan 3 S-751 23 Uppsala SWEDEN phone:+46-18-4714384 fax: +46-18-559017