[Histonet] Glycerin-Gelatine bubble-phenomen

[Barbara Bublava]

Hi Everybody!

I have an interesting phenomen mounting slides with Glyceringelatine 

When I mount my slides they do not have bubbles. Oil Red O stains do not develop bubbles even after some time but when I mount CAE - Chloracetate Esterase (I mount in the same way I do the ORO) the slides develop bubbles after some hours and after some days the whole section is covered with bubbles, but there are no bubbles around the section!

Why does this happen and how can I prevent this???

I use Glyceringelatine at 60°C, slides out of dest. water, excess water shaked of. I did also try to dry the slides bevore mounting but there happend to be more bubbles from the beginning and it did not change this strange effect.
Sometimes, when I am in a hurry I further hardening of the Glyceringelatine at fridge (4°C) but I can not see any positive or negative effect on bubbles doing that.

Thanks to everyone

Barbara, Vienna