[Histonet] CD31/PECAM in Rat Brain
nsdnz <@t> neuro.hfh.edu
Wed Sep 15 12:54:36 CDT 2004
Our lab is interested in looking at CD31/PECAM in rat brain tissue as a
marker for neovessels.
We don't know anyone in our facility who has used this antibody
extensively, so we are at a loss for deciding on a manufacturer. Has
anyone worked with this antibody and has a (successful) protocol and
manufacturer that they prefer?
We would prefer a mouse monoclonal for double labeling, but polyclonal
is ok, too. Does anyone know if we will need to use any exotic
fixation/retrieval techniques? We want to be able to work out a
protocol first in paraffin and may adapt to frozen sections later.
Thank you for any help you can provide, and if you need more info,
please let me know,
Henry Ford Health System
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