[Histonet] RE: Histonet Digest, Vol 10, Issue 19
GUTIERREZ, JUAN
juan.gutierrez <@t> christushealth.org
Wed Sep 15 08:46:12 CDT 2004
Your Ventana rep should be able and willing to set up your validation protocols, and help you get started. Good luck.
Juan C. Gutierrez, HT(ASCP)
Histology Laboratory Supervisor
(210)704-2533
-----Original Message-----
From: Shawn Salesky [mailto:ssalesky <@t> LowellGeneral.org]
Sent: Tuesday, September 14, 2004 12:39 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Histonet Digest, Vol 10, Issue 19
Hello All,
The Lab I work in is currently setting up automated IHC, ISH and
Special stains on Ventana systems.
First question... Is anyone using Ventana Inform HPV HR in a
clinical setting? and How did you validate?
Second question... Is there a standard number of cases to run in
validations of Immuno's?
Third question... What numbers did you use to validate your special
stains?
I'm pretty much at a loss, as I cannot find any literature on the
subjects.
Thanks in advance,
Shawn Salesky
TC LGH Histo
> ----------
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> uthwestern.edu]
> Sent: Tuesday, September 14, 2004 1:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 10, Issue 19
>
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> Today's Topics:
>
> 1. Re: Anti-parrafin floor (DDittus787 <@t> aol.com)
> 2. re embedding of small artery (carl hobbs)
> 3. Recover tissue sections on non-plus slides (Kim Byrnes)
> 4. RE: Recover tissue sections on non-plus slides (Bonnie Whitaker)
> 5. Re: Recover tissue sections on non-plus slides
> (chiggerson <@t> memhosp.com)
> 6. alizarin red fading (Julien)
> 7. fluorescent dyes/lables for neuronal activities? (Cao, Xudong)
> 8. RE: alizarin red fading (Barry R Rittman)
> 9. RE: Recover tissue sections on non-plus slides (Laurie Colbert)
> 10. cryomacrocut (Robin Turcotte)
> 11. cryomacrocut (Robin Turcotte)
> 12. cryomacrocut (Robin Turcotte)
> 13. cryomacrocut (Robin Turcotte)
> 14. cryomacrocut (Robin Turcotte)
> 15. Shandon Hypercenter Xp (Jim Staruk)
> 16. Superfrost Slides (Bill Sinai)
> 17. embedding of small artery_paraffin (Dr. Raghul)
> 18. vascular graft_sectioning difficulty (Dr. Raghul)
> 19. Test (Dimaano, Nena)
> 20. RE: Superfrost Slides (Joe Nocito)
> 21. Perferred fixative for animals
> (wasielewski.reinhard.von <@t> mh-hannover.de)
> 22. RE: Fixative for animals (Cullen, Kay)
> 23. Histo. Assistant Job Descriptions (Amy Self)
> 24. Re: Perferred fixative for animals (LaCinda Burchell)
> 25. Re: Recover tissue sections on non-plus slides (Barbara Lentz)
> 26. RE: Histo. Assistant Job Descriptions (Kari Bradshaw)
> 27. FW: [Histonet] Histo. Assistant Job Descriptions (Bonnie Whitaker)
> 28. rat embryos (Jeffus, Brandon)
> 29. RE: FW: [Histonet] Histo. Assistant Job Descriptions
> (Bonnie Whitaker)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 13 Sep 2004 13:48:40 -0400
> From: DDittus787 <@t> aol.com
> Subject: [Histonet] Re: Anti-parrafin floor
> To: BBEIER <@t> kumc.edu ("Barbara Beier")
> Cc: histonet <@t> pathology.swmed.edu
> Message-ID: <7B0A3F7A.56A40CDC.0A1F969F <@t> aol.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Barb:
> the flooring came from Acme Panels-the company is in the UK and can be
> found on Google, it is anti-solvent, chemical, paraffin,etc. It comes in 3
> or 4 colors and everyone loves it.
> We had a contractor bring it in and install it.
> dana
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 13 Sep 2004 19:02:14 +0100
> From: "carl hobbs" <carl.hobbs <@t> kcl.ac.uk>
> Subject: [Histonet] re embedding of small artery
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <NMEOLOHBKJDEENMCOONFEEKKCBAA.carl.hobbs <@t> kcl.ac.uk>
> Content-Type: text/plain; charset="iso-8859-1"
>
> In my experienc, with fixed tissues it is easy to embed using agarose,
> then
> to re-orient the tissue for frozen/pwax sectioning: Dissolve 1% agarose(
> aq)
> and when about 50C place the tissue in the agarose, in a bigger mould. It
> will set quickly.....better if placed at 4C for a while. Remove the set
> agarose and cut away excess agarose so you have the tissue in the
> orientation of your choice. Then process to pwax. Or, place in OCT for a
> couple of hours on a rotator. Then embed in an OCT filled mould in the
> orientation you need and....freeze. If you are doing fresh tissue, get the
> melted, cooling agarose into a mould , place the tissue and cool quickly.
> Immediately place in OCT, swirl gently for a minute, then freeze. Play
> around with control material until you are confident of the technique and
> to
> reassure yourself that the elevated temp does not adversely afffect your
> tissue. You can also do gelatin/vibratome sections of the fixed tissue, if
> you have a vibratome.
> ---
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>
> ------------------------------
>
> Message: 3
> Date: Mon, 13 Sep 2004 11:34:29 -0700 (PDT)
> From: Kim Byrnes <k_byrnes <@t> yahoo.com>
> Subject: [Histonet] Recover tissue sections on non-plus slides
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040913183429.84652.qmail <@t> web12308.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
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> ------------------------------
>
> Message: 4
> Date: Mon, 13 Sep 2004 14:24:14 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: RE: [Histonet] Recover tissue sections on non-plus slides
> To: "'Kim Byrnes'" <k_byrnes <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000301c499c7$41758920$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
> There is a procedure to remove tissue sections from a slide, and they can
> then be mounted on + slides. The product that works best in my hands is
> Mount-Quick. It has more than one distributer, but I know that Newcomer
> Supply (800-383-7799) has this product, as well as directions on how to do
> the procedure.
>
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Byrnes
> Sent: Monday, September 13, 2004 1:34 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Recover tissue sections on non-plus slides
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
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>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 13 Sep 2004 14:46:54 -0500
> From: chiggerson <@t> memhosp.com
> Subject: Re: [Histonet] Recover tissue sections on non-plus slides
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Kim Byrnes <k_byrnes <@t> yahoo.com>
> Message-ID:
> <OFA20F95F7.6FBEE73B-ON86256F0E.006C3CA9-86256F0E.006CAA8F <@t> pmmc.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Kim,
>
> The 2000 Tech Sample exercise HT-1 gives a procedure for this. The title
> of the exercise is "Use Mount Quick to Remove Tissue Sections from
> Non-Positively Charged Slides for Immunohistochemical and Special Stains".
>
>
> Good luck!
>
> Cindy
>
> Cindy Higgerson HTL(ASCP)
> Surgical Pathology Supervisor
> Memorial Hospital
>
>
>
>
>
> Kim Byrnes <k_byrnes <@t> yahoo.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 09/13/2004 01:34 PM
>
> To
> histonet <@t> lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Recover tissue sections on non-plus slides
>
>
>
>
>
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 13 Sep 2004 16:58:59 -0400
> From: "Julien" <julien_lambreydesouza <@t> uqar.qc.ca>
> Subject: [Histonet] alizarin red fading
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <00c501c499d4$7dc51140$9310d784 <@t> uqar.qc.ca>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello all,
>
> We do differencial staining of bone and cartillage on small fish (~1cm).
> To do so, we use the Clear and Stain technique which results in red bone
> coloration and blue cartillage coloration. After a month or so, We noticed
> that the red stain tends to fade off. We tried to re-stain the fish, but
> the alizarin red wouldn't stain the bones anymore.
>
> Why is this so? Is it that bone decalcifies in the glycerin? We put a few
> cristals of thymol in the glycerin to avoid mold development. Could this
> be a decalcifying agent? Does anybody know of a way to re-stain?
>
> For the time being, we score bony elements within 3 days of staining to
> avoid fading problems, but this stops us from doing batch staining. Batch
> staining would be much faster. We would then keep stained fish in glycerin
> until scoring.
>
> Thanks for any suggestion.
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
> (418) 723-1986 #1438
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 13 Sep 2004 17:03:50 -0400
> From: "Cao, Xudong" <Xudong_Cao <@t> brown.edu>
> Subject: [Histonet] fluorescent dyes/lables for neuronal activities?
> To: <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1DA88DDC48CCC245A777599FDAEED6D0A3909B <@t> MAIL1.AD.Brown.Edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Greetings! first of all I'd like to thank eveybody who responded to my
> previous posts. The advice/ideas I received were valuable! Here I have
> one more question: I am studying nerve innervation into artificial skin
> graft that we make in vitro and my immunostaining tells me that there are
> some wonderful nerve fibers growing into the skin. The question that we
> have now is if the innervation functional. In order to test this, we want
> to mechanically tease the artificial skin and see if there is some
> responses from the nerve cells that have presumablly innervated the
> corresponding part of the skin. Thus, it would be nice to find some
> fluorescent dyes/lables that will glow when the neurons are
> activated/firing. any suggestions?
>
> best regards,
>
> Xudong
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 13 Sep 2004 16:29:32 -0500
> From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
> Subject: RE: [Histonet] alizarin red fading
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <566FB0B522443D43AF02D2ADBE35A6F0F13357 <@t> UTHEVS3.mail.uthouston.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I cannot see why the addition of a few crystals of thymol would make the
> glycerin acidic enough to cause removal of calcium and thus leaching of
> the alizarin.
> If you are using 100% glycerin, there should be no need for the addition
> of thymol.
> I am not sure exactly how you have processed these specimens. If you are
> using the classical method with potassium hydroxide (as the leaching agent
> to remove excess stain from soft tissues), is it possible that there were
> traces of potassium hydroxide left in the specimen?
> I have some specimens that were stained in 1961 and some in 1970 that show
> no signs of fading.
> Barry
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Julien
> Sent: Monday, September 13, 2004 3:59 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] alizarin red fading
>
> Hello all,
>
> We do differencial staining of bone and cartillage on small fish (~1cm).
> To do so, we use the Clear and Stain technique which results in red bone
> coloration and blue cartillage coloration. After a month or so, We noticed
> that the red stain tends to fade off. We tried to re-stain the fish, but
> the alizarin red wouldn't stain the bones anymore.
>
> Why is this so? Is it that bone decalcifies in the glycerin? We put a few
> cristals of thymol in the glycerin to avoid mold development. Could this
> be a decalcifying agent? Does anybody know of a way to re-stain?
>
> For the time being, we score bony elements within 3 days of staining to
> avoid fading problems, but this stops us from doing batch staining. Batch
> staining would be much faster. We would then keep stained fish in glycerin
> until scoring.
>
> Thanks for any suggestion.
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
> (418) 723-1986 #1438
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 13 Sep 2004 14:43:49 -0700
> From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
> Subject: RE: [Histonet] Recover tissue sections on non-plus slides
> To: "Kim Byrnes" <k_byrnes <@t> yahoo.com>, "Histonet (E-mail)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <0BE6ADFAE4E7E04496BF21ABD346628001C5C062 <@t> EXCHANGE1.huntingtonhospital.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We have used the Mount Quick from Newcomer Supply, and it works well.
>
> Laurie Colbert
> Huntington Memorial Hospital
> Pasadena, CA
>
> -----Original Message-----
> From: Kim Byrnes [mailto:k_byrnes <@t> yahoo.com]
> Sent: Monday, September 13, 2004 11:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Recover tissue sections on non-plus slides
>
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
> http://promotions.yahoo.com/new_mail
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 13 Sep 2004 20:01:58 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000701c499ee$cb688f70$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 13 Sep 2004 20:07:19 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000e01c499ee$ee9cdbe0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 13 Sep 2004 20:07:47 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000f01c499ee$ef064c10$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 13 Sep 2004 20:10:32 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <001301c499ef$45100dd0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 13 Sep 2004 20:11:36 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <001c01c499ef$6fce96e0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
> ------------------------------
>
> Message: 15
> Date: Mon, 13 Sep 2004 21:15:52 -0400
> From: "Jim Staruk" <jstaruk <@t> masshistology.com>
> Subject: [Histonet] Shandon Hypercenter Xp
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000201c499f8$87d3cb80$6401a8c0 <@t> yourw04gtxld67>
> Content-Type: text/plain; charset="us-ascii"
>
> I'm looking for someone familiar with the Shandon Hypercenter Xp tissue
> processor. I need some technical advise. Can someone help me? Anyone
> planning to invoice me for their help need not reply.
>
> Thank you
>
> Jim
>
> ________________________
> James E. Staruk, HT(ASCP)
> Mass Histopathology Service
> www.masshistology.com
>
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 14 Sep 2004 13:43:17 +1000
> From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
> Subject: [Histonet] Superfrost Slides
> To: "histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000101c49a0c$f9356e50$e1ce080a <@t> wsahs.nsw.gov.au>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Is anyone having troubles with NO staining on some slides on the Ventana
> Benchmark.
> The biggest problem seems to be with the EDTA buffered CC1 retrieval
> solution.
> Not all slides are effected, just an occasional one, but we usually end up
> with little or no staining with anything including the Haematoxylin.
> We use Menzel Superforst Plus and Superfrost Plus Ultra, and both slides
> have shown the effect.
>
> Thanks
> Bill Sinai
> Laboratory Manager
> Tissue Pathology, ICPMR
> Westmead NSW 2145
> Australia
> Ph 02 9845 7774
>
>
> __________________________________________________________________
>
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> ------------------------------
>
> Message: 17
> Date: Tue, 14 Sep 2004 16:31:15 +0530
> From: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>
> Subject: [Histonet] embedding of small artery_paraffin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <WorldClient-F200409141631.AA31150971 <@t> sctimst.ker.nic.in>
>
> hello galina,
>
> Rat and mouse organs can be cut easily after short processing(using
> acetone and xylene)and then embedding in paraffin wax of low melting point
> (56 C). We have followed the protocols from below given reference
> successfully and same could applied for the small artery also
>
> Theory and practice of Histological techniques (bancroft and steven)
>
> (or)
>
> Manual of histologic staining methods of the armed forces institutes of
> pathology edited by Lee G. Luna, III edition page 16
>
>
> Raghul J
> Histopathology-Implant Biology
> Sree chitra tirunal institute
> Thiruvananthapuram 695 012
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 14 Sep 2004 16:56:23 +0530
> From: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>
> Subject: [Histonet] vascular graft_sectioning difficulty
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <WorldClient-F200409141656.AA56230975 <@t> sctimst.ker.nic.in>
> Content-Type: text/plain; charset="iso-8859-1"
>
> hello members,
>
> We cut 5 µm thin sections of paraffin embedded vascular graft(Dacron) in
> a rotary microtome with disposable blades but the senior tech tells that
> the tissue sections are crumbling and forces us to go for thick sections
> around 10µm but again not with much success.
>
> We can understand that there is not enough tissue adhereing to the
> graft and we are literally cutting the graft. But did anyone had such
> problems sectioning vascular grafts. Any suggestions?
>
>
> regards ,
>
> Raghul J
> scientist
> Histopathology- implant biology division
> Srichitra tirunal institute of medical sciences and technology
> Trivandrum -695 012
> Kerala, India
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 14 Sep 2004 07:54:55 -0400
> From: "Dimaano, Nena" <nena.dimaano <@t> stryker.com>
> Subject: [Histonet] Test
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD06210EE4 <@t> HOS2KEXCHCL.howost.strykercorp.c
> om>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Nena Dimaano
> Advanced Technology
> Stryker Orthopaedics
> 325 Corporate Drive
> Mahwah, NJ 07430
> tel: 201-831-5338
> fax: 201-831-6224
> email: Nena.Dimaano <@t> stryker.com
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 14 Sep 2004 07:09:09 -0500
> From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
> Subject: RE: [Histonet] Superfrost Slides
> To: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>, "histonet
> \(E-mail\)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <JFEMICGBHEGPLAMIJPJPAEHICGAA.JNocito <@t> Pathreflab.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Bill,
> we've had problems also. It could be that the slides might be too changed,
> which is causing the reagents to bead up. Currently, we are using positive
> charged slides from Statlab Medical Products, which I think they get from
> Erie Scientific. Statlab's number is 1-800-442-3573. The may be able to
> direct you to some one closer to you.
>
> Joe Nocito, BS, HT(ASCP) QIHC
> Histology Manager
> Pathology Reference Lab
> San Antonio, TX
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bill
> Sinai
> Sent: Monday, September 13, 2004 10:43 PM
> To: histonet (E-mail)
> Subject: [Histonet] Superfrost Slides
>
>
>
> Is anyone having troubles with NO staining on some slides on the Ventana
> Benchmark.
> The biggest problem seems to be with the EDTA buffered CC1 retrieval
> solution.
> Not all slides are effected, just an occasional one, but we usually end up
> with little or no staining with anything including the Haematoxylin.
> We use Menzel Superforst Plus and Superfrost Plus Ultra, and both slides
> have shown the effect.
>
> Thanks
> Bill Sinai
> Laboratory Manager
> Tissue Pathology, ICPMR
> Westmead NSW 2145
> Australia
> Ph 02 9845 7774
>
>
> __________________________________________________________________
>
> This electronic message and any attachments may be confidential. If you
> are not the intended recipient of this message would you please delete the
> message and any attachments and advise the sender. Western Sydney
> Area Health Services (WSAHS) uses virus scanning software but excludes
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>
> This email may contain privileged and confidential information intended
> only for the use of the addressees named above. If you are not the
> intended recipient of this email, you are hereby notified that any use,
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 14 Sep 2004 14:29:10 +0200
> From: wasielewski.reinhard.von <@t> mh-hannover.de
> Subject: [Histonet] Perferred fixative for animals
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <41470036.30954.8F79EDC3 <@t> localhost>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Histonetters,
> We are looking for the most convenient and morpholgical satisfying
> fixative for
> mouse and rat tissue beside formaldehyde. We want to stain a wide range of
>
> different immunomarkers but don' t like formalin (therefore) too much.
> Frozen
> sections are too bad in morphology.
> Please send me any suggestions !
>
> Many thanks in advance,
> Reinhard.
>
>
>
> PD Dr. med. Reinhard von Wasielewski
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 14 Sep 2004 08:56:22 -0400
> From: "Cullen, Kay" <Kay.Cullen <@t> umassmed.edu>
> Subject: [Histonet] RE: Fixative for animals
> To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <B735CCD4E292B440807A7AA8CB61C6CF391DA2 <@t> edunivmail03.ad.umassmed.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Are you busing straight formaldehyde or formaldehyde/glutaraldehyde? I
> get very good results with Bouin's for most tissue.
>
> Kay Cullen
> Research Associate
> Department of Cell Biology
> University of Massachusetts Medical School
> Worcester, MA USA
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 14 Sep 2004 10:05:39 -0400
> From: "Amy Self" <ASelf <@t> gmhsc.com>
> Subject: [Histonet] Histo. Assistant Job Descriptions
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <39836CD6DB61654E8F95A35898C921860A87F3 <@t> exchange.gmhpost.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering if anyone had any job descriptions as well as job requirements
> that they could share with me? We have one but it very minimal as to what
> the histo assistant should and should not do so our lab manager would like
> to see what other hospitals are doing and requiring for this job position.
> Thanks in advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify us immediately by replying to this message and deleting it from
> your computer. Thank you.
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 14 Sep 2004 09:16:54 -0500
> From: "LaCinda Burchell" <ljb <@t> medicine.wisc.edu>
> Subject: Re: [Histonet] Perferred fixative for animals
> To: <histonet <@t> lists.utsouthwestern.edu>,
> <wasielewski.reinhard.von <@t> mh-hannover.de>
> Message-ID: <s146b728.080 <@t> gwia.medicine.wisc.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Dear Reinhard,
> We use Prefer fixative from Anatech Ltd, www.anatechltdusa. We have
> beautiful morphology, staining is wonderful (specials and immunos). The
> only down side of Prefer is that it affects the staining of eosinophils.
> We've recently learned how to get around that issue in human tissues,
> but haven't figured it out in rat tissues. Best of luck! Cindy B
>
> LaCinda Burchell, BA, AS, HT(ASCP)
> University of Wisconsin-Madison, Medical School
> Asthma and Allergy Research IHC Lab
> 600 Highland Ave. CSC K4/913
> Madison, Wisconsin 53792
>
> Phone: 608-262-3518
> FAX: 608-263-3746
>
> >>> <wasielewski.reinhard.von <@t> mh-hannover.de> 09/14/04 07:29AM >>>
> Hi Histonetters,
> We are looking for the most convenient and morpholgical satisfying
> fixative for
> mouse and rat tissue beside formaldehyde. We want to stain a wide range
> of
> different immunomarkers but don' t like formalin (therefore) too much.
> Frozen
> sections are too bad in morphology.
> Please send me any suggestions !
>
> Many thanks in advance,
> Reinhard.
>
>
>
> PD Dr. med. Reinhard von Wasielewski
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 14 Sep 2004 10:48:20 -0400
> From: "Barbara Lentz" <Barbara_Lentz <@t> dahlchase.com>
> Subject: Re: [Histonet] Recover tissue sections on non-plus slides
> To: <histonet <@t> lists.utsouthwestern.edu>, <k_byrnes <@t> yahoo.com>
> Message-ID: <s146cc7a.059 <@t> dahlchase.com>
> Content-Type: text/plain; charset=US-ASCII
>
> We, too, use the Mount Quick from Newcomer Supply. On a regular basis,
> we remove H&E tissue sections to perform immunos.
> www.newcomersupply.com is their address, Barb
>
> >>> Kim Byrnes <k_byrnes <@t> yahoo.com> 09/13/04 02:34PM >>>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
> http://promotions.yahoo.com/new_mail
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 26
> Date: Tue, 14 Sep 2004 07:41:17 -0700
> From: "Kari Bradshaw" <kbradshaw <@t> lcpath.com>
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
> To: "Amy Self" <ASelf <@t> gmhsc.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <JMELLDIIEJFPHMONOAAFIEIACHAA.kbradshaw <@t> lcpath.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Amy Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 27
> Date: Tue, 14 Sep 2004 10:54:03 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: FW: [Histonet] Histo. Assistant Job Descriptions
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000801c49a73$0ee10140$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Here is ours.
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kari
> Bradshaw
> Sent: Tuesday, September 14, 2004 9:41 AM
> To: Amy Self; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Amy Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 28
> Date: Tue, 14 Sep 2004 11:33:48 -0500
> From: "Jeffus, Brandon" <JeffusBrandon <@t> uams.edu>
> Subject: [Histonet] rat embryos
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <748D4F173E342D47B69FC23C87E5913C0125E7D4 <@t> EXCHANGE4.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello all... im rather new to immunohistochemistry so bear with me. I'm
> attempting to process some rat embryos for staining with a primary Ab
> and then a Cy3 labeled secondary for visualization of protein
> localization. I'm wondering if the best processing method would be
> frozen sections of the samples or paraffin embedding the samples? Any
> information would be greatly appreciated.
>
>
>
> Brandon C Jeffus
>
> Graduate Student
>
>
>
> ------------------------------
>
> Message: 29
> Date: Tue, 14 Sep 2004 11:40:24 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: RE: FW: [Histonet] Histo. Assistant Job Descriptions
> To: "'Nita Searcy'" <NSEARCY <@t> swmail.sw.org>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000001c49a79$88e006c0$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
>
> OOPS... attachments bad!! Pasting good.
>
>
> Below is our aid job description.
>
> Bonnie Whitaker
>
>
> Job Description for Laboratory/Grossing Aid
>
>
>
>
> Reports To: Laboratory Manager
>
> Pathologists
>
>
>
> Qualifications:
>
>
>
> Education: high school student or have
> diploma
> or GED
>
>
>
> Experience: not required
>
>
>
> Certification: not required
>
>
>
> Personal: The Lab/Grossing Aid must
> demonstrate an ability to listen carefully and follow directions well.
> The
> incumbent must quickly grasp the work-flow of specimen accessioning and
> grossing and become familiar with these procedures. The incumbent must
> demonstrate reasonable intelligence, diligence, accuracy, cooperation,
> neatness, and promptness in keeping with the prestige and image of this
> laboratory. The incumbent takes guidance and instruction from the
> pathologists, with whom they are working, as well as the Laboratory
> Manager
> and Executive Director.
>
> Responsibilities: The Lab/Grossing Aid will be
> responsible for the following duties:
>
> 1. Accessioning specimens and assisting with grossing procedures
> including: cassette labeling, pulling specimens for additional tissue
> submission, and discarding specimens in accordance with policy/procedure
> manual.
>
> 2. Assisting with the performance of routine maintenance and QC
> procedures on equipment to include but not limited to cryostat, grossing
> area, stain setup and filters.
>
> 3. Maintenance of inventory
>
> 4. Routine changing of stains with appropriate records maintenance.
>
> 5. Cleaning of the grossing area and instruments.
>
> 6. Any filing or routine clerical duties necessary.
>
> 7. Other duties as assigned.
>
>
>
>
> General:
>
> 1. The Lab/Grossing Aid shall be responsible for maintaining all
> company
> records of a proprietary or confidential nature secure and confidential
> and
> shall return all lists of clients, potential clients, and all other
> records,
> at Brown & Associates request.
>
> 2. The Lab/Grossing Aid is responsible for representing B&AML in a
> professional and dignified manner befitting the status of the laboratory
> in
> the Houston Medical Community.
>
> 3. The Lab/Grossing Aid will be expected to comply with all policies
> of
> B&AML as stated in the employee handbook and any newly instituted policies
> not specifically stated in the handbook. Will be expected to keep
> assigned
> work area as neat and clean as possible so as to afford the maximum
> functional usage of the assigned area. Will be expected to keep all
> records
> and files available and accessible to authorized personnel at all times.
>
> 4. Lab/Grossing Aid must be in reasonable good health with no record
> of
> chronic illness resulting in undue absence from work.
>
>
>
> I, the undersigned, have carefully read the above job description and
> fully
> understand all that is stated herein. I agree to perform all of the
> duties
> above as well as any other duties deemed necessary by my supervisor within
> the limits described here.
>
>
>
>
>
> _________________________________________
> _____________________
>
>
> EMPLOYEE SIGNATURE
> DATE
>
>
>
>
>
>
>
> _________________________________________
> _____________________
>
> LABORATORY MANAGER DATE
>
>
>
>
>
>
>
>
>
> Bonnie Whitaker
>
> -----Original Message-----
> From: Nita Searcy [mailto:NSEARCY <@t> swmail.sw.org]
> Sent: Tuesday, September 14, 2004 11:30 AM
> To: bwhitaker <@t> brownpathology.com
> Subject: Re: FW: [Histonet] Histo. Assistant Job Descriptions
>
>
>
> Bonnie, I can't view your description- could you fax? 254-724-4391
>
> Thanks!
>
> >>> "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com> 9/14/2004 10:54:03 AM
> >>>
>
> Here is ours.
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> <mailto:histonet-bounces <@t> lists.utsouthwestern.edu%5d> On Behalf Of Kari
> Bradshaw
> Sent: Tuesday, September 14, 2004 9:41 AM
> To: Amy Self; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On
> <mailto:histonet-bounces <@t> lists.utsouthwestern.edu%5dOn> Behalf Of Amy
> Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 10, Issue 19
> ****************************************
>
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