[Histonet] RE: Histonet Digest, Vol 10, Issue 19
Shawn Salesky
ssalesky <@t> LowellGeneral.org
Tue Sep 14 12:38:59 CDT 2004
Hello All,
The Lab I work in is currently setting up automated IHC, ISH and
Special stains on Ventana systems.
First question... Is anyone using Ventana Inform HPV HR in a
clinical setting? and How did you validate?
Second question... Is there a standard number of cases to run in
validations of Immuno's?
Third question... What numbers did you use to validate your special
stains?
I'm pretty much at a loss, as I cannot find any literature on the
subjects.
Thanks in advance,
Shawn Salesky
TC LGH Histo
> ----------
> From:
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> uthwestern.edu] on behalf of
> histonet-request <@t> lists.utsouthwestern.edu[SMTP:histonet-request <@t> lists.utso
> uthwestern.edu]
> Sent: Tuesday, September 14, 2004 1:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 10, Issue 19
>
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> Today's Topics:
>
> 1. Re: Anti-parrafin floor (DDittus787 <@t> aol.com)
> 2. re embedding of small artery (carl hobbs)
> 3. Recover tissue sections on non-plus slides (Kim Byrnes)
> 4. RE: Recover tissue sections on non-plus slides (Bonnie Whitaker)
> 5. Re: Recover tissue sections on non-plus slides
> (chiggerson <@t> memhosp.com)
> 6. alizarin red fading (Julien)
> 7. fluorescent dyes/lables for neuronal activities? (Cao, Xudong)
> 8. RE: alizarin red fading (Barry R Rittman)
> 9. RE: Recover tissue sections on non-plus slides (Laurie Colbert)
> 10. cryomacrocut (Robin Turcotte)
> 11. cryomacrocut (Robin Turcotte)
> 12. cryomacrocut (Robin Turcotte)
> 13. cryomacrocut (Robin Turcotte)
> 14. cryomacrocut (Robin Turcotte)
> 15. Shandon Hypercenter Xp (Jim Staruk)
> 16. Superfrost Slides (Bill Sinai)
> 17. embedding of small artery_paraffin (Dr. Raghul)
> 18. vascular graft_sectioning difficulty (Dr. Raghul)
> 19. Test (Dimaano, Nena)
> 20. RE: Superfrost Slides (Joe Nocito)
> 21. Perferred fixative for animals
> (wasielewski.reinhard.von <@t> mh-hannover.de)
> 22. RE: Fixative for animals (Cullen, Kay)
> 23. Histo. Assistant Job Descriptions (Amy Self)
> 24. Re: Perferred fixative for animals (LaCinda Burchell)
> 25. Re: Recover tissue sections on non-plus slides (Barbara Lentz)
> 26. RE: Histo. Assistant Job Descriptions (Kari Bradshaw)
> 27. FW: [Histonet] Histo. Assistant Job Descriptions (Bonnie Whitaker)
> 28. rat embryos (Jeffus, Brandon)
> 29. RE: FW: [Histonet] Histo. Assistant Job Descriptions
> (Bonnie Whitaker)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 13 Sep 2004 13:48:40 -0400
> From: DDittus787 <@t> aol.com
> Subject: [Histonet] Re: Anti-parrafin floor
> To: BBEIER <@t> kumc.edu ("Barbara Beier")
> Cc: histonet <@t> pathology.swmed.edu
> Message-ID: <7B0A3F7A.56A40CDC.0A1F969F <@t> aol.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Barb:
> the flooring came from Acme Panels-the company is in the UK and can be
> found on Google, it is anti-solvent, chemical, paraffin,etc. It comes in 3
> or 4 colors and everyone loves it.
> We had a contractor bring it in and install it.
> dana
>
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 13 Sep 2004 19:02:14 +0100
> From: "carl hobbs" <carl.hobbs <@t> kcl.ac.uk>
> Subject: [Histonet] re embedding of small artery
> To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <NMEOLOHBKJDEENMCOONFEEKKCBAA.carl.hobbs <@t> kcl.ac.uk>
> Content-Type: text/plain; charset="iso-8859-1"
>
> In my experienc, with fixed tissues it is easy to embed using agarose,
> then
> to re-orient the tissue for frozen/pwax sectioning: Dissolve 1% agarose(
> aq)
> and when about 50C place the tissue in the agarose, in a bigger mould. It
> will set quickly.....better if placed at 4C for a while. Remove the set
> agarose and cut away excess agarose so you have the tissue in the
> orientation of your choice. Then process to pwax. Or, place in OCT for a
> couple of hours on a rotator. Then embed in an OCT filled mould in the
> orientation you need and....freeze. If you are doing fresh tissue, get the
> melted, cooling agarose into a mould , place the tissue and cool quickly.
> Immediately place in OCT, swirl gently for a minute, then freeze. Play
> around with control material until you are confident of the technique and
> to
> reassure yourself that the elevated temp does not adversely afffect your
> tissue. You can also do gelatin/vibratome sections of the fixed tissue, if
> you have a vibratome.
> ---
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>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 13 Sep 2004 11:34:29 -0700 (PDT)
> From: Kim Byrnes <k_byrnes <@t> yahoo.com>
> Subject: [Histonet] Recover tissue sections on non-plus slides
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20040913183429.84652.qmail <@t> web12308.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
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> ------------------------------
>
> Message: 4
> Date: Mon, 13 Sep 2004 14:24:14 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: RE: [Histonet] Recover tissue sections on non-plus slides
> To: "'Kim Byrnes'" <k_byrnes <@t> yahoo.com>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000301c499c7$41758920$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
> There is a procedure to remove tissue sections from a slide, and they can
> then be mounted on + slides. The product that works best in my hands is
> Mount-Quick. It has more than one distributer, but I know that Newcomer
> Supply (800-383-7799) has this product, as well as directions on how to do
> the procedure.
>
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Byrnes
> Sent: Monday, September 13, 2004 1:34 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Recover tissue sections on non-plus slides
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
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>
>
>
> ------------------------------
>
> Message: 5
> Date: Mon, 13 Sep 2004 14:46:54 -0500
> From: chiggerson <@t> memhosp.com
> Subject: Re: [Histonet] Recover tissue sections on non-plus slides
> To: histonet <@t> lists.utsouthwestern.edu
> Cc: Kim Byrnes <k_byrnes <@t> yahoo.com>
> Message-ID:
> <OFA20F95F7.6FBEE73B-ON86256F0E.006C3CA9-86256F0E.006CAA8F <@t> pmmc.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Kim,
>
> The 2000 Tech Sample exercise HT-1 gives a procedure for this. The title
> of the exercise is "Use Mount Quick to Remove Tissue Sections from
> Non-Positively Charged Slides for Immunohistochemical and Special Stains".
>
>
> Good luck!
>
> Cindy
>
> Cindy Higgerson HTL(ASCP)
> Surgical Pathology Supervisor
> Memorial Hospital
>
>
>
>
>
> Kim Byrnes <k_byrnes <@t> yahoo.com>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 09/13/2004 01:34 PM
>
> To
> histonet <@t> lists.utsouthwestern.edu
> cc
>
> Subject
> [Histonet] Recover tissue sections on non-plus slides
>
>
>
>
>
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
> http://promotions.yahoo.com/new_mail
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> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 6
> Date: Mon, 13 Sep 2004 16:58:59 -0400
> From: "Julien" <julien_lambreydesouza <@t> uqar.qc.ca>
> Subject: [Histonet] alizarin red fading
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <00c501c499d4$7dc51140$9310d784 <@t> uqar.qc.ca>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello all,
>
> We do differencial staining of bone and cartillage on small fish (~1cm).
> To do so, we use the Clear and Stain technique which results in red bone
> coloration and blue cartillage coloration. After a month or so, We noticed
> that the red stain tends to fade off. We tried to re-stain the fish, but
> the alizarin red wouldn't stain the bones anymore.
>
> Why is this so? Is it that bone decalcifies in the glycerin? We put a few
> cristals of thymol in the glycerin to avoid mold development. Could this
> be a decalcifying agent? Does anybody know of a way to re-stain?
>
> For the time being, we score bony elements within 3 days of staining to
> avoid fading problems, but this stops us from doing batch staining. Batch
> staining would be much faster. We would then keep stained fish in glycerin
> until scoring.
>
> Thanks for any suggestion.
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
> (418) 723-1986 #1438
>
>
> ------------------------------
>
> Message: 7
> Date: Mon, 13 Sep 2004 17:03:50 -0400
> From: "Cao, Xudong" <Xudong_Cao <@t> brown.edu>
> Subject: [Histonet] fluorescent dyes/lables for neuronal activities?
> To: <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <1DA88DDC48CCC245A777599FDAEED6D0A3909B <@t> MAIL1.AD.Brown.Edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Greetings! first of all I'd like to thank eveybody who responded to my
> previous posts. The advice/ideas I received were valuable! Here I have
> one more question: I am studying nerve innervation into artificial skin
> graft that we make in vitro and my immunostaining tells me that there are
> some wonderful nerve fibers growing into the skin. The question that we
> have now is if the innervation functional. In order to test this, we want
> to mechanically tease the artificial skin and see if there is some
> responses from the nerve cells that have presumablly innervated the
> corresponding part of the skin. Thus, it would be nice to find some
> fluorescent dyes/lables that will glow when the neurons are
> activated/firing. any suggestions?
>
> best regards,
>
> Xudong
>
>
>
> ------------------------------
>
> Message: 8
> Date: Mon, 13 Sep 2004 16:29:32 -0500
> From: "Barry R Rittman" <Barry.R.Rittman <@t> uth.tmc.edu>
> Subject: RE: [Histonet] alizarin red fading
> To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <566FB0B522443D43AF02D2ADBE35A6F0F13357 <@t> UTHEVS3.mail.uthouston.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I cannot see why the addition of a few crystals of thymol would make the
> glycerin acidic enough to cause removal of calcium and thus leaching of
> the alizarin.
> If you are using 100% glycerin, there should be no need for the addition
> of thymol.
> I am not sure exactly how you have processed these specimens. If you are
> using the classical method with potassium hydroxide (as the leaching agent
> to remove excess stain from soft tissues), is it possible that there were
> traces of potassium hydroxide left in the specimen?
> I have some specimens that were stained in 1961 and some in 1970 that show
> no signs of fading.
> Barry
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Julien
> Sent: Monday, September 13, 2004 3:59 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] alizarin red fading
>
> Hello all,
>
> We do differencial staining of bone and cartillage on small fish (~1cm).
> To do so, we use the Clear and Stain technique which results in red bone
> coloration and blue cartillage coloration. After a month or so, We noticed
> that the red stain tends to fade off. We tried to re-stain the fish, but
> the alizarin red wouldn't stain the bones anymore.
>
> Why is this so? Is it that bone decalcifies in the glycerin? We put a few
> cristals of thymol in the glycerin to avoid mold development. Could this
> be a decalcifying agent? Does anybody know of a way to re-stain?
>
> For the time being, we score bony elements within 3 days of staining to
> avoid fading problems, but this stops us from doing batch staining. Batch
> staining would be much faster. We would then keep stained fish in glycerin
> until scoring.
>
> Thanks for any suggestion.
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
>
> Julien Lambrey de Souza
> Biologie Évolutive,
> Université du Québec à Rimouski,
>
> (418) 723-1986 #1438
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Mon, 13 Sep 2004 14:43:49 -0700
> From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
> Subject: RE: [Histonet] Recover tissue sections on non-plus slides
> To: "Kim Byrnes" <k_byrnes <@t> yahoo.com>, "Histonet (E-mail)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <0BE6ADFAE4E7E04496BF21ABD346628001C5C062 <@t> EXCHANGE1.huntingtonhospital.com
> >
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> We have used the Mount Quick from Newcomer Supply, and it works well.
>
> Laurie Colbert
> Huntington Memorial Hospital
> Pasadena, CA
>
> -----Original Message-----
> From: Kim Byrnes [mailto:k_byrnes <@t> yahoo.com]
> Sent: Monday, September 13, 2004 11:34 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Recover tissue sections on non-plus slides
>
>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
> http://promotions.yahoo.com/new_mail
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 10
> Date: Mon, 13 Sep 2004 20:01:58 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000701c499ee$cb688f70$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Mon, 13 Sep 2004 20:07:19 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000e01c499ee$ee9cdbe0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Mon, 13 Sep 2004 20:07:47 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000f01c499ee$ef064c10$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Mon, 13 Sep 2004 20:10:32 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <001301c499ef$45100dd0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Mon, 13 Sep 2004 20:11:36 -0400
> From: "Robin Turcotte" <robint <@t> mediom.qc.ca>
> Subject: [Histonet] cryomacrocut
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <001c01c499ef$6fce96e0$df8ceccf <@t> client>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Does anyone have an address from a compagny or an hospital who have a used
> cryomacrocut in good condition to be sold.
>
> Thank you
>
> Robin
>
>
>
> ------------------------------
>
> Message: 15
> Date: Mon, 13 Sep 2004 21:15:52 -0400
> From: "Jim Staruk" <jstaruk <@t> masshistology.com>
> Subject: [Histonet] Shandon Hypercenter Xp
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <000201c499f8$87d3cb80$6401a8c0 <@t> yourw04gtxld67>
> Content-Type: text/plain; charset="us-ascii"
>
> I'm looking for someone familiar with the Shandon Hypercenter Xp tissue
> processor. I need some technical advise. Can someone help me? Anyone
> planning to invoice me for their help need not reply.
>
> Thank you
>
> Jim
>
> ________________________
> James E. Staruk, HT(ASCP)
> Mass Histopathology Service
> www.masshistology.com
>
>
>
>
>
> ------------------------------
>
> Message: 16
> Date: Tue, 14 Sep 2004 13:43:17 +1000
> From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
> Subject: [Histonet] Superfrost Slides
> To: "histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000101c49a0c$f9356e50$e1ce080a <@t> wsahs.nsw.gov.au>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Is anyone having troubles with NO staining on some slides on the Ventana
> Benchmark.
> The biggest problem seems to be with the EDTA buffered CC1 retrieval
> solution.
> Not all slides are effected, just an occasional one, but we usually end up
> with little or no staining with anything including the Haematoxylin.
> We use Menzel Superforst Plus and Superfrost Plus Ultra, and both slides
> have shown the effect.
>
> Thanks
> Bill Sinai
> Laboratory Manager
> Tissue Pathology, ICPMR
> Westmead NSW 2145
> Australia
> Ph 02 9845 7774
>
>
> __________________________________________________________________
>
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> ------------------------------
>
> Message: 17
> Date: Tue, 14 Sep 2004 16:31:15 +0530
> From: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>
> Subject: [Histonet] embedding of small artery_paraffin
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <WorldClient-F200409141631.AA31150971 <@t> sctimst.ker.nic.in>
>
> hello galina,
>
> Rat and mouse organs can be cut easily after short processing(using
> acetone and xylene)and then embedding in paraffin wax of low melting point
> (56 C). We have followed the protocols from below given reference
> successfully and same could applied for the small artery also
>
> Theory and practice of Histological techniques (bancroft and steven)
>
> (or)
>
> Manual of histologic staining methods of the armed forces institutes of
> pathology edited by Lee G. Luna, III edition page 16
>
>
> Raghul J
> Histopathology-Implant Biology
> Sree chitra tirunal institute
> Thiruvananthapuram 695 012
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Tue, 14 Sep 2004 16:56:23 +0530
> From: "Dr. Raghul" <raghul <@t> sctimst.ker.nic.in>
> Subject: [Histonet] vascular graft_sectioning difficulty
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <WorldClient-F200409141656.AA56230975 <@t> sctimst.ker.nic.in>
> Content-Type: text/plain; charset="iso-8859-1"
>
> hello members,
>
> We cut 5 µm thin sections of paraffin embedded vascular graft(Dacron) in
> a rotary microtome with disposable blades but the senior tech tells that
> the tissue sections are crumbling and forces us to go for thick sections
> around 10µm but again not with much success.
>
> We can understand that there is not enough tissue adhereing to the
> graft and we are literally cutting the graft. But did anyone had such
> problems sectioning vascular grafts. Any suggestions?
>
>
> regards ,
>
> Raghul J
> scientist
> Histopathology- implant biology division
> Srichitra tirunal institute of medical sciences and technology
> Trivandrum -695 012
> Kerala, India
>
>
>
>
> ------------------------------
>
> Message: 19
> Date: Tue, 14 Sep 2004 07:54:55 -0400
> From: "Dimaano, Nena" <nena.dimaano <@t> stryker.com>
> Subject: [Histonet] Test
> To: <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <20BE9059B6FC2D4B9E8FF2A9E2C4EEBD06210EE4 <@t> HOS2KEXCHCL.howost.strykercorp.c
> om>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Nena Dimaano
> Advanced Technology
> Stryker Orthopaedics
> 325 Corporate Drive
> Mahwah, NJ 07430
> tel: 201-831-5338
> fax: 201-831-6224
> email: Nena.Dimaano <@t> stryker.com
>
>
>
> ------------------------------
>
> Message: 20
> Date: Tue, 14 Sep 2004 07:09:09 -0500
> From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
> Subject: RE: [Histonet] Superfrost Slides
> To: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>, "histonet
> \(E-mail\)"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <JFEMICGBHEGPLAMIJPJPAEHICGAA.JNocito <@t> Pathreflab.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Bill,
> we've had problems also. It could be that the slides might be too changed,
> which is causing the reagents to bead up. Currently, we are using positive
> charged slides from Statlab Medical Products, which I think they get from
> Erie Scientific. Statlab's number is 1-800-442-3573. The may be able to
> direct you to some one closer to you.
>
> Joe Nocito, BS, HT(ASCP) QIHC
> Histology Manager
> Pathology Reference Lab
> San Antonio, TX
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Bill
> Sinai
> Sent: Monday, September 13, 2004 10:43 PM
> To: histonet (E-mail)
> Subject: [Histonet] Superfrost Slides
>
>
>
> Is anyone having troubles with NO staining on some slides on the Ventana
> Benchmark.
> The biggest problem seems to be with the EDTA buffered CC1 retrieval
> solution.
> Not all slides are effected, just an occasional one, but we usually end up
> with little or no staining with anything including the Haematoxylin.
> We use Menzel Superforst Plus and Superfrost Plus Ultra, and both slides
> have shown the effect.
>
> Thanks
> Bill Sinai
> Laboratory Manager
> Tissue Pathology, ICPMR
> Westmead NSW 2145
> Australia
> Ph 02 9845 7774
>
>
> __________________________________________________________________
>
> This electronic message and any attachments may be confidential. If you
> are not the intended recipient of this message would you please delete the
> message and any attachments and advise the sender. Western Sydney
> Area Health Services (WSAHS) uses virus scanning software but excludes
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>
> This email may contain privileged and confidential information intended
> only for the use of the addressees named above. If you are not the
> intended recipient of this email, you are hereby notified that any use,
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> If
> you have received this email in error, please notify WSAHS
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Tue, 14 Sep 2004 14:29:10 +0200
> From: wasielewski.reinhard.von <@t> mh-hannover.de
> Subject: [Histonet] Perferred fixative for animals
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <41470036.30954.8F79EDC3 <@t> localhost>
> Content-Type: text/plain; charset=US-ASCII
>
> Hi Histonetters,
> We are looking for the most convenient and morpholgical satisfying
> fixative for
> mouse and rat tissue beside formaldehyde. We want to stain a wide range of
>
> different immunomarkers but don' t like formalin (therefore) too much.
> Frozen
> sections are too bad in morphology.
> Please send me any suggestions !
>
> Many thanks in advance,
> Reinhard.
>
>
>
> PD Dr. med. Reinhard von Wasielewski
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Tue, 14 Sep 2004 08:56:22 -0400
> From: "Cullen, Kay" <Kay.Cullen <@t> umassmed.edu>
> Subject: [Histonet] RE: Fixative for animals
> To: "Histonet \(E-mail\)" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <B735CCD4E292B440807A7AA8CB61C6CF391DA2 <@t> edunivmail03.ad.umassmed.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Are you busing straight formaldehyde or formaldehyde/glutaraldehyde? I
> get very good results with Bouin's for most tissue.
>
> Kay Cullen
> Research Associate
> Department of Cell Biology
> University of Massachusetts Medical School
> Worcester, MA USA
>
>
>
> ------------------------------
>
> Message: 23
> Date: Tue, 14 Sep 2004 10:05:39 -0400
> From: "Amy Self" <ASelf <@t> gmhsc.com>
> Subject: [Histonet] Histo. Assistant Job Descriptions
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <39836CD6DB61654E8F95A35898C921860A87F3 <@t> exchange.gmhpost.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering if anyone had any job descriptions as well as job requirements
> that they could share with me? We have one but it very minimal as to what
> the histo assistant should and should not do so our lab manager would like
> to see what other hospitals are doing and requiring for this job position.
> Thanks in advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify us immediately by replying to this message and deleting it from
> your computer. Thank you.
>
>
> ------------------------------
>
> Message: 24
> Date: Tue, 14 Sep 2004 09:16:54 -0500
> From: "LaCinda Burchell" <ljb <@t> medicine.wisc.edu>
> Subject: Re: [Histonet] Perferred fixative for animals
> To: <histonet <@t> lists.utsouthwestern.edu>,
> <wasielewski.reinhard.von <@t> mh-hannover.de>
> Message-ID: <s146b728.080 <@t> gwia.medicine.wisc.edu>
> Content-Type: text/plain; charset=US-ASCII
>
> Dear Reinhard,
> We use Prefer fixative from Anatech Ltd, www.anatechltdusa. We have
> beautiful morphology, staining is wonderful (specials and immunos). The
> only down side of Prefer is that it affects the staining of eosinophils.
> We've recently learned how to get around that issue in human tissues,
> but haven't figured it out in rat tissues. Best of luck! Cindy B
>
> LaCinda Burchell, BA, AS, HT(ASCP)
> University of Wisconsin-Madison, Medical School
> Asthma and Allergy Research IHC Lab
> 600 Highland Ave. CSC K4/913
> Madison, Wisconsin 53792
>
> Phone: 608-262-3518
> FAX: 608-263-3746
>
> >>> <wasielewski.reinhard.von <@t> mh-hannover.de> 09/14/04 07:29AM >>>
> Hi Histonetters,
> We are looking for the most convenient and morpholgical satisfying
> fixative for
> mouse and rat tissue beside formaldehyde. We want to stain a wide range
> of
> different immunomarkers but don' t like formalin (therefore) too much.
> Frozen
> sections are too bad in morphology.
> Please send me any suggestions !
>
> Many thanks in advance,
> Reinhard.
>
>
>
> PD Dr. med. Reinhard von Wasielewski
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 25
> Date: Tue, 14 Sep 2004 10:48:20 -0400
> From: "Barbara Lentz" <Barbara_Lentz <@t> dahlchase.com>
> Subject: Re: [Histonet] Recover tissue sections on non-plus slides
> To: <histonet <@t> lists.utsouthwestern.edu>, <k_byrnes <@t> yahoo.com>
> Message-ID: <s146cc7a.059 <@t> dahlchase.com>
> Content-Type: text/plain; charset=US-ASCII
>
> We, too, use the Mount Quick from Newcomer Supply. On a regular basis,
> we remove H&E tissue sections to perform immunos.
> www.newcomersupply.com is their address, Barb
>
> >>> Kim Byrnes <k_byrnes <@t> yahoo.com> 09/13/04 02:34PM >>>
> Does anyone have a method or know of a product that
> can recover or rescue tissue sections that were
> accidentally mounted onto non-plus (non-polarized,
> non-coated) slides? We would like to do immuno on
> these slides, but they consistently fall off of the
> slides.
>
> Any ideas?
>
> Thanks,
>
> Kim
> Georgetown University
>
>
>
> __________________________________
> Do you Yahoo!?
> Yahoo! Mail - 50x more storage than other providers!
> http://promotions.yahoo.com/new_mail
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 26
> Date: Tue, 14 Sep 2004 07:41:17 -0700
> From: "Kari Bradshaw" <kbradshaw <@t> lcpath.com>
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
> To: "Amy Self" <ASelf <@t> gmhsc.com>, <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <JMELLDIIEJFPHMONOAAFIEIACHAA.kbradshaw <@t> lcpath.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Amy Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 27
> Date: Tue, 14 Sep 2004 10:54:03 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: FW: [Histonet] Histo. Assistant Job Descriptions
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000801c49a73$0ee10140$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
> Here is ours.
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kari
> Bradshaw
> Sent: Tuesday, September 14, 2004 9:41 AM
> To: Amy Self; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Amy Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 28
> Date: Tue, 14 Sep 2004 11:33:48 -0500
> From: "Jeffus, Brandon" <JeffusBrandon <@t> uams.edu>
> Subject: [Histonet] rat embryos
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <748D4F173E342D47B69FC23C87E5913C0125E7D4 <@t> EXCHANGE4.ad.uams.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello all... im rather new to immunohistochemistry so bear with me. I'm
> attempting to process some rat embryos for staining with a primary Ab
> and then a Cy3 labeled secondary for visualization of protein
> localization. I'm wondering if the best processing method would be
> frozen sections of the samples or paraffin embedding the samples? Any
> information would be greatly appreciated.
>
>
>
> Brandon C Jeffus
>
> Graduate Student
>
>
>
> ------------------------------
>
> Message: 29
> Date: Tue, 14 Sep 2004 11:40:24 -0500
> From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
> Subject: RE: FW: [Histonet] Histo. Assistant Job Descriptions
> To: "'Nita Searcy'" <NSEARCY <@t> swmail.sw.org>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <000001c49a79$88e006c0$3601a8c0 <@t> brownpathology.net>
> Content-Type: text/plain; charset="us-ascii"
>
>
> OOPS... attachments bad!! Pasting good.
>
>
> Below is our aid job description.
>
> Bonnie Whitaker
>
>
> Job Description for Laboratory/Grossing Aid
>
>
>
>
> Reports To: Laboratory Manager
>
> Pathologists
>
>
>
> Qualifications:
>
>
>
> Education: high school student or have
> diploma
> or GED
>
>
>
> Experience: not required
>
>
>
> Certification: not required
>
>
>
> Personal: The Lab/Grossing Aid must
> demonstrate an ability to listen carefully and follow directions well.
> The
> incumbent must quickly grasp the work-flow of specimen accessioning and
> grossing and become familiar with these procedures. The incumbent must
> demonstrate reasonable intelligence, diligence, accuracy, cooperation,
> neatness, and promptness in keeping with the prestige and image of this
> laboratory. The incumbent takes guidance and instruction from the
> pathologists, with whom they are working, as well as the Laboratory
> Manager
> and Executive Director.
>
> Responsibilities: The Lab/Grossing Aid will be
> responsible for the following duties:
>
> 1. Accessioning specimens and assisting with grossing procedures
> including: cassette labeling, pulling specimens for additional tissue
> submission, and discarding specimens in accordance with policy/procedure
> manual.
>
> 2. Assisting with the performance of routine maintenance and QC
> procedures on equipment to include but not limited to cryostat, grossing
> area, stain setup and filters.
>
> 3. Maintenance of inventory
>
> 4. Routine changing of stains with appropriate records maintenance.
>
> 5. Cleaning of the grossing area and instruments.
>
> 6. Any filing or routine clerical duties necessary.
>
> 7. Other duties as assigned.
>
>
>
>
> General:
>
> 1. The Lab/Grossing Aid shall be responsible for maintaining all
> company
> records of a proprietary or confidential nature secure and confidential
> and
> shall return all lists of clients, potential clients, and all other
> records,
> at Brown & Associates request.
>
> 2. The Lab/Grossing Aid is responsible for representing B&AML in a
> professional and dignified manner befitting the status of the laboratory
> in
> the Houston Medical Community.
>
> 3. The Lab/Grossing Aid will be expected to comply with all policies
> of
> B&AML as stated in the employee handbook and any newly instituted policies
> not specifically stated in the handbook. Will be expected to keep
> assigned
> work area as neat and clean as possible so as to afford the maximum
> functional usage of the assigned area. Will be expected to keep all
> records
> and files available and accessible to authorized personnel at all times.
>
> 4. Lab/Grossing Aid must be in reasonable good health with no record
> of
> chronic illness resulting in undue absence from work.
>
>
>
> I, the undersigned, have carefully read the above job description and
> fully
> understand all that is stated herein. I agree to perform all of the
> duties
> above as well as any other duties deemed necessary by my supervisor within
> the limits described here.
>
>
>
>
>
> _________________________________________
> _____________________
>
>
> EMPLOYEE SIGNATURE
> DATE
>
>
>
>
>
>
>
> _________________________________________
> _____________________
>
> LABORATORY MANAGER DATE
>
>
>
>
>
>
>
>
>
> Bonnie Whitaker
>
> -----Original Message-----
> From: Nita Searcy [mailto:NSEARCY <@t> swmail.sw.org]
> Sent: Tuesday, September 14, 2004 11:30 AM
> To: bwhitaker <@t> brownpathology.com
> Subject: Re: FW: [Histonet] Histo. Assistant Job Descriptions
>
>
>
> Bonnie, I can't view your description- could you fax? 254-724-4391
>
> Thanks!
>
> >>> "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com> 9/14/2004 10:54:03 AM
> >>>
>
> Here is ours.
> Bonnie Whitaker
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]
> <mailto:histonet-bounces <@t> lists.utsouthwestern.edu%5d> On Behalf Of Kari
> Bradshaw
> Sent: Tuesday, September 14, 2004 9:41 AM
> To: Amy Self; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Histo. Assistant Job Descriptions
>
> Here is our job description.
>
> :) Kari Bradshaw, HT(ASCP)
> Laboratory Manager
> Lower Columbia Pathologists
> 1217 14th Ave
> Longview, WA 98632
> (360)425-5620
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On
> <mailto:histonet-bounces <@t> lists.utsouthwestern.edu%5dOn> Behalf Of Amy
> Self
> Sent: Tuesday, September 14, 2004 7:06 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Histo. Assistant Job Descriptions
>
>
>
>
> Good Morning Netters,
>
> We are looking to hire a histology assistant in our lab. I was
> wandering
> if anyone had any job descriptions as well as job requirements that they
> could share with me? We have one but it very minimal as to what the histo
> assistant should and should not do so our lab manager would like to see
> what
> other hospitals are doing and requiring for this job position. Thanks in
> advance, amy
>
>
>
>
>
>
> NOTE:
> The information contained in this message may be privileged, confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering
> this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please
> notify
> us immediately by replying to this message and deleting it from your
> computer. Thank you.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 10, Issue 19
> ****************************************
>
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