[Histonet] synaptophysin
Julie.Sanders <@t> med.va.gov
Julie.Sanders <@t> med.va.gov
Thu Sep 9 11:44:52 CDT 2004
Annette,
When we had our Ventana NexEs we antigen retrieved in a pressure
cooker....put two coplin jars with Cell Marque Trilogy in the cooker, place
your slides in one of them and leave in pressure cooker 10 minutes after it
reaches pressure. Relieve the steam from the cooker, remove the lid and
place the slides in the other coplin jar of Trilogy for 10 minutes then
rinse in APK was and place on the machine, 32 minute antibody time,
with amplification.
Julie Sanders
Supervisor, Anatomic Pathlogy
VAMC, Cincinnati, Ohio
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: Thursday, September 09, 2004 12:37 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 10, Issue 12
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Today's Topics:
1. specificity of Masson's trichrome (Walker/Langdon)
2. needed: used Fisher 166A or 166MP tissue processor (HCS)
3. PCNA - URINARY BLADDER (balajimr <@t> drreddys.com)
4. Re:Splendore-Hoeppli phenomenon (renton louise mrs)
5. Shredded cigarettes OT (renton louise mrs)
6. p75 and Neuro D (Carl Hobbs)
7. How to relax a land snaildefinitively. (Massimo)
8. RE: synaptophysin (Featherstone, Annette)
9. RE: Land snail dissection. (Greg Dobbin)
10. RE: CLIA regs (Michael Fredrickson)
11. RE: Land snail dissection. (Philip Oshel)
12. Re: specificity of Masson's trichrome (Geoff McAuliffe)
13. H pylori IHC controls (Angela Bitting)
14. RE: IHC for Canine Distemper Virus (Connie McManus)
15. Vendors in Toronto (Fran Lemons)
16. RE: synaptophysin (Sebree Linda A.)
17. RE: Land snail dissection. (Margaret Horne)
18. quantitative graded scale for IHC (Carla M Conway)
19. RE: Vendors in Toronto (Bartlett, Jeanine)
20. RE: Land snail dissection. (Philip Oshel)
21. Re: quantitative graded scale for IHC (Greg Dobbin)
22. Re: How to relax a land snail?definitively. (Ford Royer)
23. Histotech position at Buffalo General Hospital
(Featherstone, Annette)
24. Re: Vendors in Toronto (Gayle Callis)
25. RE: Land snail dissection. (Margaret Horne)
----------------------------------------------------------------------
Message: 1
Date: Wed, 8 Sep 2004 23:48:17 -0400
From: "Walker/Langdon" <karrie.langdon <@t> sympatico.ca>
Subject: [Histonet] specificity of Masson's trichrome
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000d01c4961f$db08dc00$8a19fea9 <@t> walkercvzdlx4z>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters,
To clarify my question of the other day: is it true that Masson's trichrome
could potentially stain fibronectin and/or smooth muscle actin, especially
if it is being overexpressed? Is there a histochemical stain for
fibronectin? We have already successfully done IHC,but are trying to
reconcile data obtained with a few different approaches. We are mostly
working in lung.
Thanks to those who have already provided ideas!
Carrie Langdon
Dept. of Pathology
McMaster University
Hamilton, Ontario
Canada
------------------------------
Message: 2
Date: Wed, 8 Sep 2004 21:18:46 -0700
From: "HCS" <int09018 <@t> alphahunt.com>
Subject: [Histonet] needed: used Fisher 166A or 166MP tissue processor
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000701c49624$1a104740$6501a8c0 <@t> hp>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone have an extra Fisher 166MP or 166A. Mine is beyond repair and
would like to find a working unit. Please email directly. Hope the sales
reps do not call, the budget does not allow for new units at this time.
LeRoy Brown HT(ASCP) HTL
HCS
Everson, WA 98247
1-360-966-7300
------------------------------
Message: 3
Date: Thu, 9 Sep 2004 12:04:53 +0530
From: balajimr <@t> drreddys.com
Subject: [Histonet] PCNA - URINARY BLADDER
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFDC5D70C3.D285DDB6-ON65256F0A.00227DAE-65256F0A.0024271C <@t> drreddys.com>
Content-Type: text/plain; charset="US-ASCII"
Dear Histonetters,
It's nice to be back with Histosearch after a short break.
Well, I am facing problem with PCN staining of urinary bladder of rat. We
are using Zymed PCNA staining kit for the same. I am getting non specific
staining where even the nucleus of the muscle layers are also staining. We
are retrieving the antigen by heating the sections in Citrate buffer. But
we are not having this problem in the intestines of same control animals.
Does anybody have experience of PCNA staining with U. bladder so that they
can advise me about this problem. Ofcourse we have increased the blocking
time with serum upto 30 minutes unsuccessfully.
Thanks in advance.
Dr. M.R. Balaji
Dept. Pre clinical safety evaluation,
Discovery research,
Dr. Reddys Laboratories ltd. Bollaram Road,
Miyapur, Hyderabad, 500 049
Andhra Pradseh, INDIA
Email - balajimr <@t> drreddys.com
Tel: 040- 23045439 - Ext.432.
------------------------------
Message: 4
Date: Thu, 09 Sep 2004 09:05:13 +0200
From: "renton louise mrs" <rentonlf <@t> bru.wits.ac.za>
Subject: [Histonet] Re:Splendore-Hoeppli phenomenon
To: Terry.Marshall <@t> rothgen.nhs.uk, histonet <@t> lists.utsouthwestern.edu
Message-ID: <1094713513.8dd9db60rentonlf <@t> bru.wits.ac.za>
Content-Type: text/plain; charset="UTF-8"
Now, if we could only use that in the lyrics of a song along the lines of:
"lung.... has has Hoeppli-splendore thing"
cheers
-----Original Message-----
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
To: "Angela Bitting" <akbitting <@t> geisinger.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Date: Wed, 8 Sep 2004 14:19:44 +0100
Subject: RE: [Histonet] Modified acid fast stain for actinomyces
No modification is needed. Neither does it stain the Actinomyces. Rather,
the club shaped ends of the filaments, being an antigen-antibody complex -
known as Splendore-Hoeppli phenomenon - are stained red. They are not
necessarily present.
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Angela Bitting [mailto:akbitting <@t> geisinger.edu]
Sent: 08 September 2004 13:53
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Modified acid fast stain for actinomyces
Anyone have a recipe for this stain?
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?
------------------------------
Message: 5
Date: Thu, 09 Sep 2004 09:07:28 +0200
From: "renton louise mrs" <rentonlf <@t> bru.wits.ac.za>
Subject: [Histonet] Shredded cigarettes OT
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1094713648.8dd9db60rentonlf <@t> bru.wits.ac.za>
Content-Type: text/plain; charset="UTF-8"
Tobacco powder is still widely used by gardeners as a "natural/organic"
insecticide
-----Original Message-----
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
To: Gayle Callis <gcallis <@t> montana.edu>
Date: Wed, 08 Sep 2004 15:36:28 -0700
Subject: Re: [Histonet] Shredded cigarettes for Land snail dissection.
I think so, I think it is the nicotine that is the narcotic here.
Geoff
Gayle Callis wrote:
> At the risk of too much humor, would chewing tobacco (not used,
> please!) do the job instead of shredding cigarettes. A scientific use
> for SKOL!!
>
> At 10:23 AM 9/8/2004, you wrote:
>
>> Many centuries ago, I forced a snail out of its shell by shredding a
>> pack of
>> cigarettes into a pint of water and dropping the snail into it.
>> Borradaile's THE INVERTEBRATA has instructions for dissecting the
>> European
>> garden snail Helix pomatia (pp. 604-610 in the 4th edition). The
>> book is
>> out of print, but available used.
>>
>> Allen A. Smith, Ph.D.
>> Professor of Anatomy
>> Barry University
>> School of Graduate Medical Sciences
>> Podiatric Medicine and Surgery
>> Miami Shores, Florida
>
>
> Gayle Callis
> MT,HT,HTL(ASCP)
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367 (lab with voice mail)
> 406 994-4303 (FAX)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
_______________________________________________
Histonet mailing list
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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?
------------------------------
Message: 6
Date: Thu, 9 Sep 2004 08:48:06 +0100
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] p75 and Neuro D
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001701c49641$5805c3f0$e8345c9f <@t> Carlos>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Be most grateful if anyone out there can recommend antibodies against those
two proteins, that work in mouse pwx sections. I have got Chemicon's p75 (
MAB 390). The p75 is the low affinity neurotrophin receptor. I have searched
but I obviously am reluctant to buy unless confirmed that they work in pwax.
Thank you
------------------------------
Message: 7
Date: Thu, 9 Sep 2004 12:57:20 +0200
From: "Massimo" <andromeda_tm <@t> libero.it>
Subject: [Histonet] How to relax a land snaildefinitively.
To: "histonet histonet" <histonet <@t> lists.utsouthwestern.edu>
Cc: Dawn Truscott <DayDawning <@t> wideopenwest.com>, Carol McCollough
<CMCCOLLOUGH <@t> dnr.state.md.us>
Message-ID: <I3RT3K$AC3C3A83CD7587A51DD091A4A2707270 <@t> libero.it>
Content-Type: text/plain; charset=iso-8859-1
Torino 09 September 2004
(ITALY)
I would like to thank for your answers at my question about snail.
I also think to do something pleasant and to raise the scientific contents
of someone answers by sending them an Italian recipe on snails with garlic.
Sorry in Italy we use the olive oil. Butter is very dangerous for the heart,
the arteries ( just like the cigarettes smoke) and it also gets fat.
I am afraid but the recipe is in Italian.
But I'm sure if you enter a good Italian restaurant they can cook it.
But...remember... do not forget to bring with you the snails!
With my Best Regard
Massimo Tosi
Graduate Chemical Engineering
PISA University
ITALY
LUMACHE CON L'AGLIO (SNAILS WITH GARLIC)
INGREDIENTI PER 4 PERSONE
1Kg di lumache già spurgate (appena pulite vi consiglio di metterle per più
di 2 ore al sole in un contenitore con abbondante sale sui bordi, le lumache
infatti attirate dal sole e dal sale escono tutte fuori)
4 spicchi di aglio (GARLIC)
1 mazzetto di prezzemolo
olio extravergine di oliva (OLIVE OIL)
sale
pepe
Lessate le lumache per 15 minuti, schiumando con un mestolo forato
("schiumarola" appunto).
A fine cottura, sciacquatele, rimettetele in tegame con acqua pulita
leggermente salata e cuocete per altri 15 minuti.
A questo punto, sgocciolatele (conservate però un po' d'acqua di cottura) e
trasferitele in una casseruola, dove avete fatto soffriggere per poco tempo
l'aglio, il prezzemolo e il pepe, cuocete per pochi minuti e aggiustate di
sale, irrorate con l'acqua di cottura e servite in una zuppiera con del
prezzemolo fresco.
E adesso inizia lo spasso....
buon appetito
------------------------------
Message: 8
Date: Thu, 9 Sep 2004 07:14:17 -0400
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Subject: RE: [Histonet] synaptophysin
To: 'Joe Nocito' <jnocito <@t> satx.rr.com>, JAnorthernexp <@t> cs.com,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<DF6AB9298498D31199CF0008C7E6C1200CF95E8D <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
We antigen retrieve our synaptophysin in Citrate Buffer ph6.0 in a steamer.
We set the steamer with the citrate in it for 70 minutes, at 20 minutes left
we add the slides. After it steams for the final 20 minutes we let it cool
for 20 minutes. We add amplifier and blocker too. Is your primary dilution
correct?
Annette Featherstone HT/MLT Kaleida Health.
-----Original Message-----
From: Joe Nocito [mailto:jnocito <@t> satx.rr.com]
Sent: Wednesday, September 08, 2004 22:02
To: JAnorthernexp <@t> cs.com; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] synaptophysin
Annette,
did you try increasing the time in cell conditioner? Have you tried to
amplify it?
Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: <JAnorthernexp <@t> cs.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, September 08, 2004 7:06 PM
Subject: [Histonet] synaptophysin
> anyone using a ventana stainer and antibodies have a retrieval method
using a
> decloker? Ours stains very light. Antibody is on for 32 mins we did have
it
> set at 16mins. But increasing the time did not prove to make much
> difference. Any other ideas?? The tissue is formalin fixed and paraffin
embedded.
> Thanks
>
> Annette
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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------------------------------
Message: 9
Date: Thu, 9 Sep 2004 09:22:07 ADT
From: "Greg Dobbin" <dobbin <@t> upei.ca>
Subject: RE: [Histonet] Land snail dissection.
To: Histonet <@t> Pathology.swmed.edu
Message-ID: <414020BF.22473.1CC595 <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
Dear Phil (and others),
All this talk of ripping open snail shells, injecting with MgCl2 and
heaven forbid, fixing alive! Does anyone else out there have to work
within the constraints of an Animal Care Committee?
Some of you might be interested to hear that in a an academic
setting, all procedures carried out on research animals (be it
crustacean, fish, avian or mammalian) have to be approved under
guidelines for ethical and humane use and care of animals for
research. For instance, while I have no objection to boiling my
lobsters alive at home, at work, lobsters must me properly
anesthetized prior to humane euthanasia! It's a strange world we
work in by times!
Cheers!
Greg
Date sent: Wed, 08 Sep 2004 15:38:30 -0500
From: Philip Oshel <peoshel <@t> wisc.edu>
To: Histonet <@t> Pathology.swmed.edu
Copies to: Subject: RE: [Histonet] Land snail
dissection.
> Yep, CaCO3.
> Also yes.
> But, why bother with decalification? Just play crab and crack the
> shell open. The snail can be removed alive, if unhappy. I'd put it in
> MgCl2 first, then open the shell and remove the snail, cool it to
> further relax and anesthetize it, inject fixative into the mantle
> cavity (and possibly the hemocoel), then immersion-fix it. For
> sectioning, I'd dissect the snail into smaller pieces to insure
> proper fixation and infiltration paraffin -- they have a very tough,
> muscular foot, and the mantle can be as well.
> The radula that Gayle referred to earlier is mostly keratin, but many
> snails deposit CaCO3 or other minerals (including iron, if I remember
> right) in the tips of the radular teeth -- either way, it will cause
> grief when paraffin sectioning. It'd be better to carefully dissect
> away the radula and mount it whole -- whole mounts of radulae are
> used in molluc taxonomy anyway. If you do want to section the radula,
> you will need to plastic embed it.
>
> Phil
>
> >Out of curiosity - is the shell made of calcium? I'm asking because I
> >really don't know - not a trick question? Isn't a snail out of it's
shell
> >just a slug?
> >(Now THAT is a joke.)
> >
> >
> >Jackie O'
> >
> >
> >Jacqueline M. O'Connor HT(ASCP)
> >Abbott Laboratories
> >Global Pharmaceutical Research and Development
> >Discovery Chemotherapeutics
> >847.938.4919
> >Fax 847.938.3266
> >
> >
> >
> >
> >Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
> >Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> >09/08/2004 11:56 AM
> >
> >
> > To: histonet <@t> pathology.swmed.edu
> > cc:
> > Subject: Re: RE: [Histonet] Land snail dissection.
> >
> >
> >If the snail is small I recomend you to fix the whole snail and after
> >fixation, decalcify it with 10 % EDTA. then process and include the whole
> >snail. Hope this help
> >
> >
> >
> >
> >
> >El dia 08/09/2004 18:23 usted envio el siguiente mensaje:
> >
> >>Date: 8 de Septiembre de 2004 18:23:01
> >
> >>From: "Smith, Allen" <asmith <@t> mail.barry.edu>
> >
> >>Subject: RE: [Histonet] Land snail dissection.
> >
> >>To: gcallis <@t> montana.edu, histonet <@t> pathology.swmed.edu
> >
> >>
> >
> >> Many centuries ago, I forced a snail out of its shell by shredding a
> >pack of
> >
> >> cigarettes into a pint of water and dropping the snail into it.
> >
> >> Borradaile's THE INVERTEBRATA has instructions for dissecting the
> >European
> >
> >> garden snail Helix pomatia (pp. 604-610 in the 4th edition). The book
> >is
> >
> >> out of print, but available used.
> >
> >>
> >
> >> Allen A. Smith, Ph.D.
> >
> >> Professor of Anatomy
> >
> >> Barry University
> >
> >> School of Graduate Medical Sciences
> >
> >> Podiatric Medicine and Surgery
> >
> >> Miami Shores, Florida
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada, C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.
------------------------------
Message: 10
Date: Thu, 9 Sep 2004 08:44:36 -0400
From: "Michael Fredrickson" <mfredrickson <@t> cohenderm.com>
Subject: RE: [Histonet] CLIA regs
To: "Dawn Cowie" <dlcowie <@t> prodigy.net>, "histonet"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <3D55809F621E7C438DA80098CDE3744D10C5E8 <@t> hub.cohenderm.com>
Content-Type: text/plain; charset="iso-8859-1"
For what it is worth, we were once sited by CLIA for lack of adequate
tracking documentation for sending blocks and slides outside of our
facility. We sent them via regular first class US mail and the block was
never received at the other facility.
It is our understanding that there should be a written policy regarding how
these will be send, i.e. FedEx with tracking numbers etc., and how to follow
up to ensure that materials were received at the other facility and a
mechanism to ensure materials are returned.
Michael Fredrickson, Technical Director
Cohen Dermatopathology
Newton, MA
-----Original Message-----
From: DDittus787 <@t> aol.com [mailto:DDittus787 <@t> aol.com]
Sent: Wednesday, September 08, 2004 4:31 PM
To: Dawn Cowie; histonet
Subject: Re: [Histonet] CLIA regs
Clia bows to the most stringent rule committee , so if the cap rules are
more stringent than state or other , clia will go with cap.
Dana
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------------------------------
Message: 11
Date: Thu, 09 Sep 2004 08:22:37 -0500
From: Philip Oshel <peoshel <@t> wisc.edu>
Subject: RE: [Histonet] Land snail dissection.
To: Histonet <@t> Pathology.swmed.edu
Message-ID: <p05210601bd660814fed0@[10.25.102.178]>
Content-Type: text/plain; format=flowed; charset=us-ascii
Greg,
Yep, we've got an Animal Care Committee here at Univ. Wisconsin, and
all the rest. They -- and most such entities in the US -- just don't
care about anything that doesn't have a backbone.
Besides, MgCl2 and cooling *are* anesthesia for snails, as well as
some crustiaceans at least (my own critters, if a I had a job doing
critters, instead of running microscopes for biomedical types).
MS-222 might work on aquatic snails, but not on land ones.
CO2/CO might work on Pulmonates, come to think of it (land snails are
Pulmonates).
I have to agree with anesthetizing lobsters before dropping them in
the boiling water, though -- I've never believed that they don't feel
the boiling.
Phil
>Dear Phil (and others),
>
>All this talk of ripping open snail shells, injecting with MgCl2 and
>heaven forbid, fixing alive! Does anyone else out there have to work
>within the constraints of an Animal Care Committee?
>
>Some of you might be interested to hear that in a an academic
>setting, all procedures carried out on research animals (be it
>crustacean, fish, avian or mammalian) have to be approved under
>guidelines for ethical and humane use and care of animals for
>research. For instance, while I have no objection to boiling my
>lobsters alive at home, at work, lobsters must me properly
>anesthetized prior to humane euthanasia! It's a strange world we
>work in by times!
>Cheers!
>Greg
>
>Date sent: Wed, 08 Sep 2004 15:38:30 -0500
>From: Philip Oshel <peoshel <@t> wisc.edu>
>To: Histonet <@t> Pathology.swmed.edu
>Copies to: Subject: RE: [Histonet] Land
>snail dissection.
>
>> Yep, CaCO3.
>> Also yes.
>> But, why bother with decalification? Just play crab and crack the
>> shell open. The snail can be removed alive, if unhappy. I'd put it in
>> MgCl2 first, then open the shell and remove the snail, cool it to
>> further relax and anesthetize it, inject fixative into the mantle
>> cavity (and possibly the hemocoel), then immersion-fix it. For
>> sectioning, I'd dissect the snail into smaller pieces to insure
>> proper fixation and infiltration paraffin -- they have a very tough,
>> muscular foot, and the mantle can be as well.
>> The radula that Gayle referred to earlier is mostly keratin, but many
>> snails deposit CaCO3 or other minerals (including iron, if I remember
>> right) in the tips of the radular teeth -- either way, it will cause
>> grief when paraffin sectioning. It'd be better to carefully dissect
>> away the radula and mount it whole -- whole mounts of radulae are
>> used in molluc taxonomy anyway. If you do want to section the radula,
>> you will need to plastic embed it.
>>
>> Phil
>>
>> >Out of curiosity - is the shell made of calcium? I'm asking because I
>> >really don't know - not a trick question? Isn't a snail out of it's
shell
>> >just a slug?
>> >(Now THAT is a joke.)
>> >
>> >
>> >Jackie O'
>> >
>> >
>> >Jacqueline M. O'Connor HT(ASCP)
>> >Abbott Laboratories
>> >Global Pharmaceutical Research and Development
>> >Discovery Chemotherapeutics
>> >847.938.4919
>> >Fax 847.938.3266
>> >
>> >
>> >
>> >
>> >Jose Luis Palazon Fernandez <jluis.palazon <@t> icman.csic.es>
>> >Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
>> >09/08/2004 11:56 AM
>> >
>> >
>> > To: histonet <@t> pathology.swmed.edu
>> > cc:
>> > Subject: Re: RE: [Histonet] Land snail dissection.
>> >
>> >
>> >If the snail is small I recomend you to fix the whole snail and after
>> >fixation, decalcify it with 10 % EDTA. then process and include the
whole
>> >snail. Hope this help
>> >
>> >
>> >
>> >
>> >
>> >El dia 08/09/2004 18:23 usted envio el siguiente mensaje:
>> >
>> >>Date: 8 de Septiembre de 2004 18:23:01
>> >
>> >>From: "Smith, Allen" <asmith <@t> mail.barry.edu>
>> >
>> >>Subject: RE: [Histonet] Land snail dissection.
>> >
>> >>To: gcallis <@t> montana.edu, histonet <@t> pathology.swmed.edu
>> >
>> >>
>> >
>> >> Many centuries ago, I forced a snail out of its shell by shredding a
>> >pack of
>> >
>> >> cigarettes into a pint of water and dropping the snail into it.
>> >
>> >> Borradaile's THE INVERTEBRATA has instructions for dissecting the
>> >European
>> >
>> >> garden snail Helix pomatia (pp. 604-610 in the 4th edition). The
book
>> >is
>> >
>> >> out of print, but available used.
>> >
>> >>
>> >
>> >> Allen A. Smith, Ph.D.
>> >
>> >> Professor of Anatomy
>> >
>> >> Barry University
>> >
>> >> School of Graduate Medical Sciences
>> >
>> >> Podiatric Medicine and Surgery
>> >
>> >> Miami Shores, Florida
>
>
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Greg Dobbin
>Pathology Lab
>Atlantic Veterinary College, U.P.E.I.
>550 University Ave.
>Charlottetown, P.E.I.
>Canada, C1A 4P3
>Phone: (902)566-0744
>Fax: (902)566-0851
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Happiness is a journey, not a destination.
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
------------------------------
Message: 12
Date: Thu, 09 Sep 2004 09:35:09 -0700
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] specificity of Masson's trichrome
To: Walker/Langdon <karrie.langdon <@t> sympatico.ca>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4140863D.1020301 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1
Hi Carrie:
While Masson's might (or might not) stain fibronectin and actin, no
one (ie. reviewers of your work) will believe that it is specific
without rigorous testing. If the results with Masson's do not agree with
IHC, I cannot believe that anyone would throw out the IHC result in
favor of the Masson's result.
Geoff
Walker/Langdon wrote:
>Hi Histonetters,
>
>To clarify my question of the other day: is it true that Masson's
trichrome could potentially stain fibronectin and/or smooth muscle actin,
especially if it is being overexpressed? Is there a histochemical stain for
fibronectin? We have already successfully done IHC,but are trying to
reconcile data obtained with a few different approaches. We are mostly
working in lung.
>
>Thanks to those who have already provided ideas!
>
>Carrie Langdon
>Dept. of Pathology
>McMaster University
>Hamilton, Ontario
>Canada
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 13
Date: Thu, 09 Sep 2004 09:42:30 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] H pylori IHC controls
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s14025a5.002 <@t> GHSGWIANW2.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII
Im still looking for a good H pylori control supplier for IHC. Any help?
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave.
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged. It is
intended solely for the addressee. Access to this message by anyone else is
unauthorized. If you are not the intended recipient, any disclosure,
copying, distribution or any action taken, or omitted to be taken, in
reliance on it is prohibited and may be unlawful. If you have received this
message in error, please delete all electronic copies of this message (and
the documents attached to it, if any), destroy any hard copies you may have
created and notify me immediately by replying to this email. Thank you.
------------------------------
Message: 14
Date: Thu, 9 Sep 2004 07:48:14 -0600
From: "Connie McManus" <convmcm <@t> cc.usu.edu>
Subject: RE: [Histonet] IHC for Canine Distemper Virus
To: "'Jan Shivers'" <shive003 <@t> umn.edu>, "'histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c49673$a77a44b0$4a737b81 <@t> Cygnus>
Content-Type: text/plain; charset="us-ascii"
I get mine from VMRD in Pullman WA. Phone: 509/334-5815
website: www.vmrd.com They have a comprehensive online catalogue and
have pretty good customer service. I also noticed USBiological has
CDV also. Phone: 800/520-3011 website: www.usbio.net
Also, CDV cross reacts with human measles virus.
Have fun. (If it isn't fun, it isn't worth it)
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805
email: convmcm <@t> cc.usu.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan
Shivers
Sent: Thursday, September 02, 2004 9:44 AM
To: histonet
Subject: [Histonet] IHC for Canine Distemper Virus
I would greatly appreciate any vendor source suggestions for Canine
Distemper Virus antibody (for use in IHC on FFPE tissue).
Thank you very much in advance.
Jan Shivers
IHC Supervisor
Univ. of Minnesota Veterinary Diagnostic Lab
1333 Gortner Ave.
St. Paul, MN 55108
shive003 <@t> umn.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 09 Sep 2004 10:11:22 -0400
From: "Fran Lemons" <flemons <@t> bhset.org>
Subject: [Histonet] Vendors in Toronto
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s1402c62.036 <@t> bhsmail.baptistoneword.org>
Content-Type: text/plain; charset=US-ASCII
Can someone out there provide me with a list of vendors for the S/C?? I
know I saw one somewhere, but can't locate it.
Thanks in advance.....
Fran Walker
------------------------------
Message: 16
Date: Thu, 9 Sep 2004 09:14:19 -0500
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
Subject: RE: [Histonet] synaptophysin
To: <JAnorthernexp <@t> cs.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D6B654003615874B873E15BA680E2D22F80C75 <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Annette,
We retrieve our Synaptophysin in a decloaker for 2" in either our home made
citrate buffer or Biocare's Bull's Eye and then stain on the NexES for 32".
Ours isn't real intense either. Try decloaking for 3-4". This should help.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: JAnorthernexp <@t> cs.com [mailto:JAnorthernexp <@t> cs.com]
Sent: Wednesday, September 08, 2004 7:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] synaptophysin
anyone using a ventana stainer and antibodies have a retrieval method using
a
decloker? Ours stains very light. Antibody is on for 32 mins we did have
it
set at 16mins. But increasing the time did not prove to make much
difference. Any other ideas?? The tissue is formalin fixed and paraffin
embedded.
Thanks
Annette
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Thu, 9 Sep 2004 11:18:34 ADT
From: "Margaret Horne" <mhorne <@t> upei.ca>
Subject: RE: [Histonet] Land snail dissection.
To: Histonet <@t> Pathology.swmed.edu, Philip Oshel <peoshel <@t> wisc.edu>
Message-ID: <41403C09.25744.8BB06C <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
I used to use soda water to temporarily put Gammarus,a small
crustacean, to sleep; I immersed them in it til they stopped
twitching. I was told it was the CO2 that did it. Had to be careful
not to overdo it and kill them, as I needed to recover them.
As for lobster , I put them in the freezer for about 20 min or
so til they hardly move , and then plunge them in boiling water
head first. They supposedly die before waking up , so the theory
goes. Makes me feel a little better and I don't lift the lid :-).
Lobster goes well with garlic and butter too,
Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
Canada
------------------------------
Message: 18
Date: Thu, 9 Sep 2004 07:22:02 -0700
From: "Carla M Conway" <cmconway <@t> usgs.gov>
Subject: [Histonet] quantitative graded scale for IHC
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF4AFBF1F1.51F4064B-ON88256F0A.004DB875-88256F0A.004ED13D <@t> wr.usgs.gov>
Content-Type: text/plain; charset=us-ascii
Hello,
I would like to devise a quantitative graded scale for IHC results which
would provide more info than just +/- staining.
I have a reference where IHC staining intensity was rated (from pale pink
to dark red) using alkaline phosphatase/Vector-Red, however we are using
Envision+/AEC which seems to be an all or nothing (red or no red) chromogen
with no gradation. We just received ImagePro image analysis software, so
this may be another route to try. Thanks in advance for any comments or
suggestions you may have.
Sincerely,
Carla Conway
Western Fisheries Research Center
Seattle, WA
------------------------------
Message: 19
Date: Thu, 9 Sep 2004 10:42:00 -0400
From: "Bartlett, Jeanine" <JQB7 <@t> CDC.GOV>
Subject: RE: [Histonet] Vendors in Toronto
To: "Fran Lemons" <flemons <@t> bhset.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CB857F6460D42E4AAEA195054A25406C02F5EDCB <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain; charset="US-ASCII"
Go to www.nsh.org and click on NSH Convention. Then choose 2004
Exhibitors.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fran
Lemons
Sent: Thursday, September 09, 2004 10:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Vendors in Toronto
Can someone out there provide me with a list of vendors for the S/C?? I
know I saw one somewhere, but can't locate it. Thanks in advance.....
Fran Walker
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 20
Date: Thu, 09 Sep 2004 10:49:32 -0500
From: Philip Oshel <peoshel <@t> wisc.edu>
Subject: RE: [Histonet] Land snail dissection.
To: Margaret Horne <mhorne <@t> upei.ca>
Cc: Histonet <@t> Pathology.swmed.edu
Message-ID: <p05210603bd662a971ad3@[10.25.102.178]>
Content-Type: text/plain; format=flowed; charset=us-ascii
Gammarus! One of my favorite critters -- did a lot of work on them at
Memorial in St. John's NF. I didn't try soda water, but, yes it
should work fine, and I agree, the CO2 should be what does the job.
Lobster with garlic and butter ... mmm ... amazing how much lobster
is left over to be "disposed of" when doing EM on the sinus gland.
Phil
>I used to use soda water to temporarily put Gammarus,a small
>crustacean, to sleep; I immersed them in it til they stopped
>twitching. I was told it was the CO2 that did it. Had to be careful
>not to overdo it and kill them, as I needed to recover them.
>
> As for lobster , I put them in the freezer for about 20 min or
>so til they hardly move , and then plunge them in boiling water
>head first. They supposedly die before waking up , so the theory
>goes. Makes me feel a little better and I don't lift the lid :-).
>
> Lobster goes well with garlic and butter too,
>
>
> Margaret
>Margaret Horne ,
>Histology Teaching Assistant,
>Dept. of B.SC.,
>Atlantic Veterinary College, U.P.E.I.,
>550 University Ave., Charlottetown,
>P.E.I., C1A 4P3
>Canada
--
Philip Oshel
Supervisor, BBPIC microscopy facility
Department of Animal Sciences
University of Wisconsin
1675 Observatory Drive
Madison, WI 53706 - 1284
voice: (608) 263-4162
fax: (608) 262-5157 (dept. fax)
------------------------------
Message: 21
Date: Thu, 9 Sep 2004 12:54:09 ADT
From: "Greg Dobbin" <dobbin <@t> upei.ca>
Subject: Re: [Histonet] quantitative graded scale for IHC
To: "Carla M Conway" <cmconway <@t> usgs.gov>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <41405270.25853.DEE86B <@t> localhost>
Content-Type: text/plain; charset=US-ASCII
Hi Carla,
I certainly may be corrected by others on this but I personally don't
think you will obtain statistically valid data by grading intensity of the
reaction between cells or fields or sections. There is just so much
room for subjectivity and for that matter, variability between runs or
perhaps even between slides within runs!
What you may want to try is calculating a ratio of positive to
negative (perhaps pos. cells vs. neg. cells in a given area). For
instance: place a grid with 50 or 100 intersects on the computer
monitor, choose a microscopic field (in a random or systematic-
random manner), and then at each intersect decide whether the
reaction at that point is positive or negative. If at any particular
intersect you have trouble to decide whether it is pos or neg, have
a system that does not permit subjectivity, such as: for such points
where the call could go either way, look at the space imediately to
the left of the y-axis and above the x-axis and then make your
decision. Determination of the number of fields you need to sample
in order to obtain statistically valid data is very important and a
matter for a statistician to explain and/or determine for you (which I
most definately am not) unless you happen to have that training
yourself.
Another idea would be to have the image analysis software either
measure the area of or count the number of pixels in a given area
(field) that are "this red or redder". This involves choosing a
relatively arbitrational threshold for the degree of redness that is to
be measured or counted and applying the exact same setting to all
other fields and sections in the study. My personal experience
(using Bioquant NOVA) has been this sounds great in theory but
was very difficult to pull off! The good old-fashioned counting was
much more reliable and for that matter, easier to defend in
presentation or publication.
I am eager to hear other ideas or methods that might also work!
Good luck.
Greg
To: Histonet <@t> lists.utsouthwestern.edu
From: "Carla M Conway" <cmconway <@t> usgs.gov>
Date sent: Thu, 9 Sep 2004 07:22:02 -0700
Copies to: Subject: [Histonet] quantitative
graded scale for IHC
> Hello,
>
> I would like to devise a quantitative graded scale for IHC results which
> would provide more info than just +/- staining.
> I have a reference where IHC staining intensity was rated (from pale pink
> to dark red) using alkaline phosphatase/Vector-Red, however we are using
> Envision+/AEC which seems to be an all or nothing (red or no red)
chromogen
> with no gradation. We just received ImagePro image analysis software, so
> this may be another route to try. Thanks in advance for any comments or
> suggestions you may have.
>
> Sincerely,
>
> Carla Conway
> Western Fisheries Research Center
> Seattle, WA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada, C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.
------------------------------
Message: 22
Date: Thu, 09 Sep 2004 11:05:12 -0500
From: Ford Royer <froyer <@t> bitstream.net>
Subject: Re: [Histonet] How to relax a land snail?definitively.
To: histonet histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <41407F38.3000502 <@t> bitstream.net>
Content-Type: text/plain; charset=windows-1252; format=flowed
Subject: How to relax a land snail...definitively.
Have you tried placing them in a warm bubble bath with subdued lighting,
soft music playing, a little candle light, and a nice Merlot or Chablis
to sip on?
That always relaxes me...
~ Ford ;-)
Ford M. Royer, MT(ASCP)
Midwest Science and Biocenter (MSB)
6551 Jansen Ave. NE, Ste. 102
Albertville, MN 55301
(800) 745-4869 phone
763-497-6728 fax
<froyer <@t> msb-inc.com> email
<http://www.msb-inc.com> Web URL
Massimo wrote:
>Torino 09 September 2004
>(ITALY)
>
>I would like to thank for your answers at my question about snail.
>I also think to do something pleasant and to raise the scientific contents
of someone answers by sending them an Italian recipe on snails with garlic.
>Sorry in Italy we use the olive oil. Butter is very dangerous for the
heart, the arteries ( just like the cigarettes smoke) and it also gets fat.
>I am afraid but the recipe is in Italian.
>But I'm sure if you enter a good Italian restaurant they can cook it.
>But...remember... do not forget to bring with you the snails!
>
>
>With my Best Regard
>
>Massimo Tosi
>
>Graduate Chemical Engineering
>
>PISA University
>ITALY
>
>
>LUMACHE CON L'AGLIO (SNAILS WITH GARLIC)
>
>
>INGREDIENTI PER 4 PERSONE
>
>1Kg di lumache già spurgate (appena pulite vi consiglio di metterle per più
di 2 ore al sole in un contenitore con abbondante sale sui bordi, le lumache
infatti attirate dal sole e dal sale escono tutte fuori)
>4 spicchi di aglio (GARLIC)
>1 mazzetto di prezzemolo
>olio extravergine di oliva (OLIVE OIL)
>sale
>pepe
>
>Lessate le lumache per 15 minuti, schiumando con un mestolo forato
("schiumarola" appunto).
>A fine cottura, sciacquatele, rimettetele in tegame con acqua pulita
leggermente salata e cuocete per altri 15 minuti.
>A questo punto, sgocciolatele (conservate però un po' d'acqua di cottura) e
trasferitele in una casseruola, dove avete fatto soffriggere per poco tempo
l'aglio, il prezzemolo e il pepe, cuocete per pochi minuti e aggiustate di
sale, irrorate con l'acqua di cottura e servite in una zuppiera con del
prezzemolo fresco.
>
>E adesso inizia lo spasso....
>buon appetito
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
------------------------------
Message: 23
Date: Thu, 9 Sep 2004 12:04:22 -0400
From: "Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org>
Subject: [Histonet] Histotech position at Buffalo General Hospital
To: 'Greg Dobbin' <dobbin <@t> upei.ca>, Carla M Conway <cmconway <@t> usgs.gov>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<DF6AB9298498D31199CF0008C7E6C1200CF95E92 <@t> kalmb02.kaleidahealth.org>
Content-Type: text/plain; charset="iso-8859-1"
We currently have 2 histotech positions open at Kaleida Health, Buffalo
General Hospital in sunny Buffalo NY. Two shifts are available 4-12midnight
and 8-4pm. These are full time, with full benefits and a great working
environment. Please contact me if you are interested.
Annette Featherstone HT/MLT
Kaleida Health, Buffalo General Hospital
100 High St.
Buffalo, NY 12403
716-859-2625
FAX: 716-859-1853
-----Original Message-----
From: Greg Dobbin [mailto:dobbin <@t> upei.ca]
Sent: Thursday, September 09, 2004 08:54
To: Carla M Conway
Cc: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] quantitative graded scale for IHC
Hi Carla,
I certainly may be corrected by others on this but I personally don't
think you will obtain statistically valid data by grading intensity of the
reaction between cells or fields or sections. There is just so much
room for subjectivity and for that matter, variability between runs or
perhaps even between slides within runs!
What you may want to try is calculating a ratio of positive to
negative (perhaps pos. cells vs. neg. cells in a given area). For
instance: place a grid with 50 or 100 intersects on the computer
monitor, choose a microscopic field (in a random or systematic-
random manner), and then at each intersect decide whether the
reaction at that point is positive or negative. If at any particular
intersect you have trouble to decide whether it is pos or neg, have
a system that does not permit subjectivity, such as: for such points
where the call could go either way, look at the space imediately to
the left of the y-axis and above the x-axis and then make your
decision. Determination of the number of fields you need to sample
in order to obtain statistically valid data is very important and a
matter for a statistician to explain and/or determine for you (which I
most definately am not) unless you happen to have that training
yourself.
Another idea would be to have the image analysis software either
measure the area of or count the number of pixels in a given area
(field) that are "this red or redder". This involves choosing a
relatively arbitrational threshold for the degree of redness that is to
be measured or counted and applying the exact same setting to all
other fields and sections in the study. My personal experience
(using Bioquant NOVA) has been this sounds great in theory but
was very difficult to pull off! The good old-fashioned counting was
much more reliable and for that matter, easier to defend in
presentation or publication.
I am eager to hear other ideas or methods that might also work!
Good luck.
Greg
To: Histonet <@t> lists.utsouthwestern.edu
From: "Carla M Conway" <cmconway <@t> usgs.gov>
Date sent: Thu, 9 Sep 2004 07:22:02 -0700
Copies to: Subject: [Histonet] quantitative
graded scale for IHC
> Hello,
>
> I would like to devise a quantitative graded scale for IHC results which
> would provide more info than just +/- staining.
> I have a reference where IHC staining intensity was rated (from pale pink
> to dark red) using alkaline phosphatase/Vector-Red, however we are using
> Envision+/AEC which seems to be an all or nothing (red or no red)
chromogen
> with no gradation. We just received ImagePro image analysis software, so
> this may be another route to try. Thanks in advance for any comments or
> suggestions you may have.
>
> Sincerely,
>
> Carla Conway
> Western Fisheries Research Center
> Seattle, WA
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Greg Dobbin
Pathology Lab
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Charlottetown, P.E.I.
Canada, C1A 4P3
Phone: (902)566-0744
Fax: (902)566-0851
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Happiness is a journey, not a destination.
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Message: 24
Date: Thu, 09 Sep 2004 10:23:32 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] Vendors in Toronto
To: "Fran Lemons" <flemons <@t> bhset.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20040909102245.01b541f0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Try NSH website, meeting information should have all this www.NSH.org,
click on convention.
At 08:11 AM 9/9/2004, you wrote:
>Can someone out there provide me with a list of vendors for the S/C?? I
>know I saw one somewhere, but can't locate it.
>Thanks in advance.....
>Fran Walker
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 25
Date: Thu, 9 Sep 2004 13:24:02 ADT
From: "Margaret Horne" <mhorne <@t> upei.ca>
Subject: RE: [Histonet] Land snail dissection.
To: "Massimo" <andromeda_tm <@t> libero.it>
Cc: Histonet <@t> Pathology.swmed.edu
Message-ID: <41405971.8016.FE9066 <@t> localhost>
Content-Type: text/plain; charset="us-ascii"
Massimo,
I am sorry that you do not often have the pleasure of eating
lobster. If you are ever in Prince Edward Island , Canada , then I
will feed you lobster. You might find it an interesting comparison
with the Mediterranean lobster which has a different flavour and
texture.
Thank you for the recipe for escargot; I love them and always
wanted to know how to cook them. I sent the recipe to my
daughter who plans on going to Italy next summer on an
archaeological dig and she sent me back a rough translation,
which you can find in the attachment.
Bon Appetit,
Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3
Canada
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