[Histonet] synaptophysin

Sebree Linda A. la.sebree <@t> hosp.wisc.edu
Thu Sep 9 09:14:19 CDT 2004


We retrieve our Synaptophysin in a decloaker for 2" in either our home made citrate buffer or Biocare's Bull's Eye and then stain on the NexES for 32".  Ours isn't real intense either.  Try decloaking for 3-4".  This should help.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
FAX:  (608)262-7174

-----Original Message-----
From: JAnorthernexp <@t> cs.com [mailto:JAnorthernexp <@t> cs.com]
Sent: Wednesday, September 08, 2004 7:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] synaptophysin

anyone using a ventana stainer and antibodies have a retrieval method using a 
decloker?  Ours stains very light.  Antibody is on for 32 mins we did have it 
set at 16mins.  But increasing the time did not prove to make much 
difference.  Any other ideas??  The tissue is formalin fixed and paraffin embedded.  

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