[Histonet] synaptophysin

[Joe Nocito]

Annette,
did you try increasing the time in cell conditioner? Have you tried to
amplify it?

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: <JAnorthernexp <@t> cs.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, September 08, 2004 7:06 PM
Subject: [Histonet] synaptophysin


> anyone using a ventana stainer and antibodies have a retrieval method
using a
> decloker?  Ours stains very light.  Antibody is on for 32 mins we did have
it
> set at 16mins.  But increasing the time did not prove to make much
> difference.  Any other ideas??  The tissue is formalin fixed and paraffin
embedded.
> Thanks
>
> Annette
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