[Histonet] Resin embedding-bone matrix
Caroline Stott
caroline.stott <@t> anatomy.otago.ac.nz
Wed Sep 8 17:00:26 CDT 2004
Hi all,
I don't think this will work but.... I have been asked to resin embed some
bone that was exposed to treatments of high temps and pressures
etc. Consequently I have been left only with the in-organic matrix. I
cant decalcify it, or embed in paraffin because there will be nothing
left, and I don't think it will cut even if I manage to get it into
resin. One of the samples is just powder!!! (Apparently they are looking
at a particular protein?)
However the question is, should I make my resin mixture harder than normal
to accommodate the hard nature of the tissue? Has anyone tried anything
similar??? Or do you have another medium I can embed into?
Help and many thanks
Caroline
Caroline Stott
Histology Service Unit
University of Otago
PO Box 913
Dunedin, New Zealand
Ph (03) 479 7152
Fax (03) 479 7136
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