[Histonet] Double/Tripple Labelling - Fluorescent

Melanie Black ctsblack <@t> capeheart.uct.ac.za
Wed Sep 8 04:14:35 CDT 2004

Hi There

I know this topic has been around before, so forgive me!!

I am attempting doulble and then tripple fluorescent labelling. Do I 
need to add a fixation step between Primary antibodies, to secure the 
first Ag/Ab complex???

Any help with this proceedure will be greatly appreciated.

Many Thanks
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935

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