[Histonet] Double/Tripple Labelling - Fluorescent
ctsblack <@t> capeheart.uct.ac.za
Wed Sep 8 04:14:35 CDT 2004
I know this topic has been around before, so forgive me!!
I am attempting doulble and then tripple fluorescent labelling. Do I
need to add a fixation step between Primary antibodies, to secure the
first Ag/Ab complex???
Any help with this proceedure will be greatly appreciated.
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
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University of Cape Town
Republic of South Africa
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