[Histonet] plastic in paraffin block

[renton louise mrs]

Hi Wen,

You'll probably find that the wound dressing (sounds like an OpSite dressing) will peel away when exposed to solvent in processing. Fixation alone might be enough to harden the tissue and allow the dressing to be peeled away more easily without damaging the underlying skin.
You could also try to get a sample from the surgeon/hospital/doctor where the tissue originated and do a little test in the lab first. After having tested numerous tape & dressing samples for totally another reason, I have found that most are more or less removed in either alcohol or xylene.

Good luck
-----Original Message-----
From: wen eng <weneng2004 <@t> yahoo.com>
To: histonet <@t> lists.utsouthwestern.edu
Date: Tue, 7 Sep 2004 11:54:39 -0700 (PDT)
Subject: [Histonet] plastic in paraffin block

Hi,
 
I got some skin tissue in formalin that covered with plastic film. If I peel off plastic it will damaged the wound. Looks like I have to process them with the plastic on. But I worried that the plastic will give me big headache when I section them.
 
Anybody has good idea?
 
Thanks in advance!
 
Wen


		
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Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
.......so what IS the speed of dark?