[Histonet] re muscle actin

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Mon Sep 6 03:22:01 CDT 2004

You shouldn't have any problems.....provided that they are not inflammed. 
I've used that AB successfully on mouse etc ( pwax HEIR with citric pH6 or 
TRIS pH10).  Dilution factor around 1/5000, when I use Goat anti mouse, 
biotinylated and StreptABC- Px , Dako. Using their recommended dilutions . 
Std immuno procedure using BSA solution to preblock and dilute the primary 
and secondary Abs. It also works on the pwax sections without HEIR, but not 
as strong, IMHO.
I'll dig out a pic, if you wish

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