[Histonet] re muscle actin
carl.hobbs <@t> kcl.ac.uk
Mon Sep 6 03:22:01 CDT 2004
You shouldn't have any problems.....provided that they are not inflammed.
I've used that AB successfully on mouse etc ( pwax HEIR with citric pH6 or
TRIS pH10). Dilution factor around 1/5000, when I use Goat anti mouse,
biotinylated and StreptABC- Px , Dako. Using their recommended dilutions .
Std immuno procedure using BSA solution to preblock and dilute the primary
and secondary Abs. It also works on the pwax sections without HEIR, but not
as strong, IMHO.
I'll dig out a pic, if you wish
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