[Histonet] re freezing mouse embryos

Carl Hobbs carl.hobbs <@t> kcl.ac.uk
Mon Sep 6 03:00:26 CDT 2004

IMHO, the morphology/histology of sections of unfixed, frozen  embryos ( 
even after fixing the sections after cutting)is inferior to that of fixed, 
then frozen,  tisssues, but should still be easily recognisable.  Either the 
tissue is necrotic or that the freezing process is inadequate, leading to 
ice-crystal artefact. I do fixed and unfixed embryos by the processes you 
describe. What age are your embryos? Do you post-fix the sections? What in?
Best wishes 

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