[Histonet] RE: detection of HRP in FFPE sections- ?antibody

[Patsy Ruegg]

I have done DAB on ffpe tissue's to detect endogenous peroxidase
(myleoperoxidase) with success to identify neutrophils, just don't use
h202 to block for endogenous peroxidase.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of C.M. van
der Loos
Sent: Thursday, October 21, 2004 12:18 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: detection of HRP in FFPE sections- ?antibody


Dear Shaumik,
I guess that HRP was used as tracer in some kind of labeling experiment
in an whole organ, animal or something, followed by fixation and
embedding. 
As you know endogenous peroxidase activity in erythrocytes and
neutrophils perfectly survives the whole embedding circus quite well
(unfortunately to many of us). So would try this with your labeling HRP
as well. There was a Dako antibody (rabbit) available long time ago.
Also at www.biodesign.com. Hope this helps.

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk>
Date  Thu, 21 Oct 2004 16:42:57 +0100 
To  Histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] detection of HRP in FFPE sections- ?antibody 
Hi histonetters,

I was wondering if any of you out there have experience of detection of
HRP in formalin fixed, paraffin embedded tissue.  I assume that the
process of fixation and tissue processing will destroy the enzymatic
activity of HRP, rendering the usual histochemical reagents useless.
Are there antibodies out there that anyone's used?  Thanks a lot for
your help,

Shaumik Adhya



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