[Histonet] RE: detection of HRP in FFPE sections- ?antibody

[C.M. van der Loos]

Dear Shaumik,
I guess that HRP was used as tracer in some kind of labeling experiment in an whole organ, animal or something, followed by fixation and embedding. 
As you know endogenous peroxidase activity in erythrocytes and neutrophils perfectly survives the whole embedding circus quite well (unfortunately to many of us). So would try this with your labeling HRP as well. There was a Dako antibody (rabbit) available long time ago. Also at www.biodesign.com.
Hope this helps.

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands

----- Original Message ----- 
>From  Shaumik Adhya <shaumik.adhya <@t> ucl.ac.uk> 
Date  Thu, 21 Oct 2004 16:42:57 +0100 
To  Histonet <@t> lists.utsouthwestern.edu 
Subject  [Histonet] detection of HRP in FFPE sections- ?antibody 
Hi histonetters,

I was wondering if any of you out there have experience of detection of HRP in
formalin fixed, paraffin embedded tissue.  I assume that the process of
fixation and tissue processing will destroy the enzymatic activity of HRP,
rendering the usual histochemical reagents useless.  Are there antibodies out
there that anyone's used?  Thanks a lot for your help,

Shaumik Adhya