[Histonet] Problem with tissue processing in my VIP

Marion Hiles Marion.Hiles <@t> north-bristol.swest.nhs.uk
Fri Oct 22 06:16:26 CDT 2004

Ditch the zinc formalin and use 10% neutral buffered formalin and it seems
the processing times are a bit short. Try extending the clearing and wax

Bob Quilty
Frenchay Hospital
Bristol UK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Barbara
Sent: 21 October 2004 16:50
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Problem with tissue processing in my VIP

Hi everyone:
We are having problems when we process our tissue specimens in the VIP.
Whenever we process 80+ blocks (our VIP can hold 150 blocks), anything that
is not a small biopsy does not process well (such as uteri, breast tissue,
etc) even with specimens being held overnight or longer in formalin for
We use zinc formalin, ethyl alcohol, Histo-Solv (xylene substitute - which
we have been using for a long time, so I don't think that is the problem)
and ParaPlast Xtra.
Our times once machine starts processing:
Zinc formalin, total of 2 hours 
70% alcohol, 45 minutes
95% alcohol, total of 90 minutes 
100% alcohol, total of 2-1/2 hours
Histo-Solv, total of 2 hours
Paraplast, total of 2 hours, 15 minutes
Agitation is on and so is vacuum.
Any ideas or help will be appreciated. Thanks in advance.
kjnpeppa <@t> yahoo.com

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