[Histonet] Microwave retrieval

[Weems, Joyce]

We were fortunate enough to have some left over capital dollars and have purchased the new Biogenex Retreival Microwave. It is wonderful - saves lots of time and gives consistency in staining! The only thing we would change is to be have smaller containers for instances when we have just a few slides to retreive. But we think its a winner for those of you are looking to purchase it. 

Cheers, 
Joyce


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of marjorie
lehman
Sent: Thursday, October 21, 2004 7:19 AM
To: Amos Brooks; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave retrieval


Here is a Second Opinion!
I tried using a (Household) microwave for a long time and was not happy. After
I got my Decloaking Chamber from Biocare I never looked back! 
Marge

-----Original Message-----
From:	Amos Brooks [SMTP:amosbrooks <@t> earthlink.net]
Sent:	Wednesday, October 20, 2004 8:00 PM
To:	histonet <@t> lists.utsouthwestern.edu
Subject:	[Histonet] Microwave retrieval

Ed,
         I strongly recommend that you don't try to put a coplin jar of 
retrieval solution with slides in it in the microwave and expect any 
consistant results. What happens with this method is you get a spike of 
heat then a temperature drop. Very often there is boil over and your slides 
dry out. Ideally you should be looking for an even temperature for a 
defined length of time that can be well monitored.
         For this reason if you *must* use a microwave it should be a lab 
grade oven that has consistant wattage and temperature control. It would 
also be good to use one of the microwave pressure cookers to improve the 
consistency. (I think Biocare Medical has them ... the guys that sell Borg 
Decloaker).
         My ultimate recommendation is to stick to the tried and true 
waterbath or steamer method. It may take a bit longer but the consistancy 
is better and there is far less variables in the procedure.
Just my opinion,
You know what they say 'bout opinions,
Amos Brooks

At 01:00 PM 10/20/2004, you wrote:
>Message: 10
>Date: Wed, 20 Oct 2004 11:30:26 -0400
>From: "Harris, Ed" <Hared <@t> FGHI.COM>
>Subject: [Histonet] Microwave retrieval
>To: "'histonet <@t> lists.utsouthwestern.edu'"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <BCBDA208D7498449A95432B874A9629752AAE1 <@t> fghmail.fghi.com>
>Content-Type: text/plain;       charset="iso-8859-1"
>
>Can anyone give me the pro's and con's of steamer/pressure (Decloaker) vs.
>microwave retrieval ?
>Our Doc wants us to change from the Decloaker to microwave. Is a household
>microwave adequate or do we need a lab grade unit?
>Does the microwave save time and improve turn around time?
>
>Thanks in advance
>Ed Harris  HT ASCP MT ABB



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.
Thank you. Saint Josephs Health System, Inc.