[Histonet] IHC withquantum dots

[Morken, Tim - Labvision]

<<But the drawback is that you can not use two or more QDs in order to see
colocalization such as nucleus and membrane molecules of the same cell.>>

Actually, you can see several colors at once, depending on if their
emissions fit into the same bandpass filter range. I've seen three color
preparations that are spectacular.

Tim Morken
Lab Vision - Neomarkers
www.labvision.com

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-----Original Message-----
From: Baowei Peng [mailto:pengbw <@t> sjtu.edu.cn] 
Sent: Thursday, October 14, 2004 12:21 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC withquantum dots



We have a fellow in our lab who worked on QDs (quantum dots). So I have
learned that most QDs can be excitated under wave length about between
400-600nm. It means that you can use one certain wave length to excitate
several different QDs which are distinctive in emission wave length (thus
different in color). But the drawback is that you can not use two or more
QDs in order to see colocalization such as nucleus and membrane molecules of
the same cell. 

 

Baowei Peng
1954 Huashan Road
Pharmacy School
Shanghai Jiaotong University
Shanghai, 200030
China