[Histonet] bone decalcification

[Patsy Ruegg]

Jose,
If you are thinking of doing IHC Formic Acid is a good decal to use
(5%).  Decal Chemicals sells a formic acid product called Immunocal.
There are other quicker products that I believe use Nitric acids but IHC
is better with the Formic in my hands.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jose
Luis Palazon Fernandez
Sent: Tuesday, October 12, 2004 3:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bone decalcification


Dear List-members



I have to dacalcify some fish vertebrae in order to see the vertebral
articulation and intervertebral discs, and have to use a quieck method.
I usually decalcify with EDTA but this method is very slow. I would
appreciate your advise. 



Thanks in advance!



José Luis

Universidad de Oriente-Isla Margarita-Venezuela

actualmente en: Instituto de Ciencias Marinas de Andalucia

Puerto Real, Cádiz, España.

email: jluis.palazon <@t> icman.csic.es

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