[Histonet] H. pylori

[Angela Bitting]

We're perplexed. We've been using Dako's H. pylori AB with Proteinase K
antigen retreival for years now and have never had this problem. Our
control is teeming with organisms but some are staining brown and others
are more of a bluish color. I'm sure they are all H. Pyl organisms, but
I can't figure out why they aren't all staining the same.
Dako says they haven't had any complaints about their Lot number. We've
tried different vials from that same Lot # and both act the same.
Trying different dilutions didn't help. Now, we're extending the
Proteinase K time. After this, we're out of ideas.
Help!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 23-00
100 N Academy Ave.
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916


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