[Histonet] Polyester membrane

[Patsy Ruegg]

Has anyone ever used HISTOGEL as a freezing embedding media?  Also, I
was wondering what experiences if any you all have had with processing
for sectioning polyester membranes with cells grown on them.  Frozen,
Paraffin, GMA?????  I am securing the cut out membrane in histogel on
edge and thinking of trying to gently paraffin process and/or freeze
section???
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James
Watson
Sent: Tuesday, October 12, 2004 9:50 AM
To: Kim Merriam; Histonet
Subject: RE: [Histonet] alternates to OCT embedding media


Kim,

Thermo electron sells a m-1 embedding matrix, which is for -12 to -17
degrees.  This works well on mouse brains because of the warmer
temperature needed to section the tissue.  OCT used to be sold for
different temperatures and the optimum temperature for OCT now is below
-17 degree.

James Watson HT, ASCP
Facilities Manager of Histology
Genomics Institute of the Novartis Research Foundation 858-332-4647
jwatson <@t> gnf.org 

-----Original Message-----
From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com] 
Sent: Tuesday, October 12, 2004 7:44 AM
To: Histonet
Subject: [Histonet] alternates to OCT embedding media


Hello all,
 
I was wondering if anyone knew of alternates to OCT-brand cryo-embedding
media.  I know that there are other brands of the stuff, but it is my
understanding that they are all basically made out of the same thing.
Does anyone know of any other media that is used to prepare frozen
blocks?



Kim Merriam
Novartis
Cambridge, MA
		
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