[Histonet] alternates to OCT embedding media

James Watson jwatson <@t> gnf.org
Tue Oct 12 11:49:53 CDT 2004


Thermo electron sells a m-1 embedding matrix, which is for -12 to -17
degrees.  This works well on mouse brains because of the warmer
temperature needed to section the tissue.  OCT used to be sold for
different temperatures and the optimum temperature for OCT now is below
-17 degree.

James Watson HT, ASCP
Facilities Manager of Histology
Genomics Institute of the Novartis Research Foundation
jwatson <@t> gnf.org 

-----Original Message-----
From: Kim Merriam [mailto:kmerriam2003 <@t> yahoo.com] 
Sent: Tuesday, October 12, 2004 7:44 AM
To: Histonet
Subject: [Histonet] alternates to OCT embedding media

Hello all,
I was wondering if anyone knew of alternates to OCT-brand cryo-embedding
media.  I know that there are other brands of the stuff, but it is my
understanding that they are all basically made out of the same thing.
Does anyone know of any other media that is used to prepare frozen

Kim Merriam
Cambridge, MA
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