[Histonet] Re: Ammonia water pH and other bluing solutions

[Gudrun Lang]

Using lithiumcarbonat is the way we do it with staining frozen sections.
In the HE-stainer we have running tapwater. And  the sections are nice.
After haemalaun in special stains we let the slides in tapwater for 3-5 min.

Gudrun Lang


----- Original Message ----- 
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
To: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Cc: "Histonet" <histonet <@t> lists.utsouthwestern.edu>;
<histonet-bounces <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 30, 2004 8:08 PM
Subject: Re: [Histonet] Re: Ammonia water pH and other bluing solutions


> Anyone remember using saturated lithium carbonate?  I could probably use a
> little lithium right now . . . . .
>
> Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step just
> bringing the slides BACK to a neutral pH after treating them with acid
> which makes them purple-ish?  I like the ammonia because it 'seems' to
> make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to
> make them "blah".(Perception is in the eye of the beholder)   Running tap
> water will make the same miracle happen, but it takes longer.
>
>
>
>
>
>
> "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 11/30/2004 12:51 PM
>
>
>         To:     "Histonet" <histonet <@t> lists.utsouthwestern.edu>
>         cc:
>         Subject:        [Histonet] Re: Ammonia water pH and other bluing
solutions
>
>
> Way back in the day, Brigati was using PBS on his immunostainer to blue
> after the hematoxylin counterstain.  I have since used PBS to blue my
> slides instead of ammonium hydroxide/water.  It's cheap, and very easy
> on the sections, and I have plenty of it on hand.
>
>
> Teri Johnson
> Managing Director Histology Facility
> Stowers Institute for Medical Research
> 1000 E. 50th St.
> Kansas City, Missouri  64110
> tjj <@t> stowers-institute.org
>
>
>
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