[Histonet] Re: Ammonia water pH and other bluing solutions

[Robyn Vazquez]

Jackie,
I use a saturated lithium carbonate for my frozen Mohs tissue.  Works
and looks great...
 
Robyn
OHSU

>>> "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com> 11/30/2004
11:08:41 AM >>>

Anyone remember using saturated lithium carbonate?  I could probably
use a 
little lithium right now . . . . . 

Correct me if I'm wrong (Pandora's box) but isn't the "bluing" step
just 
bringing the slides BACK to a neutral pH after treating them with acid

which makes them purple-ish?  I like the ammonia because it 'seems' to

make the nuclei sharper and instantaneously - Li2Co3 and PBS seemed to

make them "blah".(Perception is in the eye of the beholder)   Running
tap 
water will make the same miracle happen, but it takes longer.






"Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
11/30/2004 12:51 PM


        To:     "Histonet" <histonet <@t> lists.utsouthwestern.edu>
        cc: 
        Subject:        [Histonet] Re: Ammonia water pH and other
bluing solutions


Way back in the day, Brigati was using PBS on his immunostainer to
blue
after the hematoxylin counterstain.  I have since used PBS to blue my
slides instead of ammonium hydroxide/water.  It's cheap, and very easy
on the sections, and I have plenty of it on hand.


Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org



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