[Histonet] cell markers for mouse kidney cells?

[Geoff McAuliffe]

Hi Sharon:

    Kidney histology is not that difficult so I don't know why 
identifying cells should be a problem. Mouse kidney does not look all 
that different from human kidney. Are we talking about tubules in the 
cortex or the medulla, blood vessels, glomeruli or interstitial tissue? 
Someone else suggested comparing an adjacent H&E section to the IHC 
section, that seems like the first place to start.
    If you go to your library and get a copy of "The Kidney" by Brenner 
and Rector it has a big chapter on kidney anatomy in vol. 1. Also, the 
"Handbook of Physiology" has a chapter on kidney anatomy. I did my 
dissertation on kidney and I teach histology so if you will send me a 
jpeg image I will see if I can figure it out.

Geoff

Sharon Cooperman wrote:

> Dear Histonetters,
>
> I did immunohistochemistry in mouse kidney and found increased levels 
> of a particular protein in certain cells, but I can't figure out what 
> type of cell the staining is in.  Previously, another person in my lab 
> looking at the same protein even showed the sections to a renal 
> pathologist and was told that the cell type couldn't be determined in 
> the sections they had (which were just IHC for the specific protein 
> and counterstain).  Is there a way to identify specific cell types in 
> kidney using cell type specific markers and does anyone know what they 
> are or have suggestions on what might be useful to try?
>
> Thanks,
> Sharon


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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