[Histonet] cell markers for mouse kidney cells?

Patsy Ruegg pruegg <@t> ihctech.net
Fri Nov 26 13:44:34 CST 2004


Sharon,
An H&E viewed by a good mouse pathologist should be enough to determine what
cell type your protein is expressed in, the other option would be to do
double IHC labeling but you would have to know what possible cell type it
could be to choose the marker.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Sharon
Cooperman
Sent: Friday, November 26, 2004 10:36 AM
To: histonet <@t> Pathology.swmed.edu
Subject: [Histonet] cell markers for mouse kidney cells?


Dear Histonetters,

I did immunohistochemistry in mouse kidney and found increased levels
of a particular protein in certain cells, but I can't figure out what
type of cell the staining is in.  Previously, another person in my
lab looking at the same protein even showed the sections to a renal
pathologist and was told that the cell type couldn't be determined in
the sections they had (which were just IHC for the specific protein
and counterstain).  Is there a way to identify specific cell types in
kidney using cell type specific markers and does anyone know what
they are or have suggestions on what might be useful to try?

Thanks,
Sharon
--
Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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