[Histonet] NALT fixation, decalcification and staining.

[Lindsay Hall]

Hi, 

I want to look for mouse cell antigens in the NALT from decalcified
mouse heads, I know most antibodies to mouse cell surface markers do not
work in FFPE tissues. Any suggestions on how to fix the mouse heads and
decalcify and still be able to stain for CD4, CD8 etc...?

How about Zinc fixation with EDTA decalcification - or will the Zn ions
be chelated?

Cheers

Lindsay